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2016, Number 1

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Rev Cubana Invest Bioméd 2016; 35 (1)

Effect of combining nutrient bases and inhibitor on recovery of Salmonella

López RY, Zhurbenko R, Rodríguez MC

Language: Spanish
References: 41
Page: 24-35
PDF size: 159.95 Kb.


ABSTRACT

Introduction: Salmonella are the most common causes of food-borne disease worldwide. Selenite cystine broth is a selective enrichment medium used for the recovery of Salmonella species in samples of food, water, feces and other materials of clinical importance.
Objective: Evaluate the combination of nutrition bases obtained by original methods and sodium selenite to ensure appropriate recovery of Salmonella species in selenite cystine broth.
Methods: A comparative study was conducted with various nutrition bases to evaluate the fostering of bacterial growth in six strains of Salmonella and Shigella. Two variants were prepared and the microorganisms selected were inoculated at an approximate concentration of 3 × 108 colony-forming units per milliliter. Absorbance was measured with a 640 nanometer spectrophotometer every hour to determine biomass increase. Behavior of the selenite cystine broth medium formulated by ingredients was compared with that of Merck cystine selenite broth in the presence of the microorganisms of interest. Determination was performed of the productivity and selectivity of the culture medium.
Results: The variant containing the mixture of nutrient bases (bacteriological peptone Z, soybean peptone and casein enzymatic hydrolysate) facilitated better recovery of the strains tested, with significant differences (p ‹ 0.05) with respect to the variant containing casein enzymatic hydrolysate alone. The experimental medium containing the mixture of nutrient bases and sodium selenite displayed Salmonella recovery at low concentrations in a manner similar to the reference medium and inhibited Escherichia coli more efficiently at low concentrations. At high concentrations neither medium was able to inhibit the growth of E. coli. Productivity was satisfactory in both media, ranging between 0.1 and 1, and so was selectivity, which reached an approximate value of 3.
Conclusions: The combination of original nutrition bases and sodium selenite allowed appropriate recovery of Salmonella species.


REFERENCES

  1. Uribe C, Suárez MC. Salmonelosis no tifoidea y su transmisión a través de alimentos de origen aviar. Colombia Médica. 2006;37:151-8.

  2. Shahunja KM, Leung DT, Ahmed T, Bardhan PK, Ahmed D, Qadri F, et al. Factors Associated with Non-typhoidal Salmonella Bacteremia versus Typhoidal Salmonella Bacteremia in Patients Presenting for Care in an Urban Diarrheal Disease Hospital in Bangladesh. PLoS Negl Trop Dis [Versión electrónica]. 2015 [citado octubre 2015];9. Disponible en: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004066

  3. Newman KL, Leon JS, Rebolledo PA, Scallan E. The impact of socioeconomic status on foodborne illness in high-income countries: a systematic review. Epidemiology and Infection. 2015;143:2473-85.

  4. WHO Fourth formal meeting of the Foodborne Disease Burden Epidemiology Reference Group (FERG). World Health Organization, Geneva, Switzerland; 2014.

  5. WHO. Consultation to develop a strategy to estimate the global burden of foodborne diseases. World Health Organization, Geneva, Switzerland; 2007.

  6. Kopper G, Calderón G, Schneider Sh, Domínguez W, Gutiérrez G. Enfermedades transmitidas por alimentos y su impacto económico. Estudios de caso en Costa Rica, El Salvador, Guatemala, Honduras y Nicaragua. Informe técnico sobre ingeniería agrícola y alimentaria. Organización de las Naciones Unidas para la Agricultura y la Alimentación (FAO); 2009.

  7. Alerte V, Cortés AS, Díaz TJ, Vollaire ZJ, Espinoza MME, Solari GV, et al. Brotes de enfermedades transmitidas por alimentos y agua en la Región Metropolitana, Chile (2005-2010). Rev Chil Infectol. 2012;29:26-31.

  8. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, et al. Foodborne Illness Acquired in the United States - Major Pathogens. Emerg Infect Dis. 2011;17:7-15.

