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2016, Number 2

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Rev Med Inst Mex Seguro Soc 2016; 54 (2)

Identification of Mycobacterium aviumintracellulare complex by PCR of AIDS and disseminated mycobacteriosis

García-Elorriaga G, Degollado-Estrada E, Villagómez-Ruiz A, Cortés-Torres N, Arreguín-Reséndiz L, del Rey-Pineda G, González-Bonilla C

Language: Spanish
References: 34
Page: 170-175
PDF size: 324.13 Kb.


ABSTRACT

Objective: The aim of this study is to differentially identify MAC by PCR in patients with AIDS and disseminated mycobacteriosis.
Methods: A cross sectional study was conducted in Mexico to identify MAC by Molecular Biology. Two sets of primers were synthesized: MAV and MIN, for M. avium and M. intracellulare, respectively. Whole-cell DNAs obtained from 29 clinical isolates and clinical serum specimens from other 24 patients with AIDS and disseminated mycobacterial infection were extracted and amplified by PCR with the MAV and MIN primers. The MAV and MIN primers each amplified one highly specific 1.3-kb segment of the homologous DNA, respectively.
Results: Twenty-nine DNAs from MAC clinical isolates identified by Gen-Probe AccuProbes were amplified with the MAV primers. Of the 24 clinical samples, 3 were positive for M. avium and 6 for M. tuberculosis.
Conclusions: Our results demonstrated that PCR technique could be applied for the differentiation of M. avium and M. intracellulare by specific 16S rRNA primers. In patients with advanced stage AIDS and in whom disseminated mycobacteriosis is suspected, the presence of anemia (even with negative cultures), elevated alkaline phosphatase and a median CD4 count of 15.9/mL, the diagnosis of infection by MAC should be strongly considered; we suggest that in accordance with our findings, a more precise stratification of patients in terms of their CD4 T cell counts is warranted.


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