2002, Number 2
<< Back Next >>
Rev Inst Nal Enf Resp Mex 2002; 15 (2)
Preserved canine arteries as didactic material for vascular anastomosis procedures.
Sotres-Vega A, Olmos-Zúñiga JR, Jasso-Victoria R, Gutiérrez-Marcos LM, Franco-Oropeza A, Santillán-Doherty P
Language: Spanish
References: 14
Page: 78-83
PDF size: 145.29 Kb.
ABSTRACT
Introduction: Students in vascular surgery and microsurgery courses in the departments of experimental surgery of the Instituto Nacional de Enfermedades Respiratorias and the Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán” use plastic tubes with different caliber as didactic material to perform vascular anastomosis. This plastic material has very different texture when is compared to an actual blood vessel.
Objective: To evaluate the usage of preserved arterial segments in the training of surgical and microsurgical procedures of vascular anastomosis.
Material and methods: Canine arterial segments with variable diameter were obtained from thoracoabdominal aorta, pulmonary artery, femoral arteries and carotid arteries. All arteries were preserved. Students performed vascular anastomoses using preserved arteries and they answered some questions about texture, consistency and the force of the suture through the wall of the preserved vessel in comparison with a normal vessel.
Results: Thirty-six arterial segments were obtained; 18 arteries were preserved using dried-freezing and 18 were cryopreserved; then, 1-cm segments were obtained and randomly supplied to the students for the vascular anastomosis procedure. They were asked if these vessels had consistency and texture similar to a normal vessel and which was more resistant.
Conclusion: The preserved arterial segments demonstrated to be a suitable biological material for surgical or microsurgery training that presented both texture and consistency similar to normal vessels.
REFERENCES
Especificaciones técnicas para la producción, cuidado y uso de animales de laboratorio de la Norma Oficial Mexicana NOM-062-ZOO-1999. Diario Oficial de la Federación. Diciembre 6, 1999. Estados Unidos Mexicanos.
Guide for the care and use of laboratory animals. USA. Department of Health and Human Services. Public Health Service. National Institutes of Health Rev 1985.
Goodman A, Goodman L, Rall T, Murad F. Las bases farmacológicas de la terapéutica. Buenos Aires, Argentina: Médica Panamericana, 1987.
Memories of the Course of Animal Anesthesia and Models of the 12th Annual Meeting and 1st International Conference of the Academy of Surgical Research. University of Muenster Department of Surgery. Muenster, Germany, October, 1996.
Rey L. Basic aspects and future trends in the Freeze-Drying of pharmaceuticals. Dev Biol Stand 1992;74:3-8.
Pratt MF, Schneider JG. Microsurgical application of Frezze-Dried arterial allografts. Laryngoscope1986;96:625-629.
Pratt MF, Shneider JG, Galey FR. Experimental Freeze-Dried microarterial allografts in rabbits. Arch Otolaryngol Head Neack Surg 1987; 113:953-958.
Chow SP, So YC, Chan CW. Experimental microarterial grafts: Frezze-Dried versus autografts. Br J Plast Surg 1983;36:345-347.
Pratt MF, Schneider JG, Norris MS. Microsurgical application of Frezze-Dried venous allografts. Microsurgery 1985; 6:211-218.
Schweinitzer M. Cryopreservation: A useful technique for storing tissues for pharmacological investigations. TIPS 1988;9:221-223.
Mestres AC, Mulet J, Pomar JL. Large caliber cryopreserved arterial allografts in vascular reconstructive operations. Ann Thorac Surg 1995;60:105S-107S.
Mitchell NR, Jonas AR, Schoen F. Structure-Function correlations in cryopreserved allograft cardiac valves. Ann Thorac Surg 1995;60:108S-113S.
Arch E, Saltijeral J, Zarco I, Poblano A. El uso y producción de modelos animales en la investigación científica biomédica. Animales de experimentación 1996;1:10-12.
Santillán-Doherty P, Jasso-Victoria R, Sotres-Vega A, Olmos-Zúñiga R, Vanda B. El animal de laboratorio. Rev Inst Nal Enf Resp Mex 1995;8:243-248.