2015, Number 2
Elaboration of a method for vegetative propagation of Catharanthus roseus (L.)
Language: Spanish
References: 32
Page: 200-211
PDF size: 142.44 Kb.
ABSTRACT
Introduction: Catharanthus roseus var. roseus (L.), is a prized plant because it has several antitumor alkaloids.Objective: to evaluate the effect of indoleacetic acid, cutting types, plant age and substrates on Catharanthus roseus (L.) rooting.
Methods: the experiment was arranged in a complete randomized design with a 2 x 2 x 3 factorial with three replications, with two ANA concentrations (0 and 1000 ppm), two positions in the stem (terminal and subterminal) and three types of substrate (commercial substrate, local low fertility soil and sand) in plants aged less and more than a year. After 60 days, percentage of rooted cuttings, root number and length, and percentage of sprouting were evaluated. A nutritional analysis of leaf blades was performed.
Results: the best scores in terms of rooting rates were obtained for the sand, the subterminal portion of the branch and without the rooting promoting substance, in younger plants. Older cuttings best development was found in commercial substrate. No statistical differences were found between cutting types or with or without the rooting promoting substance. Root length and number, as well as sprouting were significantly better using younger cuttings. Nitrogen, Zinc and Manganese content were significantly superior in older plants, but Potassium was higher in the younger ones.
Conclusion: the results obtained demonstrate that the potential of C. roseus to be propagated by cuttings is possible, and that is even better if subterminal young cuttings, in sand, without rooting promoters are used.
REFERENCES
Aslam J, Haque Khan S, Hameed Siddiqui Z, Fatima Z, Maqsood M, Ahmad Bhat M, et al. Catharanthus roseus (L.) G. Don. An important drug: it´s applications and production. Pharmacie Globale (IJCP). [Internet]. 2010 [citado 10 Ene 2014];1(4):1-16. Disponible en: http://www.pharmacieglobale. info/index.php?option=com_docman&task=doc_download&gid=71&Itemid=49
Alor Chávez MJ, Gómez Álvarez R, Huerta Lwanga E, Pat Fernández JM, González Cortázar M, De la Cruz González C, et al. Nutrición y crecimiento en fase de vivero de Catharanthus roseus (L.) G. Don, Mimordica charantia L. y Axadirachta indica A. Juss, en el Municipio Centro, Tabasco–México. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. [Internet]. 2012 [citado 12 Ene 2014];11(2):163-71. Disponible en: http://www.rhsm.usach.cl/ojs/index.php/blacpma/article/download/558/526
Gomes de Moraes L, Alonso AM, Oliveira-Filho EC. Plantas medicinais no tratamento do cáncer: uma breve revisão de literatura. Universitas: Ciências da Saúde, Brasília. [Internet]. 2011 [citado 12 Feb 2014];9(1):77-99. Disponible en: http://www.publicacoes.uniceub.br/index.php/cienciasaude/article/viewArticle/1308
Loyola-Vargas VM, Sánchez-Iturbe P, Canto-Canché B, Gutiérrez-Pacheco L, Glaz-Ávalos R, Moreno-Valenzuela O, et al. Biosíntesis de los alcaloides indólicos. Una revisión crítica. Rev de la Sociedad Química de México. [Internet]. 2004 [citado 9 Feb 2014];48(1):67-94. Disponible en: http://www.jmcs.org.mx/PDFS/V48/03%20Jul%202004/48.1-112/M-Alcaloides.pdf
Sottomayor M, Lopes Cardoso I, Pereira LG, Barceló A. Peroxidase and the biosynthesis of terpenoid indole alkaloids in the medicinal plant Catharanthus roseus (L.) G. Don. Phytochemistry Reviews. [Internet]. 2004 [citado 14 Feb 2014];3:159-71. Disponible en: http://link.springer.com/article/10.1023%2FB%3APHYT.0000047807.66887.09#page-1
Aroucha Santos MC, Paiva Freitas S, Aroucha Santos AL, Mendes Aroucha EM, Souza MS, Silva Lemos GC, et al. Efeito do ácido indolbutírico, tipos de estacas e substratos sobre o enraizamento de Catharanthus roseus. Revista Ceres. [Internet]. 2007 [citado 23 Abr 2014];54(314):369-74. Disponible en: http://www.redalyc.org/articulo.oa?id=305226821008
Schroeder MA. Una alternativa a los cultivos tradicionales en el NO de la provincia de Corrientes. Medición de biomasa y análisis químico de tejidos en plantines micorrizados de Aloysia polystachya (Grisebach) Moldenke y Lippia turbinata Grisenbach. [Tesis de Maestría] Universidad Nacional del Nordeste. Corrientes, Argentina; 2009.
Andrade SAL, Malik S, Sawaya ACHF, Bottcher A, Mazzafera P. Association with arbuscular mycorrhizal fungi influences alkaloid synthesis and accumulation in Catharanthus roseus and Nicotiana tabacum plants. Acta Physiologiae Plantarum. [Internet]. 2013 [citado 23 Ene 2014];35(3):867-80. Disponible en: http://www.bv.fapesp.br/en/publicacao/62579/
Gaur S, Kaushik P. Biodiversity of Vesicular Arbuscular Mycorrhiza associated with Catharanthus roseus, Ocimum spp. and Asparagus racemosus in Uttarakhand State of Indian Central Himalaya. International Journal of Botany. [Internet]. 2011 [citado 23 Ene 2014];7(1):31-41. Disponible en: http://scialert.net/abstract/?doi=ijb.2011.31.41
Zeng Y, Guo L, Chen B, Hao Z, Wang Y, Huang L, et al. Arbuscular Mycorrhizal Symbiosis for Sustainable Cultivation of Chinese Medicinal Plants: A Promising Research Direction. The American Journal of Chinese Medicine. [Internet]. 2013 [citado 10 Ene 2014];41(6):1199–221 Disponible en: http://www.ncbi.nlm.nih.gov/pubmed/24228596