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2014, Number 4

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Rev Cubana Estomatol 2014; 51 (4)

Low-level laser irradiation on Candida strains: an in vitro study

Lira De Souza SREA, Medeiros RCA, Rodrigues LFI, Dantas MAC, Chaves VCMH, Queiroga de Castro GD

Language: Portugués
References: 22
Page: 358-365
PDF size: 102.44 Kb.


ABSTRACT

Objective: to evaluate the effect of specific parameters of low-level laser irradiation on strains of Candida albicans (ATCC 18804), Candida krusei (ATCC 34135) and Candida tropicalis (ATCC 13803).
Methods: the inocula of the three Candida species (1.5 x 106 microorganisms/ml) were irradiated with a gallium-arsenide (GaAs) infrared laser device (Twinflex Evolution, MMO Electronic Equipment, 660 nm, 0.5 nW) at doses (J/cm2): 1.2 (10 sec), 3.7 (30 sec), 7.5 (1min) and 15 (2 min). Following irradiation, the inocula were grown on Petri dishes containing Sabouraud Dextrose culture medium and then incubated in bacteriological incubator at 37 °C. After 48 hours, it was quantified the number of colony-forming units (CFU) and data were statistically analyzed using Friedman’s and Wilcoxon’s tests (α = 0.05). All tests were performed in duplicate.
Results: the median values (Q25 - Q75) gathered in the quantification of the strains after laser irradiation at doses (J/cm2) 1.2, 3.7, 7.5 and 15 were, respectively: 35.23 (9,15-47,64); 6,79 (1,45-6,87); 5,32 (1,39-8,15); 6.10 (1,18-11,86) and 5.13 (0,99-6,25). These results were found to show statistically significant differences according to the dose administered (p ‹ 0.05). Nevertheless, it was not possible to identify in the post-hoc tests which group(s) showed significant difference.
Conclusion: low-intensity laser therapy showed inhibitory effect on Candida strains, and such activity was altered according to the irradiated dose.


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