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2015, Number 1

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Rev Cubana Plant Med 2015; 20 (1)

Fitochemistry evaluation of leaves of Excoecaria lucida Sw. (Aité) (Euphorbiaceae) and isolation e identification of a hemiterpenoid

Ochoa PA, Escalona AJC, Fechine TJ, Sobral SM

Language: Spanish
References: 27
Page: 117-130
PDF size: 168.34 Kb.


ABSTRACT

Introduction: Excoecaria lucida Sw. (Aité), it is a species that grow in Cuba and at the Caribbean region. It has been scientifically quite a little studied, in spite of the fact that the population utilizes it due to their medicinal properties as antiasthmatic, antimicrobial, and for the toothache treatment.
Objectives: to determine the chemical composition of leaves, as well as the isolation and identification of their secondary metabolites.
Methods: dried and milled leaves were macerated 72 hours with ethanol 95 %, repeating the procedure in 4 occasions. Total extract was determined in his chemical qualitative composition and afterwards it was fragmented with solvents. The phase with greater yield was studied by High Performance Liquid Chromatography and isolated the main substance, which was characterized by infrared and Nuclear Magnetic Resonance spectroscopy: uni-dimensional 1H and 13C at 500 MHz and bi-dimensional HMQC, HMBC, COSY and NOESY.
Results: the identification of Alkaloids, triterpenes and steroids, quinone, flavonoids, coumarins, sesqiterpenic lactones, tannins and phenols were accomplished. From the greater yield phase (butanol= 23.48 g) was isolated and identified the main compound, a hemiterpenoid.
Conclusions: the identified secondary metabolites are in coincidence with other reports related to the genera. For first time the hemiterpene (2E)-2-methyl-2- buten-1,4-di-hydroxyl-1-O-β-D-glucopyranoside is informed in this genera and specie.


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