  9. Whitney BM, Mainero C, Humes E, Hurd Sh, Niccolai L, Hadler JL, et al. Socioeconomic Status and Foodborne Pathogens in Connecticut, USA, 2000-2011. Emerg Infect Dis. 2015;21:1617-24.

  10. De Jong B, Ekdahl K. The comparative burden of salmonellosis in the European Union member states, associated and candidate countries. Bmc Public Health [Versión electrónica]. 2006 [citado en octubre 2015];6. Disponible en: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1352352/#

  11. Sundström K, Wahlström H, Ivarsson S, Lewerin SS. Economic effects of introducing alternative salmonella control strategies in Sweden. Plos One [Versión electrónica]. 2014 [citado octubre 2015];9. Disponible en: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096446

  12. Bernal KHSH, Iglesias EJB. Salmonelosis extraintestinal: Reporte de caso. Investigaciones Medicoquirúrgicas. 2015;6:305-11.

  13. Puig Peña Y, Robert Maceo BA, Leyva Castillo V. Epidemiological factors of interest in outbreaks of food-borne diseases in Havana. Rev Cubana Hig Epidemiol. 2013;51:262-8.

  14. Puig Peña Y, Espino Hernández M, Leyva Castillo V, Aportela López N, Machín Díaz M, Soto Rodríguez P, et al. Serovariedades y patrones de susceptibilidad a los antimicrobianos de cepas de Salmonella aisladas de alimentos en Cuba. Rev Panam Salud Pública. 2011;30:561-5.

  15. Agren ECC, Johansson J, Frössling J, Wahlström H, Emanuelson U, Sternberg- Lewerin S, et al. Factors affecting costs for on-farm control of salmonella in Swedish dairy herds. Acta Veterinaria Scandinavica. 2015;57:28.

  16. Ramírez-Rueda RY, Rincón DP, Vargas J. Salmonella Enteritidis en huevos de gallina comercializados en Tunja (Colombia). Salud Soc Uptc. 2014;12:22-7.

  17. Morales PR, Avalos de la Cruz DA, Leyva-Ruelas G, Ybarra Moncada MaC. Calidad bacteriológica de leche cruda de cabra producida en Miravalles, Puebla. Revista mexicana de ingeniería química. 2012;11:45-54.

  18. Barrera-Escorcia G, Fernández-Rendón CL, Wong-Chang I, Ramírez Romero P. La sensibilidad del grupo coliforme como indicador de la presencia de enterobacterias patógenas en cuatro cuerpos acuáticos de México. Hidrobiológica. 2013;23:87-96.

  19. Sharath K, Archana D, Dhanashree B. Comparison of culture media for the isolation of salmonella spp. from stool samples. Jarbs. 2012;4:30-4.

  20. Cuartas Trujillo MC, Molina Upegui OL, Restrepo Ceballos AC, Maya Carmona CY, Jaramillo Veláquez S, Donado Gómez JH, et al. Sensibilidad y especificidad del recuento de leucocitos en las materias fecales para predecir la presencia de Salmonella o Shigellaen pacientes con enfermedad diarreica aguda. Iatreia. 2008;21:5-12.

  21. Rodríguez Martínez C, Zhurbenko R, Quesada Muñiz VdJ, Lobaina Rodríguez T, Tsoraeva A, Díaz Pérez M, et al. Manual de Medios de Cultivo. 3ra ed. BioCen ISBN 959-7160-25-0. La Habana, Cuba; 2004. p. 265.

  22. SIGMA. Biochemicals and Reagents for Life Science Research; 2000. p. 1534.

  23. ISO 11133-2. Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media - Part 2: Practical guidelines on performance testing of culture media. ISSO. Geneva; 2003.

  24. Arcos-Ávila EC, Mora-Cardona L, Fandiño-de Rubio LC, Rondón-Barragán Iang S. Prevalencia de Salmonella spp. en carne porcina, plantas de beneficio y expendios del Tolima. Orinoquia. 2013;17:59-68.

