2015, Number 5
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salud publica mex 2015; 57 (5)
Evaluation of a reduced panel of leptospira strains for microagglutination
Jacob P, Schmeling MF, Chiani YT, Landolt NY, Scialfa E, Fusco S, Vanasco B
Language: Spanish
References: 25
Page: 419-425
PDF size: 234.86 Kb.
ABSTRACT
Objective. To evaluate if the use of the 19 Leptospira strains
panel suggested by the International Leptospirosis Society of
World Health Organization for microagglutination allows
confirmation of more cases that the 12 strains panel used
in Argentina.
Materials and methods. Cross-sectional
observational study. We studied 441 serum samples corresponding
to Argentinean patients with suspected leptospirosis
derived during from July to December, 2009 and
from January to October, 2013.
Results. The same number
of positive samples was obtained using the MAT with the 19
or 12 strains. In six cases a serovar of the expanded collection
was presumably infecting, but always coagglutinated with
strains of the reduced panel.
Conclusion. In Argentina, the
diagnosis of leptospirosis by MAT could be made using the
reduced 12 strains panel, obtaining the same result in case
detection as using the 19 strains panel. Additional information
provided by the use of all strains could be the presumably
infecting serogroup.
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