medigraphic.com

2012, Number 2

<< Back Next >>

Rev Mex Ing Biomed 2012; 33 (2)

Study of the Biological Activity of Melanoma-cells on a Culture Medium by Means of the Biospeckle Technique

Martínez-Celorio RA, González-Peña RJ, Cibrián RM, Salvador R, Gil-Benso R, San Miguel T, Sánchez-Muñoz OL

Language: Spanish
References: 23
Page: 117-126
PDF size: 886.54 Kb.


ABSTRACT

We are reporting a study of the response of a biological tissue with a malignant melanoma induced of the cell line MEL-RC08 by means of Biospeckle's technique. The study was performed on four prepared samples, which were placed in plastic wells prepared for such purposes. The used samples were: RPMI-1640 Gel; RPMI- 1640 plus 105 cells of the cell line MEL-RC08, and the other two plastic wells was added a concentration of 1 mg and 2 mg of Colcemid drug, respectively. The samples were tested at the same time by analyzing the temporal evolution of speckle patterns in each of them. For that, we designed an experiment, which consisted of to illuminate the samples with an He-Ne laser and acquire a set of 96 images for 24 hours by using a high resolution camera. Next, a temporal di erence method for processing dynamic speckle patterns was implemented with the aim of analyzing the biological activity. The obtained results show that exist a change in the behavior of the biological activity of the samples depending on concentration of the applied drug. The advantages and disadvantages of the method are discussed.


REFERENCES

  1. Goodman J.M. Speckle and Metrology"; Chap. 8 in Speckle phenomena in optics: theory and application, Roberts and Company Publishers, Greenwood Village CO (2007).

  2. Sirohi RS. Speckle Metrology". Marcel Dekker, New York (1993).

  3. Martnez-Celorio R.A., Davila A., Kaufmann G. H.and Mendiola G. Extension of the displacement measurement range for the electronic speckle-shearing pattern interferometry using carrier fringes and a temporal phase unwrapping method". Optical Engineering 2000; 39(3): 751-757.

  4. Rabal H.J. y col., Transient phenomena analysis using dynamic speckle patterns". Optical Engineering 1995; 35(1): 57-62.

  5. Xu Z., Joenathan C., Khorana B.M. Temporal and spatial properties of the time-varying speckles of botanical specimens". Optical Engineering 1995; 34: 1487-1502.

  6. Carvalho P.H.A. y col., Motility parameters assessment of bovine frozen semen by biospeckle laser (BSL) system". Biosystems Engineering 2009; 102(1): 31- 35.

  7. Serra J. Aplicacion de la Morfologa Matematica a la telemedicina y la Biotecnologa: Caracterizacion Morfologica de Celulas de sangre y Analisis de cDNA Microarrays" en Reconocimiento de Patrones Avances y Perspectivas, editado por J. L. Daz de Leon y S. C. Yanez Marquez del Centro de Investigacion en Computacion Instituto Politecnico de Computacion Mexico., 1, 39-50.(2002).

  8. Ayala G., Daz M E., Martnez-Costa M. Granulometric moments and corneal endothelium status". Pattern Recognition 2001; 34(6): 1219-1227.

  9. Braga Jr R A, Rabelo GF, Granato LR, Santos EF, Machado JC, Arizaga R, y col. Detection of fungi beans by the laser biospeckle technique". Biosystems Engineering 2005; 91(4):465-9.

  10. Jardim D. F y col., Observing bacterial activity interferometrically". European Biophysics Journal 2003; 32(2): 159-162.

  11. Machado R.R. y col., Metabolic activity interferometer: description and calibration of an interferometric method to measure growth of mycobacteria". European Biophysics Journal 2008; 38(1): 111-119.

  12. Oulamara A., Tribillon G. and Duvernoy J. Biological activity measurements on botanical specimen surfaces using a temporal decorrelation e ect of laser speckle". Journal of Modern Optics 1989; 36: 165-179.

  13. Braga Jr R A, Olivera B, Rabelo G, Marques R, Machado A, Cap N, y col. Reliability of biospeckle image analysis". Optics and Lasers in Engineering 2007; 45: 390-395.

  14. Sanchez-Arevalo F. M., Aldama-Reyna W., Lara-Rodriguez A.G., Garca- Fernandez T., Pulos G., Trivi M., Villagran-Muniz M. Use of time history speckle pattern and pulsed photoacoustic techniques to detectthe selfaccommodating transformation in a Cu-Al-Ni shape memory alloy". Materials Characterization 2010; 61: 518-524.

  15. Haralick R., Shanmugam M.K. and Dinstein L. Textural features for images classi cation". IEEE Transactions on System, Man and Cybernetics. SMC-3 1973.

  16. Unser M. Sum and Di erence Histogram". IEEE Transactions on Pattern Analysis and Machine Intelligence 1986; 8(1): 118-125.

  17. Fernandez M., Mavilio A., Wavelet transform analysis of dynamic speckle patterns texture". Applied Optics 2002; 41(32): 6745-6750.

  18. Mart-Lopez L., Cabrera H., Martnez- Celorio R.A., Gonzalez-Pe~na R. Temporal di erence method for processing dynamic speckle patterns". Optics Communications 2010; 283(24): 4972-4977.

  19. Gil-Benso R, Monteagudo C, Cerda- Nicolas M, Callaghan RC, Pinto S, Martnez-Romero A, Pelln-Carcelen A, San-Miguel T, Cigudosa JC, Lopez- Gines C., Characterization of a new human melanoma cell line with CD133 expression". Human Cell 2012; 25(2): 61- 7.

  20. Gonzalez R. and Woods R., Digital Image Processing", 3rd. Edition. Prentice-Hall, 2008.

  21. Wang Y.J., Liu H.L., Guo H.T., Wen H.W., Liu J. Primary hepatocyte culture in collagen gel mixture and collagen sandwich". World Journal of Gastroenterology 2004; 10(5): 699-702.

  22. Yang H., Ganguly A., Cabral F. Inhibition of Cell Migration and Cell Division Correlates with Distinct E ects of Microtubule Inhibiting Drugs". The Journal of Biological Chemistry 2010; 285(42): 32242-32250.

  23. Berne J.L., Chueca-Pazos M., Herraez J. Teora y errores de instrumentacion", Ed. 1, Editorial: Thomson Paraninfo, S.A., ISBN: 8428323089, 1996.




2020     |     www.medigraphic.com