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2015, Number 2

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Rev Cubana Plant Med 2015; 20 (2)

Cytotoxic action of the stem aqueous extract of the stem of Cereus jamacaru DC. (mandacaru)

Moura SD, de Sousa CFR, Moura AG, Martins L, Tinheiro FPM, Peron AP

Language: English
References: 24
Page: 226-234
PDF size: 115.56 Kb.


ABSTRACT

Introduction: Cereus jamacaru DC. (Cactaceae), with popular name mandacaru, is a plant from the Brazilian semiarid region with medicinal properties against various diseases.
Objective: to evaluate the effect of different concentrations of crude aqueous extracts obtained from C. jamacaru on the cell cycle of meristematic root cells of Allium cepa L.
Methods: the C. jamacaru aqueous extracts were prepared by trituration of pieces from inside the cladodes immersed in distilled water. The concentration considered optimal for the treatment of diseases is 500/2 L. From this concentration were more established other two 500/1.5 and 500/1 L. Each concentration was evaluated at times of 24 and 48 hours exposure. A group of five bulbs of A. cepa was used. The root tips were collected and fixed in acetic acid (3:1) for 24 hours. The slides were prepared by crushing and stained with 2% acetic orcein. Data analysis was performed using the Chi-square (p ‹ 0.05).
Results: the results showed that the concentrations of 500/1.5 and 500/1 g/L have an antiproliferative effect and induced a statistically significant number of cellular aberrations in the test system cells in question.
Conclusion: under the conditions studied, these two concentrations present as cytotoxic.


REFERENCES

  1. Frescura VD, Laughinghouse IV, Tedesco SB. Antiproliferative effect of the tree and medicinal species Luehea divaricata on the Allium cepa cell cycle. Caryologia. 2012;65(1):27-33.

  2. Asare GA, Bugyei K, Sittie A, Yahaya ES, Gyan B, Adjei S, et al. Genotoxicity, cytotoxicity and toxicological evaluation of whole plant extracts of the medicinal plant Phyllanthus niruri (Phyllanthaceae). Genet Mol. 2012;11:100-11. doi: 10.4238/2012.January.13.3.

  3. Taylor N, Zappi D. "Cacti of eastern Brazil." New York: Kew: Royal Botanic Gardens; 2004. p. 499.

  4. Meiado MV, Albuquerque LSC, Rocha EA, Rojas Aréchiga M, Leal IR. Seed germination responses of Cereus jamacaru DC. ssp. jamacaru (Cactaceae) to environmental factors. Plant Species Biol. 2010;25(2):120-8.

  5. Davet A, Carvalho JLS, Dadalt RC, Virtuoso S, Dias JFGD, Miguel MD, et al. Cereus jamacaru: a non buffered LC quantification method to nitrogen compounds. Chromatographia. 2009;69(2):245-7.

  6. Resende GM, Cavalcanti NB. Efeito de diferentes substratos no desenvolvimento de mandacaru (Cereus jamacaru DC.), facheiro ( Pilosocereus pachycladus), xiquexique (Pilosocereus gounellei Bly.) e coroa-de-frade (Melocactus bahiensis). Rev Caatinga. 2007;20(1):28-35.

  7. Guedes RS, Alves EU, Gonçalves EP, Bruno RLA, Braga Junior JM, Medeiros MS, et al. Germinação de sementes de Cereus jamacaru DC. em diferentes substratos e temperaturas. Acta Sci Biol Sci. 2009;31(2):159-4. doi: 10.4025/actascibiolsci. v31i2. 635.

  8. Magalhães A. Perfil etnobotânico e conservacionista das comunidades do entorno da reserva natural Serra das almas, Ceará-Piauí, Brasil. Fortaleza; 2006. p. 68.

  9. Aremud AD, Finnie JF, Standen JV. Potential of south african medicinal plants used as anthelmintics. South African J Bot. 2012;82:134-50.

  10. Bruton LL. As bases farmacológicas da terapêutica. Rio de Janeiro: MCCRAWHILL Brasil; 2006. p. 300.

  11. Messias JB, Caraciolo MCM, Oliveira VRM, Bastos IVGA, Guerra MO. Evaluation hematological and biochemical parameters of rats in the second thirds of the gestation submitted to the action of the extract methanol of Cereus jamacaru DC., Cactaceae. Rev Bras Farmacog. 2010;20(4):478-83.

  12. Sabini MC, Cariddi LN, Escobara FM, Bachetti RA, Sutil SB, Contigiani MS, et al. Evaluation of cytogenotoxic effects of cold aqueous extract from Achyrocline satureioides by Allium cepa L. test. Nat Prod Commun. 2011;6(7):995-8.

  13. Meyer L, Quadros KE, Zeni ALLB. Etnobotânica na comunidade de Santa Bárbaro Ascurra, Santa Catarina, Brasil. Rev Bras Bioc. 2011;10(3):258-66.

  14. Herrero O, Perez JM, Fernández PF. Toxicological evaluation of three contaminant of emerging concern by use of Allium cepa test. Mut Res. 2012;743(1):24-34.

  15. Caritá R, Marin Morales MA. Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of seeds to industrial effluents contaminated with azo dyes. Chemosphere. 2008;72(5):722-5.

  16. Bagatini MD, Silva ACF, Tedesco SB. Uso do sistema-teste Allium cepa como bioindicador de genotoxicidade de infusões de plantas medicinais. Rev Bras Farmacogn. 2007;18(3):509-16.

  17. Fachinetto JM, Bagatini MD, Durigon ACFS, Tedesco SB. Efeito anti-proliferativo das infusões de Achyrocline satureioides DC (Asteraceae) sobre o ciclo de celular de Allium cepa L. Rev Bras Farmacogn. 2007;17:49-54.

  18. Portal Educação. Homeopatia e Fitoterapia [Acessada em jan 2014] Disponível em: http://www.portaleducacao.com.br/farmacia/cursos/1836/curso-de-fitoterapiaehomeopatia? gclid=CIrd66rH5bsCFYl9OgodtngAyQ

  19. Guerra M, Souza MJ. Como observar os cromossomos: um guia de técnicas em citogenética vegetal, animal e humana. Ribeirão Preto: FUNPEC; 2002. p. 201.

  20. Fachinetto JM, Tedesco SB. Atividade antiproliferativa e mutagênica dos extratos aquosos de Baccharis trimera(Less.) A. P. de Candolle e Baccharis articulata (Lam.) Pers. (Asteraceae) sobre o sistema teste de Allium cepa. Rev Bras Pl Med. 2007;11(4):360-7.

  21. Ayres M. BioEstat 5.0: Aplicações estatísticas nas áreas das ciências biológicas e médicas. Belém: Sociedade Civil Mamirauá Brasília, CNPq; 2007.

  22. Souza IA, Lima MCA, Melo UBC, Higino JS. Antitumour properties of Cereus jamacaru on a experimental model of cancer in: fundamental & Clinical Pharmacology. 3rd Meeting of the federation of the European Pharmacological Societies, Lyon, France. 2001;15:400.

  23. Schwarz A, Medeiros I, Mourão C, Queiroz F, Pflugmacher S. Phytochemical and toxic analysis of an ethanol extract from Cereus jamacaru. Toxicology Latters. 2010;196:S344.

  24. Carvalho AC, Balbino EE, Maciel A, Perfeito JP. Situação de registro de medicamentos fitoterápicos no Brasil. Rer Bras Farmacogn. 2008;18(2):314-9.




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