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2016, Number 1

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Rev Cubana Plant Med 2016; 21 (1)

Modulatory activity of ethanolic extracts of the leaves of Clusia nemorosa G. Mey (Clusiaceae) on antimicrobial drugs

Honorio OA, Oliveira AA, Cortez SVL, Pessoa SMA, Melo CHD, Figueiredos SMA

Language: Portugués
References: 19
Page: 1-8
PDF size: 126.04 Kb.


ABSTRACT

Introduction: the fast development of drug resistance and the slowdown of the development of new active drugs, drew attention to the treatment with the drug combination.
Objective: to analyze the antimicrobial activity of the ethanol extract of Cluisa nemorosa (Clusiaceae) against multiresistant strains and standards as well as the modulating action with aminoglycoside amikacin, gentamicin and neomycin.
Methods: the plant material (leaves) was collected in Araripe, crushed and put into ethanol 96 % as solvent. Distillation of the solvent was then performed in a rotary evaporator to produce the gross ethanol extrato. A microdilution assay was conducted to verify antibacterial activity and the possible interactions of aminoglycosides associated with the study samples. A sub-inhibitory concentration of 128 µg/mL (Minimum Inhibitory concentration/8) was used.
Results: the activity of the extract against the strains isolated patterns had an Minimum Inhibitory concentration › 1024 mg/mL, the action of antibiotics was modulated synergistically by the extract against multidrug-resistant Gram-positive bacteria Staphylococcus aureus and Gram-negative Escherichia coli and Pseudomonas aeruginosa.
Conclusions: the synergism of the ethanol extract was verified by microdilution method. Therefore, it is suggested that the extract of Clusia nemorosa be used as a source of natural products for antimicrobial therapy and to combat bacterial multidrug resistance.


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