medigraphic.com
ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2015, Number 2

Next >>

Biotecnol Apl 2015; 32 (2)

Biodegradation in vitro of diesel bilge waters using a microbial native consortium isolated from Córdoba, Colombia

Mezquida R, Oviedo LE, Lara C

Language: English
References: 28
Page: 2201-2205
PDF size: 350.94 Kb.


ABSTRACT

The aim of this investigation was to evaluate the biodegradation of diesel bilge water using a microbial consortium native to the region of Cordoba, Colombia. Bacteria were isolated from a natural petroleum source, using mineral minimal (0.5 g/L KH2PO4; 1.4 g/L Na2HPO4; 0.6 g/L NH4NO3; 0.1 MgSO4·7H2O; 0.02 g/L CaCl2·2H2O; 0.03 g/L MnSO4·H2O; pH adjusted to 7.0) under conditions of bacterial growth (pH 7.0, 28 ± 2° C and shaking at 150 rpm). Microorganisms were tested for contaminant tolerance using different concentration of diesel bilge water: 3.0, 5.0, 7.5, 10.0, 12.5 and 15 %. Subsequently, a bioassay was performed in microcosms for 28 days to assess the biodegradation of diesel linters water to the highest concentration water tolerance found, i.e., 7.5 %. The bacterial consortium was grown up to 106 c.f.u./mL, the ratio of carbon/nitrogen adjusted to 100/5 and the biodegradation products were determined by gas chromatography, at the beginning and the end of the assay. There was found that some components were completely degraded (1-Himidazol-4-carbazamida), others around 70 % (1, 2, 3-triazol 4- carbohidrazida) and the rest at smaller rates. Among the species isolated with standard procedures and identified with the API 20 NE and API 20 E commercial kits were: Achromobacter denitrificans, Sphingomonas paucimobilis and Pseudomonas putida. Rhizobium radiobacter was also reported, as a new contribution to the list of bacterial species displaying biorremediation capacity.


REFERENCES

  1. Vieira P, Vieira R, Franca F, Cardoso V. Biodegradation of diesel oil and gasoline contaminated effluent employing intermittent aeration. J Hazard Mat. 2009;168:1366-72.

  2. Ministerio de Ambiente, Vivienda y Desarrollo Territorial. Política Ambiental para la Gestión Integral de los Residuos o Desechos Peligrosos. Decreto 4741. Bogotá: Dirección de Desarrollo Sostenible, Colombia; 2005.

  3. Nievas M, Commedatore M, Esteves J, Bucalá V. Effect of pH modification on bilge waste biodegradation by a native microbial community. Int Biodeter Biodegr. 2005;56:151-7.

  4. Paixão J, Nascimento IA, Pereira SA, Leite MB, Carvalho GC, Silveira JS, et al. Estimating the gasoline components and formulations toxicity to microalgae (Tetraselmis chuii) and oyster (Crassostrea rhizophorae) embryos: an approach to minimize environ mental pollution risk. Environmental. 2007;103:365-74.

  5. Barrios Y. Biorremediación: una herramienta para el saneamiento de ecosistemas marinos contaminados con petróleo. Biotecnol Apl. 2011;28:60-8.

  6. Fernandez-Luqueño F, ValenzuelaEncinas C, Marsch R, Martinez-Suarez C, Vazquez-Nunez E, Dendooven L. Microbial communities to mitigate contamination of PAHs in soil – possibilities and challenges: a review. Environ Sci Pollut Res. 2011;18:12-30.

  7. Gojgic-Cvijovic GD, Milic JS, Solevic TM, Beskoski VP, Ilic MV, Djokic LS, et al. Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study. Biodegradation. 2012;23:1-14.

  8. Pactao H, Suto K, Inoue C. Bacterial community dynamics during the preferential degradation of aromatic hydrocarbons by a microbial consortium. Int Biodeter Biodegr. 2012;74:109-15.

  9. Yaohui X, Mang L. Bioremediation of crude oil-contaminated soil: Comparison of different biostimulation and bioaugmentation treatments. J Hazard Mat. 2010;183:395-401.

