2015, Number 2
<< Back Next >>
Rev Cubana Plant Med 2015; 20 (2)
Phytochemical screening of extracts and 20 % tinctures from root and bark of Dichrostachys cinerea L. (Marabú)
Rodés RS, Peña FD, Hermosilla ER
Language: Spanish
References: 28
Page: 156-166
PDF size: 193.63 Kb.
ABSTRACT
Introduction: the plant
Dichrostachys cinerea L. (Marabú) is an important fodder
for sheep and goats mainly during the drought period. It is traditionally used as anti-parasitic, antifungal, anti-inflammatory, antiseptic and hypoglycemic agent.
There are also some reports about the use of the bark for the treatment of elephantiasis and dysentery. On the other hand root infusions are used to treat syphilis and gonorrhea.
Objective: to identify the secondary metabolites of biological interest present in
D. cinerea species trough the phytochemical screening of extracts and tinctures prepared from bark and root.
Methods: representative parts of the plant were collected in the locality from Barrio Nuevo, Reparto Céspedes, Manzanillo municipality, Granma province, Cuba
and this was identified by PhD. Luis Catasús Guerra, specialist from Cupaynicú Botanical Garden, Guisa municipality. Bark of stems and roots were washed,
disinfected, dried and ground. Ethereal, ethanolic and aqueous extracts as well 20% tinctures were prepared later by ultrasound assisted extraction. A small portion of the extracts was used for the phytochemical screening and the results were confirmed by TLC by thin-layer chromatography analysis.
Results: the phytochemical screening of bark and root extracts and tinctures from
D. cinerea reveals the presence of a great variety of secondary metabolites. Saponins, alkaloids, coumarins and tannins stand out among all the identified metabolites as the more important and abundant. They are in all probability
responsible for the biological properties ascribable to
D. cinerea.
Conclusions: saponins, alkaloids, coumarins and tannins present in
D. cinerea
could be the metabolites responsible for the therapeutic effects ascribable to this plant.
REFERENCES
Satyajit DS, Lutfun N. An Introduction to Natural Products Isolation. En: Satyajit DS, Lutfun N. Natural Products Isolation, Methods in Molecular Biology. 3rd ed. UK: Humana Press Inc. 2012;864:2–3.
Jesse WHL, Vederas JC. Drug discovery and natural products: end of an era or an endless frontier? Science. 2009;325:161–65.
Arece J, Roche Y, López Y, Molina M. Efecto in vitro del extracto acuoso de Dichrostachys cinerea (L.) Wight & Arn. en el desarrollo de las fases exógenas de estrongílidos gastrointestinales de ovinos. Pastos y Forrajes. 2012;35(3):301–9.
Sueiro ML, Ribalta V, González Y, Hernández E. Evaluation of diuretic activity from Dichrostachys cinerea (L.) Wight & Arn. leaves and bark aqueous extracts. VacciMonitor. 2010 [citado 8 Abr 2014];19(2). Disponible en: http://www.finlay.sld.cu/publicaciones/vaccimonitor/Vm2010/vm2010.htm#n5
Omukhulu Neondo J, Mweu Mbithe C, Kariuki Njenga P, Wangari Muthuri C. Phytochemical characterization, antibacterial screening and toxicity evaluation of Dichrostachys cinerea. International J. of Med. Plant Res. 2012;1(4):32–7.
Roig Mesa JT. Plantas medicinales, aromáticas y venenosas de Cuba. La Habana: Editorial Ciencia y Técnica. 1974;952.
Regalado Gabancho L, Ventosa Rodríguez I, Morejón Hernández R. Revisión histórica de los herbarios cubanos con énfasis en las series de especímenes. Rev del Jardín Botánico Nacional. 2008;29:101–38.
Cuba. Ministerio de Salud Pública. NRSP No. 309. Medicamentos de origen vegetal: droga cruda. Métodos de ensayos. La Habana: MINSAP; 1992.
Carballo C. Desinfección química de Pedilanthus tithymaloides L. Poit. Rev Cubana Plant Med. 2005;10(2):45–9.
Sandoval D, Suárez O. Estudio fitoquímico preliminar de detección de alcaloides y saponinas en plantas que crecen en Cuba. Rev Cubana Farm. 1990;24(2):288–96.
