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2015, Number 2

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RCAN 2015; 25 (2)

Antimicrobial resistance of coagulase positive strains of staphylococci isolated from foods and food handlers

Puig PY, Espino HM, Leyva CV, Apórtela LN, Pérez MY, Soto RP

Language: Spanish
References: 31
Page: 245-260
PDF size: 304.23 Kb.


ABSTRACT

Rationale: Intoxication by staphylococci is frequent among food-borne diseases (FBD). It is worrisome the antimicrobial resistance exhibited by coagulase-positive strains of staphylococci involved in FBD outbreaks. Objective: To establish the antimicrobial resistance phenotype of coagulase-positive strains of staphylococci isolated from food or present in food handlers. Methods: Resistance of 317 isolates of coagulase-positive Staphylococcus spp. isolated either from foods (89.0%) or food handlers (11.0%) to several antimicrobials were assayed at the institution´s Department of Microbiology between November 2010 and December 2012. These activities are mandated as part of the sanitary surveillance and control of FBD outbreaks in different regions of Cuba. Susceptibility to drugs of common clinical use was determined by the Kirby-Bauer method. In the case of S. aureus strains, resistance to meticilline was additionally assayed after screening in agar plate with oxacilline. Minimum inhibitory concentrations were determined by broth microdilution in 28 resistant isolates to this drug. Results: S. aureus was the predominant strain (86.4%), followed by S. intermedius (11.0 %), S. schleiferi subsp.coagulans (1.6 %) and S. hycus (0.9 %). Fifty-six point one percent of isolates were resistant to at least one of the antibiotics tested. Penicillin (52.9%), erythromycin (30.3%) and tetracycline (24.4%) showed the higher percentages of antimicrobial resistance. A fifth of the S. aureus strains were resistant to methicillin. Sixty-two point nine percent of isolated strains were multirresistant. Antimicrobial resistance patterns involved from one up until 5 drugs. Conclusions: S. aureus was the main strain isolated. A high proportion of isolates resistant drugs of common use was observed. The wide diversity of resistance patterns found, as well as the high percentage of multidrug resistant strains, calls for the necessity to promote the rational use of antimicrobials, the adoption of programs for hygiene and prevention of FBD, and the conduction of more in-depth epidemiological studies.


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