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2016, Number 1

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Gac Med Mex 2016; 152 (1)

Assessment of the concentrations of carbonylated proteins and carbonyl reductase enzyme in mexican women with breast cancer: A pilot study

Gutiérrez-Salinas J, García-Ortiz L, Mondragón-Terán P, Hernández-Rodríguez S, Ramírez-García S, Núñez-Ramos NR

Language: Spanish
References: 20
Page: 13-18
PDF size: 74.20 Kb.


ABSTRACT

Oxidative stress could promote the development of cancer and implicate carbonylated proteins in the carcinogenic process. The goal of this study was to assess the concentrations of carbonylated proteins and carbonyl reductase enzyme in women with breast cancer and determine whether these markers were possible indicators of tissue damage caused by the disease. A total of 120 healthy women and 123 women with a diagnosis of breast cancer were included. The concentration of carbonylated proteins in plasma and the concentration of carbonyl reductase enzyme in leukocytes were determined using the ELISA assay. There was a 3.76-fold increase in the amount of carbonylated proteins in the plasma from the patient group compared with healthy control group (5 ± 3.27 vs. ± 2.31 nmol carbonyls/mg protein; p ‹ 0.05). Additionally, a 60% increase in the carbonyl reductase enzyme was observed in the patient group compared with the healthy control group (3.27 ± 0.124 vs. 2.04 ± 0.11 ng/mg protein; p ‹ 0.05). A positive correlation (r = 0.95; p ‹ 0.001) was found between both measurements. These results suggest the presence of tissue damage produced by cancer; therefore, these parameters could be used to indicate tissue damage in cancer patients.


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