Ramírez LJ, Osorio SD

Language: English/Spanish
References: 23
Page: 335-341
PDF size: 186.61 Kb.

ABSTRACT

The aim of the present study was to inform about the microscopic lesions caused by Eustrongylides sp in the skeletal muscle of frogs from Lake Cuitzeo, located in Michoacan, Mexico, given that it is considered to be a potentially zoonotic parasite. 146 frogs, from four species were studied: 80 from Rana megapoda, 43 from Rana dunni, 16 from Rana catesbiana and seven from Rana verlandieri. All were euthanised with an intraperitoneal pentobarbital overdose. The total body weight, weight of the legs, gender and total length were registered. An external and internal examination was then carried out, using a stereoscopic microscope, and the Eustrongylides sp nematodes were removed using fine brushes. These were fixed first in 70% ethyl alcohol and then in Amman lactophenol to facilitate their observation and taxonomic identification under the microscope. Necropsies were performed on the 146 total specimens from the four species, finding 10 parasitic larval forms in the leg muscles of the Rana megapoda specimens. Therefore, skeletal muscle fragments measuring 0.1 × 0.2 cm were extracted and fixed in 10% buffered formalin with a pH of 7.2. These were processed with the routine hematoxylin-eosin technique for their inspection under the light microscope. Multiples focal hemorrhages, a moderate quantity of heterophils between muscle fibers and a few round nematodes with a thin cuticle, muscle and pharynx, were observed. It is important to note that more studies must be carried out on these parasites, given that the frogs are of commercial importance for human consumption in the area, and can cause zoonosis and economic loss.

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