medigraphic.com
ISSN 1028-4796 (Print)

2015, Number 1

<< Back Next >>

Rev Cubana Plant Med 2015; 20 (1)

Antioxidant and cytotoxic activity of extracts of Pilea dauciodora Wedd (Urticaceae)

Garro A, Cardona W, Rojano B, Robledo SM, Alzate F

Language: Spanish
References: 34
Page: 88-97
PDF size: 163.09 Kb.


ABSTRACT

Introduction: the free radical production due to oxidative stress, causing the oxidation of lipids, proteins, DNA and enzymes, responsibles for cell tissue damage. Some species of the Urticaceae family exhibit a wide spectrum of biological activities, which makes striking exploring the antioxidant property for members of the genus Pilea Lindl., as an alternative in the search for new natural antioxidants.
Objective: to evaluate the cytotoxic and antioxidant activity of alcoholic and nonalcoholic extracts of Pilea dauciodora Wedd.
Methods: after drying the plant material, leaves were subjected to a process by soxhlet extraction with solvents of different polarities (dichloromethane, ethyl acetate and ethanol); a similar procedure was used to prepare the ethanol extract. The solutions obtained were concentrated under reduced pressure and were assayed for antioxidant capacity by different methods and cytotoxicity in U-937 cells.
Results: the ethanol extract of the stems and the ethyl acetate extract of the leaves showed the best reducing capacity and lower cytotoxicity, which makes promising these extracts in the search of new antioxidant compounds.
Conclusion: Pilea dauciodora Wedd. shows reductive property, which represents high potential as a natural source of antioxidants for using in pharmaceutical and food industry.


REFERENCES

  1. Rojano B, Saez J, Schinella G, Quijano J, Vélez E, Gil A, et al. Experimental and theoretical determination of the antioxidant properties of isoespintanol (2-isopropyl- 3,6-dimethoxy-5-methylphenol). J Mol Struc. 2008;(877):1-6.

  2. Buenger J, Ackermann H, Jentzsch A, Mehling A, et al. An interlaboratory comparison of methods used to assess antioxidant potentials. Int J Cosmetic Sci. 2006;(28):135-45.

  3. Atawodi S.E. Antioxidant potential of African medicinal plants. Afr J Biotechnol. 2005;(4):128-33.

  4. Botterweck AAM, Verhagen H, Goldbohm RA, Kleinjans J, Van den brandt PA. Intake of Butylated Hydroxyanisole and Butylated Hydroxytoluene and Stomach Cancer Risk: Results from Analyses in the Netherlands Cohort Study. Food Chem Toxicol. 2000;(38):599-605.

  5. Gaviria C, Ochoa C, Sánchez N, Medina C, Lobo M, et al. Actividad antioxidante e inhibición de la peroxidación lipídica de extractos de frutos de mortiño (Vaccinium meridionale SW). Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 2009;(6):519-28.

  6. Rojano B, Gaviria C, Gil M, Saez J, Schinella G, Tournier H. Actividad antioxidante del isoespintanol en diferentes medios. Vitae. 2008;(15):173-81.

  7. Pokorny J. Natural antioxidants for food use. Trends Food Sci Technol. 1991;(9):223-7.

  8. Chen JR, Monro AK. In Flora of China; Science Press: Beijing. 2003;(5):92-120.

  9. Chinese Medicinal Material Company Resource Compendium for Chinese Traditional Medicine; Science Press: Beijing, 1994. pp. 201-5.

  10. Facey PC, Pascoe KO, Porter RB, Jones AD. Investigation of plants used in Jamaican folk medicine for anti-bacterial activity. J Pharm Pharmacol. 1999;(51):1455-60.

  11. Modarresi CA, Ibrahim D, Fariza S. Antioxidant, antimicrobial activity and toxicity test of Pilea microphylla. Int J Microbiol. 2010;(2010):1-6.

  12. Prabhakar KR, Veerapur VP, Bansa lP, Parihar VK, Reddy KM, Bhagath KP, et al. Antioxidant and radioprotective effect of the active fraction of Pilea microphylla (L.) Liebm. ethanolic extract. Chem Biol Interact. 2007;(165):22-32.

