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Órgano Oficial de la Asociación Mexicana de Hepatología

2014, Number 4

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Ann Hepatol 2014; 13 (4)

Deep sequencing analysis of microRNA expression in porcine serum-induced hepatic fibrosis rats

Ge S, Wang X, Xie J, Yi X, Liu F

Language: English
References: 46
Page: 439-449
PDF size: 514.87 Kb.


ABSTRACT

Aim. Recent studies have suggested miRNA dysregulation in liver tissue mediates the pathogenesis of various liver diseases especially liver fibrosis, but the microRNA changes during PS-induced hepatic fibrosis are still unknown. The purpose of this study was to screen the miRNA differences in rat liver fibrosis model and clarify the relationship of miRNAs with the development of PS-induced liver fibrosis. Material and methods. Two fibrotic and two normal liver tissues from 20 Sprague–Dawley rats were collected and sequenced. MiRNA profiling results and fibrosis-related genes were validated by quantitative real-time polymerase chain reaction (qRT-PCR) and bioinformatics was used to predict miRNA targets. Results. In total, 48 miRNAs were detected to be aberrantly expressed in fibrosis tissue compared to normal tissue. Further functional analysis of the deregulated miRNA targets revealed the miRNAs are involved in several biological functions and pathways. In addition, the expression level of miR-27a and miR-146b and fibrosis-related genes were significantly up-regulated by using qRT-PCR in fibrotic liver tissues when compared to the normal liver tissues. Conclusion. PS-induced hepatic fibrosis results in up-regulation of the miR-27a and miR-146b in liver tissues, suggestingmiR-27a and miR-146b would be associated with the development of PS-induced liver fibrosis and be potential therapeutic targets during hepatic fibrosis.


REFERENCES

  1. Christopher JP, Motoki T,Richard AR. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol 2007; 22: S79-S84.

  2. Elisabetta M, Joseph G, Natalia N. Molecular pathogenesis of hepatic fibrosis and current therapeutic approaches. Chemico-biological interactions 2011; 193: 225-31.

  3. Friedman SL. Hepatic fibrosis-overview. Toxicology 2008; 254: 120-9.

  4. Yoshiki M, Hidenori T, Masami T, Masahiko K, Yoshinori H, Fumihiko M, Atsushi T, et al. The progression of liver fibrosis is related with overexpression of the miR-199 and 200 families. PLoS One 2011; 6: 16081.

  5. Soyer MT, Ceballos R, Aldrete JS. Reversibility of severe hepatic damage caused by jejunoileal bypass after re-establishment of normal intestinal continuity. Surgery 1976; 79: 601.

  6. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004; 116: 281-97.

  7. Alvarez-Garcia I, Miska EA. MicroRNA functions in animal development and human disease. Development 2005; 132: 4653-62.

  8. Miska EA. How microRNAs control cell division, differentiation and death. Current opinion in genetics & development 2005; 15: 563-568.

  9. Robert EL, Elisabeth SH, Andreas P, Robert P, Morten L,Martin EM, Sakari K, et al. Therapeutic silencing of microRNA- 122 in primates with chronic hepatitis C virus infection. Science 2010; 327: 198-201.

  10. Roderburg C, Luedde T. The role of miRNAs in animal models of liver fibrosis. Drug Discovery Today: Disease Models 2012; 363: 1-6.

  11. Janaiah K, Raghu RC, Kathryn AO, Erik AW, Chrystal LM, Hun-Way H, Tsung-Cheng C. et al. Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Cell 2009; 137: 1005-17.

  12. Ashley ML, Nury MS, Tracy LW, Sriparna G, Ting L, Iain HM, Mark WR, et al. Inhibitory effects of microRNA 19b in hepatic stellate cell©mediated fibrogenesis. Hepatology 2012; 56: 300-10.

  13. Koziel M. The immunopathogenesis of HBV infection. Antiviral therapy 1998; 3: 13.

  14. Wei-Qing L, Chao C, Mi-Die X, Jia G, Yi-Ming L, Qing-Mei X, Hui-Min L, et al. The rno©miR©34 family is upregulated and targets ACSL1 in dimethylnitrosamine©induced hepatic fibrosis in rats. FEBS Journal 2011; 278: 1522-32.

  15. Christoph R, Gerd-Willem U, Kira B, Mihael V, Henning Z, Sabine Schmidt, Jörn J,et al. Micro©RNA profiling reveals a role for miR©29 in human and murine liver fibrosis. Hepatology 2011; 53: 209-18.

  16. Hasegawa-Baba Y, Doi K. Changes in TIMP-1 and -2 expression in the early stage of porcine serum-induced liver fibrosis in rats. Exp Toxicol Pathol 2011; 63: 357-61.

  17. Osuna-Martínez U, Reyes-Esparza JA, L-Petricevich V, Hernandez-Pando R, Rodriguez-Fragoso L. Protective effect of thymic humoral factor on porcine serum-induced hepatic fibrosis and liver damage in Wistar rats. Ann Hepatol 2011; 10: 540.

  18. Jan K, Nikolaus R, Ravi B, Kallanthottathil GR, Thomas T, