Bisset LJA, Rodríguez CMM, Hurtado ND, Hernández AH, Valdéz MV, Fuentes LI

Language: Spanish
References: 27
Page: 82-94
PDF size: 232.67 Kb.

ABSTRACT

Introduction: The program for control of Aedes aegypti (L.) (Diptera: Culicidae) in Cuba is mainly based on the use of temephos (Abate) as larvicide and pyrethroids as adulticides. Organophosphate (OP) insecticide Chlorpyrifos has also been used on occasion. Monitoring resistance to these insecticides is essential to achieve effective control of the species.
Objective: Determine temephos resistance in larvae and its mechanisms, and evaluate the level of resistance to the insecticides most commonly used as adulticides in five health areas from the municipality of Boyeros, Havana, surveyed in the years 2010 and 2012.
Methods: An evaluation was conducted of resistance to and effectiveness of temephos in its commercial formulation (Abatex G1) in larvae, using tests recommended by the World Health Organization. Resistance mechanisms were assessed with biochemical assays. The impregnated bottle bioassay was used to determine resistance in the adult stage.
Results: Larval resistance to temephos decreased from 2010 to 2012. The commercial product temephos showed 100% mortality between 5 and 12 days. Esterases and monooxygenases were found to play an important role in larval resistance to temephos. Some strains showed resistance to pyrethroids and Chlorpyrifos in the adult stage.
Conclusions: These results corroborate the need to set up integrated control strategies to preserve the useful life of the insecticides available to control Aedes aegypti in the municipality of Boyeros.

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