  13. Taylor VM, Cedeño DL, Muñoz DL, Jones MA, Lash TD, Young AM, et al. In vitro and vivo studies of the utility of dimethyl and diethyl carbaporphyrin ketals in treatment of cutaneous leishmaniasis. Antimicrob Agents Chemother. 2011;55:4755-64.

  14. Finney JD. Probit Analysis. 3rd ed. United Kingdom: Cambridge University Press, Cambridge; 1971.

  15. Dewanto V, Wu X, Adom KK, Liu RI. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem. 2002;(50):3010-14.

  16. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem. 1996;(239):70-6.

  17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med. 1999;(26):1231-7.

  18. Peyrat-Maillard MN, Bonnely S, Berset C. Determination of the antioxidant activity of phenolic compounds by coulometric detection. Talanta. 2000;(51):709-16.

  19. Jimenez N, Londoño J, Arango G. Actividad captadora de radicales libres y citotoxicidad de plantas colombianas de la familia Annonaceae. Acta Farm Bonaerense. 2005;(24):337-42.

  20. Peteros N, Uy M. Antioxidant and cytotoxic activities and phytochemical screening of four Philippine medicinal plants. J Med Plants Res. 2010;(4):407-14.

  21. Rekka E, Kourounakis PN. Effect of hydroxyethylrutenosides and related compounds on lipid peroxidation and free radical scavenging activity. Some structural aspects. J Pharm Pharmacol. 1991;(43):486-91.

  22. Zhu N, Wang M, Wei G-J, Lin J-K, Yang CS, Ho CT. Identification of reaction products of (-)-epigallocatechin, (-)-epigallocatechingallate and pyrogallol with 2,2- diphenyl-1-picrylhydrazyl radical. Food Chem. 2001;(73):345-49.

  23. Bondent V, Brand-Williams W, Bereset C. Kinetics and mechanism of antioxidant activity using the DPPH free radical methods. LWT – Food Sci Technol. 1997;(30):609-15.

  24. Chahardehi AM, Ibrahim D, Sulaiman SF. Antioxidant activity and total phenolic content of some medicinal plants in Urticaceae family. J Appl Biol Scien. 2009;(3):27-31.

  25. Benzie IF, Strain JJ. Ferric reducing antioxidant power assay; direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration methods. Enzymol. 1999;(299):15-27.

  26. Gordon MH. The mechanism of antioxidant action in-vitro. In: Hudson BJF. Editors. Food antioxidants. London: Elsevier Applied Science; 1990. p. 1-18.

  27. Puertas M, Chabala L, Rojano B, Sáez J. Capacidad antioxidante in vitro de fracciones de hojas de Piper peltatum L. Rev Cubana Plant Med. 2009;(14):1-11.

  28. Cardona W, Robledo S, Rojano B, Alzate F, Muñoz D, Saez J. Actividad leishmanicida y antioxidante de extractos de Piper daniel-gonzalezii Trel. (Piperaceae). Rev Cubana Plant Med. 2013;(18):268-77.

  29. Wolfenden BS, Willson RL. Radical-cations as reference chromogens in kinetic studies of one-electron transfer reactions: pulse radiolysis studies of 2,2 9-azinobis- (3-ethylbenzthiazoline-6-sulphonate). J Chem Soc Perkin Trans. 1982;(2):805-12.

  30. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996;(20):933-56.

  31. Salah N, Miller NJ, Paganga G, Tijburg L, Rice-Evans CA. Polyphenolic flavonols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Arch Biochem Biophys. 1995;(322):339-46.

  32. Roberta RE, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med. 1999;(26):1231-37.

  33. Galato D, Ckless K, Susin MF, Giacomelli C, Ribeiro do Valle RM, Spinelli A. Antioxidant capacity of phenolic and related compounds: correlation among electrochemical, visible spectroscopy methods and structure-antioxidant activity. Redox Report. 2001;(6):243-50.

  34. Naczk M, Shaidi F. Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. J Pharm Biomed Anal. 2006;(41):1523-42.




2020     |     www.medigraphic.com