  25. Miller S, Zieger U, Ganser C, Satterlee SA, Bankovich B, Amadi V, et al. Influence of land use and climate on Salmonella carrier status in the small Indian mongoose (Herpestes auropunctatus) in Grenada, West Indies. Journal of Wildlife Diseases. 2015;51:60-8.

  26. Rodríguez Torrens H, Barreto Argilagos G, Sedrés Cabrera M, Bertot Valdés J, Martinez Sáez S, Guevara Viera G, et al. Los alimentos de origen porcino: vehículos predominantes en las salmonelosis camagüeyanas. Rev Prod Anim. 2011;23:109-12.

  27. Rodríguez R, Fandiño C, Donado P, Guzmán L, Verjan N. Characterization of Salmonella from commercial egg-laying hen farms in a central region of Colombia. Avian Dis. 2015;59:57-63.

  28. Martínez Vasallo A, Villoch Cambas A, Ribot Enriquez A, Montes de Oca N, Riverón Alemán Y, Ponce Ceballo P. Calidad e inocuidad en la leche cruda de una cadena de producción de una provincia occidental de Cuba. Rev Salud Anim. 2015;37:79-85.

  29. Muñoz S, Vilca L, Ramos D, Lucas J. Frecuencia de enterobacterias en verduras frescas de consumo crudo expendidas en cuatro mercados de Lima, Perú. Rev Investig Vet. 2013;24:300-6.

  30. Noor Uddin GM, Larsen MH, Barco L, Minh Phu T, Dalsgaard A. Clonal Occurrence of Salmonella Weltevreden in Cultured Shrimp in the Mekong Delta, Vietnam. Plos one. 2015 [citado octubre 2015];10. Disponible en: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134252

  31. Rahimi E, Shakerian A, Falavarjani AG. Prevalence and antimicrobial resistance of Salmonella isolated from fish, shrimp, lobster, and crab in Iran. Comp Clin Pathol. 2013;22:59-62.

  32. Zhurbenko R. Metodología para el aprovechamiento de los subproductos de la industria alimenticia y otras proteínas en la evaluación de la calidad sanitaria de los alimentos [Tesis doctoral]. Habana: Universidad de La Habana; 2005.

  33. Bridson EY. The OXOID Manual. 8th ed. OXOID Limited. U.K.: Wade Road, Basingstoke; 1998.

  34. Zhurbenko R, Rodríguez Martínez C, Díaz Pérez M, Durán Vila A, López Hernández OD, Viera Oramas DR, et al. Caracterización de la peptona de soya para el cultivo de microorganismos. Rev Cubana Med Trop. 2006 [citado octubre 2015];58. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375- 07602006000200003&lng=es"lng=es

  35. Murray PR, Rosenthal KS, Faüer MA. Microbiología médica. 5ta ed. Madrid: Elsevier; 2009.

  36. Tsoraeva A, Zhurbenko R. Development and characterization of a mixed nutrient base for the culture of a wide range of microorganisms. Rev Latinoam Microbiol. 2000;42:155-61.

  37. Khan H, Flint SH, Yu PL. Development of a chemically defined medium for the production of enterolysin A from Enterococcus faecalis B9510. J Appl Microbiol. 2013;114:1092-102.

  38. Fallah M, Bahram S, Javadian SR. Fish peptone development using enzymatic hydrolysis of silver carp by-products as a nitrogen source in Staphylococcus aureus media. Food Science & Nutrition. 2015;3:153-7.

  39. Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Wenn WC. Color Atlas Textbook of Diagnostic Microbiology. 5th ed. Philadelphia, New York: Lippincott; 1997.

  40. Vasic S, Radojevic I, Pešic N, Comic L. Influence of sodium selenite on the growth of selected bacteria species and their sensitivity to antibiotics. Kragujevac J. Sci. 2011;33:55-61.

  41. Merck. Manual de medios de cultivo. Darmstadt, Alemania: Editorial Merck; 1982.




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