Gutierrez Gutierrez, Reinaldo; Pupo Balboa, Judith; Riverón Forment, Gretel; González Anta, Ana M; Pandolfi Blanco, Anamarys; de Armas Satiesteban, Aimara; Cásido Rodríguez, Mildrey; Rojas Betancourt, Iris

Language: Spanish
References: 15
Page: 36-39
PDF size: 433.81 Kb.

ABSTRACT

Von Hippel-Lindau disease is an autosomal dominant disorder which displays considerable inter- and intra-familial variability in phenotypic expression. VHL is characterized mainly by the development of tumors in various tissues and organs. Comet assay was used in this study to evaluate the basal levels of single stranded breaks in DNA, DNA repair capacity against oxidation induced by H2O2 and endogenous oxidized guanines damage in lymphocyte DNA of VHL patients compared to healthy subjects. No significant differences in DNA damage were observed between controls and patients (p › 0,05), but the DNA repair capacity was less efficient in VHL patients (p ‹ 0.05), and oxidative damage was increased. Using this assay we could identify poor repair capacity and increased oxidized guanine damage in VHL patients.

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