Alterations in dog ovaries actively immunized with pig oocyte proteins

Héctor Serrano; María Dolores García-Suárez

2001, Number 3

<< Back Next >>

Vet Mex 2001; 32 (3)

Alterations in dog ovaries actively immunized with pig oocyte proteins

Serrano H, García-Suárez MD

Full text

How to cite this article

Language: English/Spanish
References: 22
Page: 221-224
PDF size: 35.08 Kb.

ABSTRACT

Feral dog population control is not an easy task in highly populated cities, even when there are high cost sterilization campaigns. Mexico City’s street dog population increases constantly, invasive sterilization techniques are out of the regular family budget, and an alternative strategy to control this population is needed. Since the early 70´s, the acellular egg coat named the zona pellucida has been considered as a target for fertility regulation in human and animal populations. The goal of the present work was to analyze the alterations in the ovarian histology of female dogs actively immunized with porcine ZP3 protein. Results obtained show that active immunization induces a significant reduction in fertility as indicated by the lower amount of first and second degree follicles, and an increase in leukocyte infiltration in response to the protein inoculation.

REFERENCES

Ambriz-García D, Hernández-Hernández A, Juárez-Jiménez J. Encuesta de población y condiciones de los cánidos en Amecameca, Estado de México. Memorias del IV Encuentro de Intercambio para la Investigación Agropecuaria en Biología de la Reproducción; 1994 octubre 6-7; México (DF). México (DF): Universidad Autónoma Metropolitana, 1994:55-60.
2. Olson PN, Moulton C. Pet (dog and cat) overpopulation in the United States. J Reprod Fertil 1993;47:433-438.
3. Wassarman PM, Albertini DA. The mammalian ovum. In: Knobil E, Neil JD, editors. The physiology of reproduction. New York: Raven Press, 1994:79-122.
4. Hedrick JL, Wardrip NJ. Isolation of the zona pellucida and purification of its glycoprotein families from pig oocytes. Anal Biochem 1986;157:63-70.
5. Karp DR, Atkinson J, Schreffler DC. Genetic variation in glycosylation of the fourth component of murine complement. Association with hemolytic activity. J Biol Chem 1982;257:7330-7335.
6. Smith K, Krohn RI, Hermanson GT, Gartner FH, Provenzano MD, Fujimoto EK., et al. Measurement of protein using bicinchoninic acid. Anal Biochem 1985;150:76-85.
7. Barber MR, Fayrer-Hosken RA. Evaluation of somatic and reproductive immunotoxic effects of the porcine zona pellucida vaccination. J Exp Zool 2000;286:641-646.
8. Canavos GC. Probabilidad y estadística. Aplicaciones y métodos. México (DF): McGrawHill-Interamericana, 1988.
9. Artois M. Managing problem wildlife in the “Old World”: a veterinary perspective. Reprod Fertil Dev 1997;9:17-25.
10. Gwatkin RBL. Anti-fertility vaccine comparising solubilized zona pellucida and anti-serum. Washington (DC) Patent 4996297, November 16th 1976.
11. Mahi-Brown CA, Huang TTF, Yanagimachi R. Infertility in bitches induced by active immunization with porcine zona pellucida. J Exp Zool 1982;222:89-95.
12. Mahi-Brown CA, Yanagimachi R, Hoffman JC, Huang TTF. Fertility control in the bitch by active immunization with porcine zona pellucida. Use of different adjuvants and patterns of estradiol and progesterone levels in estrous cycles. Biol Reprod 1988;32:761-772.
13. Henderson CJ, Hulme MJ, Aitken RJ. Contraceptive potential of antibodies to the zona pellucida. J Reprod Fertil 1988;83:325-343.
14. Grimes S, Benjamini E, Gevas P. Method for contraception by immunization against the zona pellucida. Washington (DC): patent 4795634, January 3rd. 1989.
15. Skinner SM, Mills T, Kirck HJ, Dunbar BS. Immunization with zona pellucida proteins result in abnormal ovarian follicular differentiation and inhibition of gonadotropin-induced steroid secretion. Endocrinology 1984;115:2418-2432.
16. Kirkpatrick JF, Liu MK, Keiper R. Long-term effects of porcine zonae pellucidae immunocontraception on ovarian function in feral horses (Equus caballus). J Reprod Fertil 1992;94:437-444.
17. Dunbar BS. Recombinantly expressed rabbit zona pellucida polypeptides. patent 4996297, February 16. Washington, DC, U.S.A., 1991.
18. Serrano H, Mendoza-Perez, JA, Vázquez-Granados JA, Casas E, Betancourt M. cDNA library expressing pig ovarian zona pellucida genes. J Cell Biol 1991;115:462ª.
19. Yurewicz EC, Hibler H, Fontenot GK, Sacco AG, Harris J. Nucleotide sequence of cDNA encoding ZP3a a sperm-binding glycoprotein from zona pellucida of pig oocyte. Biochem Biophys Acta 1993;1174:211-214.
20. Schwoebel E, Prasad S, Timmons TM, Cook R, Kimura H, Niu EM, et al. Isolation and characterization of a full-length cDNA encoding the 55-kDA rabbit zona pellucida protein. J Biol Chem 1991;266:7214-7219.
21. Sun W, Lou Y-H, Dean J, Tung KSK. Contraceptive peptide vaccine targeting sulfated glycoprotein ZP2 of the mouse zona pellucida. Biol Reprod 1999;60:900-907.
22. Kerr PJ, Jackson RJ, Robinson AJ, Swan J, Silvers L, French N, et al. Infertility in female rabbits (Oryctolagus cuniculus) alloimmunized with the rabbit zona pellucida protein ZPb either as a purified recombinant protein or expressed by recombinant myxoma virus. Biol Reprod 1999;61:606-613.