2016, Number 2
Next >>
Rev Cubana Plant Med 2016; 21 (2)
Determination of polyphenols and antioxidant activity of polar extracts of comfrey (Symphytum officinale L)
Nossa GDL, Talero PYV, Rozo NWE
Language: Spanish
References: 23
Page: 125-132
PDF size: 135.66 Kb.
ABSTRACT
Introduction: The Comfrey (
Symphytum officinalis (L.) (fam.
Boraginacea), is a
plant that has been used for therapeutic purposes in popular medicine. It has been
used externally for bruises, joint pains and rheumatic and also its use for treatment of
diseases of the digestive tract. Due to the presence of some hepatotoxic alkaloids, its
use has been restricted only to the external.
Objetive: To determine the content of entire polifenoles and to study the antirust
potential of the polar extracts of the leaves of Comfrey.
Methods: The extracts of the leaves of Comfrey were prepared in different solvents:
methanol - water to 50 %, water and acetate of ethyl. Polyphenol content was then
determined by the Folin-Ciocalteu method, and the antioxidant activity by the
methods of DPPH •+ (1.1-diphenyl-2-picrylhydrazyl) and ABTS•+ (2.2'-azinobis (3-
ethylbenzothiazoline-6-sulfonic acid).
Results: The three studied polar extracts showed antioxidant potential. The methanol
extract showed the highest concentration of polyphenols with a value of 6.93 mg
Gallic acid/g dry extract. This same extract had the highest antioxidant potential by
the two methods, since most power showed radical scavenging.
Conclusions: The antioxidant potential of polar extracts of Comfrey and total
polyphenol content was evident.
REFERENCES
Gomez M, Olivera C, Xavier J, Villano B. Comfrey (Symphytum officinale L.) and experimental hepatic carcinogenesis: A short-term carcinogenesis model study. eCAM. 2010;7(2):197-202.
Adams M, Berset C, Kessler M, Hamburger M. Medicinal herbs for the treatment of rheumatic disorders-A survey of European herbals from the 16th and 17th century. Journal of Ethnopharmacology. 2009;121:343-59.
Foster S, Tyler V. Tyler's Honest herbal: A sensible guide to the use of herbs and related remedies. Routledge. 1999:1-470.
Liu F, Yin Wan S, Jiang Z, Yau Li S, Ong E, Castaño J, et al. Determination of pyrrolizidine alkaloids in comfrey by liquid chromatography-electrospray ionization mass spectrometry. Talanta. 2009;80:916-23.
Steckel F, Seitz H. The efficacy and safety of comfrey. Public Health Nutrition. 2000;3(4A):501-8.
Frost R, MacPherson H, O´Meara S. The external use of comfrey: A practitioner survey. Complementary Therapies in Clinical Practice. 2014:1-9.
Smith D, Jacobson B. Effect of a blend of comfrey root extract (Symphytum officinale L.) and tannic acidcreams in the treatment of osteoarthritis of theknee: randomized, placebo-controlled, double-blind, multiclinical trials. Journal of Chiropractic Medicine. 2011;10:147-56.
Koll M, Dieter R, Pabst H, Predel H, Petrowicz O, Giannetti B, et al. Efficacy and tolerance of a comfrey root extract (Extr. Rad. Symphyti) in the treatment of ankle distorsions: results of a multicenter, randomized, placebo-controlled, double-blind study. Phytomedicine. 2004;11:470-7.
Frost R, MacPherson H, O´Meara S. A critical scoping review of external uses of comfrey (Symphytum spp.). Complementary Therapies in Medicine. 2013;21:724-45.
Kim N, Oberlies N, Brine D, Handy R, Wani M, Wall M, et al. Isolation of Symlandine from the Roots of Common Comfrey (Symphytum officinale) Using Countercurrent Chromatography. J. Nat. Prod. 2001;64:251-3.
Mei N, Guo L, Fu P, Heflich R, Chen T. Mutagenicity of comfrey (Symphytum officinale) in rat liver. British Journal of Cancer. 2005;92:873-5.
Glickman R, Mukherji A. Moxibustion for asthma, acupuncture for epilepsy, psychological therapies for irritable bowel syndrome, exercise training for multiple sclerosis, comfrey root for acute back pain. Explore: The journel of science and healing; 2014. p. 1-5.
Chen T, Fuscoe J, Li Z. MicroRNAs and their predicted target messenger RNAs are deregulated by exposure to a carcinogenic dose of comfrey in rat live. Toxicology Letters. 2011;205:S62.
Bergmana M, Varshavskya L, Gottliebb H, Grossmana S. The antioxidant activity of aqueous spinach extract: chemical identification of active fractions. Phytochemistry. 2001;58:143-52.
Pérez R, Rodríguez C, Lara J, Montes R, Ramírez G. Toxicidad de aceites, esencias y extractos vegetales en larvas de mosquito culex quinquefasciatus say (diptera: culicidae). Acta Zoológica Mexicana. 2004;20(1):141-52.
Dewanto V, Wu X, Adom K, Liu R. Thermal Processing Enhances the Nutritional Value of Tomatoes by Increasing Total Antioxidant Activity. J. Agric. Food Chem. 2002;50:3010-4.
Singh R, Murthy C, Jayaprakasha G. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J. Agric. Food Chem. 2002;50:81−6.
Puertas M, Tobón J, Arango V. Kalanchoe daigremontiana Raym.-Hamet. & H. y su potencial uso como fuente de antioxidantes y colorantes naturales. Revista Cubana de Plantas Medicinales. 2014;19(1):61-8.
Gene E, Kim S, Marjorie L, Medina B, Robert A. Comparative analysis of strawberry total phenolics via Fast Blue BB vs. Folin-Ciocalteu: Assay interference by ascorbic acid. Journal of Food Composition and Analysis. 2012;27:102-7.
Valéria M, Mambro Di, Maria J, Fonseca V. Assays Of Physical Stability And Antioxidant Activity Of A Topical Formulation Added With Different Plant Extracts. Journal of Pharmaceutical and Biomedical Analysis. 2005;37:287-95.
Sanja Ć, Zeljković A, Požgan F, Štefane B, Arkowski P, Maksimović M. Antioxidant activity of natural and modified phenolic extracts from Satureja montana L. Industrial Crops and Products. 2015;76:1094-9.
Babbar N, Oberoi HS, Uppal DS, Patil RT. Total phenolic content and antioxidant capacity of extracts obtained from six important fruit residues. Food Research International. 2011;44:391-6.
Subramanian D. Priyadharshini, Venugopal Sujatha. Antioxidant Profile And Gc-Ms Analysis Of Solanum Erianthum Leaves And Stem- A Comparison. International Journal of Pharmacy and Pharmaceutical Sciences. 2013;5:652-8.