  10. Grace Liu PW, Chang TC, Whang LM, Kao CH, Pan PT, Cheng SS .Bioremediation of petroleum hydrocarbon contaminated soil: Effects of strategies and microbial community shift. Int Biodeter Biodegr. 2011;65:1119-27.

  11. Taccaria M, Milanovic V, Comitini F, Casucci C, Ciani M. Effects of biostimulation and bioaugmentation on diesel removal and bacterial community. Int Biodeter Biodegr. 2012;66:39-46.

  12. Sayara T, Borràs E, Caminal G, Sarrà M, Sánchez A. Bioremediation of PAHscontaminated soil through composting: Influence of bioaugmentation and biostimulation on contaminant biodegradation. Int Biodeter Biodegr. 2011;65:859-65.

  13. Ministerio de Ambiente, Vivienda y Desarrollo Territorial. Política Ambiental para la Gestión Integral de los Residuos o Desechos Peligrosos. Decreto 3930. Bogotá: Dirección de Desarrollo Sostenible, Colombia; 2010.

  14. Holt SG, Kriey NR, Sneath PH, Staley JT, Williams ST. Bergey’s Manual of Determinative for Bacteriology. New York: Williams and Wilkins; 1998.

  15. Andrew Eaton, American Water Works Association, Water Environment Federation. Standard methods for the examination of water and wastewater. 21ed. Washington DC: APHA-AWWA-WEF; 2005.

  16. González PY, Alarcón A, FerreraCerrato R, Almaraz JJ, Martínez-Romero E, Cruz-Sánchez J, et al. Tolerance, growth and degradation of phenanthrene and benzo[a]pyrene by Rhizobium tropici CIAT 899 in liquid culture medium. Appl Soil Ecol. 2013;63:105-11.

  17. Choi HJ, Seo JY, Hwang SM, Lee YI, Jeong YK, Moon JY, et al. Isolation and characterization of BTEX tolerant and degrading Pseudomonas putida BCNU 106. Biotechnol Bioprocess Eng. 2013;18:1000-7.

  18. Cerqueira V, Hollenbach E, Maboni F, Vainstein M, Camargo F, Peralba M, et al. Biodegradation potential of oily sludge by pure and mixed bacterial. Bioresour Technol. 2011;102:1003-10.

  19. Benavides J, Quintero G, Guevara A, Jaimes D, Gutiérrez S, Miranda J. Biorremediación de suelos contaminados con hidrocarburos derivados del petróleo. NOVA – Publicaciones Científicas. 2006;4:1-116.

  20. Purohit H, Thangaraj K, Kapley A. Characterization of diverse Acinetobacter isolates for utilization of multiple aromatic compounds. Bioresour Technol. 2008;99:2488-94.

  21. Zhang X, Cheng S, Zhu C, Sun S. Microbial PAH-degradation in soil: Degradation pathways and contributing factors. Pedosphere. 2006;16:555-65.

  22. Yaohui X, Mang L. Bioremediation of crude oil-contaminated soil: Comparison of different biostimulation and bioaugmentation treatments. J Hazard Mat. 2010;183:395-401.

  23. García E, Roldán F, Garzón L. Evaluación de la bioestimulación (nutrientes) en suelos contaminados con hidrocarburos utilizando respirometría. Acta Biol Colomb. 2011;16:195-208.

  24. Janbandhu A, Fulekar MH. Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment. J Hazard Mat. 2011;187:333-40.

  25. Richard J, Vogel T. Characterization of a soil bacterial consortium capable of degrading diesel fuel. Int Biodeter Biodegr, 1999;44:93-100.

  26. Nievas M, Commedatore M, Esteves J, Bucalá V. Biodegradation pattern of hydrocarbons from a fuel oil type complex residue by an emulsifier-producing microbial consortium. J Hazard Mat. 2008;154:96-104

  27. Lakha S, Miller M, Campbell RG,Schneider K, Elahimanesh P, et al. Trevors JT. Microbial gene expression in soil: methods, applications and challenges. J Microbiol Methods. 2002;63(1):1-19.

  28. Marchal R, Penet S, Serena F, Vandecasteele JP. Gasoline and diesel oil biodegradation. Oil Gas Sci Technol. 2003;58:441-8.




2020     |     www.medigraphic.com