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2016, Number 1

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Rev Cubana Med Trop 2016; 68 (1)

Detection of beta-exotoxins in isolates of Bacillus thuringiensis native to Cuba

González RA, Menéndez DZ, García GI, Anaya MJ, González BR, Calderón CIR, Baró RY, Companioni IA, Gato AR

Language: Spanish
References: 17
Page: 105-110
PDF size: 127.34 Kb.


ABSTRACT

Introduction: Bacillus thuringiensis is a sporulated gram-positive bacterium, well known mainly for its pathogenic action against insects. Some strains synthesize thermostable nucleoside analog secondary metabolites identified as beta-exotoxins. The World Health Organization has recommended that beta-exotoxin-producing Bacillus thuringiensis strains not be used for insect control, due to their unspecific range of action and their toxicity to mammalian cells.
Objective: Detect by means of bioassays the presence of beta-exotoxins in Cuban isolates of Bacillus thuringiensis with entomopathogenic activity against Aedes aegypti (Linneaus, 1762).
Methods: Aaedes aegypti eggs were put in 500 mL water at 37°C until eclosion. Next, 66 mg fish flour were added to 10 mL of the supernatant of the autoclaved culture. Living individuals were counted daily for 7 days. Statistical processing of mortality data was conducted with one-way analysis of variance (ANOVA) and Tukey's multiple comparison. The differences found were considered to be statistically significant for p ‹ 0.05. The presence of beta-exotoxins was directly confirmed by spectrophotometry.
Results: The presence of beta-exotoxins was detected by the two methods applied in only one isolate (A51).
Conclusions: Absence of beta-exotoxins in 11 isolates with marked entomopathogenic activity against Aedes aegypti confirms their potential for the development of new biolarvicides.


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