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2014, Number 4

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Rev Cubana Plant Med 2014; 19 (4)

Antimicrobial activity and phytochemical characterization of hydroalcoholic extracts of Passiflora cincinnata Mast. (maracujá-do-mato)

de Albuquerque SAL, Santiago LIC, de Araújo DG, Rolim OL, Oliveira BPBMA, de Carvalho SD, Melo CHD, Sabino AR, Figueiredo LN, Martins CJG, Alencar MIR, Kerntopf MR

Language: Portugués
References: 32
Page: 319-328
PDF size: 117.44 Kb.


ABSTRACT

Introduction: Passiflora cincinnata Mast. (maracujá do mato), of the family Passifloraceae, is widely used in herbal medicine. This plant is characterized by its resistance to diseases and pests, longevity, ability to adapt to adverse weather conditions, and a high concentration of chemical components used by the pharmaceutical industry and associated to an almost unexplored range of potential uses.
Objective: characterize the phytochemical composition and evaluate the antimicrobial and modulatory activity of hydroalcoholic extracts obtained from dry aerial parts (leaves, stem, peel, pulp and seeds) of Passiflora cincinnata Mast.
Methods: the extracts were prepared by maceration in alcohol solution. Minimum inhibitory concentration tests were performed by broth dilution against Pseudomonas aeruginosa strain ATCC15442, whereas modulatory activity of antibiotics was determined in relation to Pseudomonas aeruginosa strain 04, clinically isolated by sub-inhibitory concentration. The antibiotics tested were amikacin, gentamicin, ampicillin, benzylpenicillin potassium and oxacillin. Photochemical identification was determined by changes in color and the formation of precipitates.
Results: the extracts did not show any antimicrobial activity of clinical relevance, their minimum inhibitory concentration always being either greater than or equal to 1 024 µg/mL. The pulp and peel showed synergism with antibiotics, altering the phenotype of P. aeruginosa, which was transformed from resistant to sensitive when amikacin was used. The pulp extract potentiated the effect of the beta-lactams benzylpenicillin potassium and oxacillin. The stems, seeds and leaves did not show any modulatory activity. Phytochemical exploration identified the following secondary metabolites: tannins, flobacenes, flavones, flavonols, xanthones, chalcones, aurones, flavanones, leucoanthocyanidin, catechins and alkaloids.
Conclusions: the combined effect of hydroalcoholic extracts of P. cincinnata peel and pulp and antibiotics offers new therapeutic alternatives for the development of a pharmaceutical product based on multiple drugs.


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