Rondina RVD, Coussio JD. Estudio fitoquímico de plantas medicinales argentinas (1). Rev Invest Agropec (Serie 2. Biología y Producción Vegetal). 1969;6(2):352–66.
Peña A, Torres E. Monografía de Productos Naturales. Granma: Universidad de Granma; 2006.
Yesilada E, Bedir E, Çalis I, Takaishi Z, Ohmoto Y. Effects of triterpene saponins from Astralagus species on in vitro cytokine release. J. Ethnopharmacol. 2005;96:71–7.
Semmar N, Tomofumi M, Mrabet Y, Lacaille-Dubois M-A. Two new acetylated tridesmosidic saponins from Astralagus armatus. Helv Chim. 2010;93:871–6.
Francis G, Kerem Z, Makkar HPS, Becker K. The biological action of saponins in animal systems: A review. British Journal of Nutrition. 2002;88(6):587–605.
Tomás ChG, Huamán MJ, Aguirre MR, Barrera TM. Extracción y clasificación de la saponina del Sapindus saponaria L., “boliche”. Rev Per Quím. 2010;13(2):36-9.
Macías FA, Guerra JO, Simonet AM, Pérez AJ, Nogueiras Clara. Characterization of three Saponins from a fraction using 1D DOSY as a solvent signal suppression tool. Agabrittonosides E–F. Furostane Saponins from Agave brittoniana Trel. spp. Brachypus. Magn. Reson. Chem. 2010;48:350–5.
Majinda RRT. Extraction and Isolation of Saponins. En: Satyajit S, Lutfun N. Natural Products Isolation, Methods in Molecular Biology. 3rd ed. UK: Humana Press Inc. 2012;864:415–6.
Naj JJ, Hahn JH, Chung S, Kim YI, Nah SY. Effects of ginsenosides active components of ginseng on capsaicin-induced pain-related behavior. Neuropharmacology. 2000;39:2180–84.
Damke E, Tsuzuki JK, Cortez DAG, Ferreira ICP, Bertoni TA, Batista MR, et al. In vivo activity of Sapindus saponaria against azole-susceptible and -resistant human vaginal Candida species. Complementary and Alternative Medicine. 2011;11:35–44.
Wagner H, Bladt S. Plant Drug Analysis: A Thin Layer Chromatography Atlas. 2nd ed. Berlin: Springer; 2001:3–353.
Cseke LJ, Kirakosyan A, Kaufman PB, Warber SL, Duke JA, Brielmann HL. Natural Products from Plants. 2nd ed. Boca Raton, FL: Taylor & Francis Group; 2006:30–6.
Shakeel-u-Rehman, Reehana K, Khursheed A, Raja AF, Shawl AS, Alam MS. Isolation, characterization and antibacterial activity studies of coumarins from Rhododendron lepidotum Wall. ex G. Don, Ericaceae. Braz. J. Pharmacogn. 2010;20(6):886–90.
Xiao-Tian L, Wei-Shuo F. Medicinal Chemistry of Bioactive Natural Products. Hoboken, New Jersey: John Wiley & Sons, Inc; 2006:325–51.
Smyth T, Ramachandran VN, Smyth WF. A study of the antimicrobial activity of selected naturally occurring and synthetic coumarins. Int J Antimicrob Ag. 2009;33:421–6.
Melliou E, Magiatis P, Mitaku S, Skaltosounis AL, Chinou E, Chinou I, et al. Natural and synthetic 2,2-dimethylpyranocoumarins with antibacterial activity. J Nat Prod. 2005;68:78–82.
Fraga CG. Plants Phenolics and Human Health: Biochemistry, Nutrition and Pharmacology. Hoboken, New Jersey: John Wiley & Sons Inc; 2010:51–3.
Santos-Buelga C, Gonzalez-Manzano S, Dueñas M, Gonzalez-Paramas AM. Extraction and Isolation of Phenolics Compounds. En: Satyajit S, Lutfun N. Natural Products Isolation, Methods in Molecular Biology. 3rd ed. UK: Humana Press Inc. 2012;864:438–9.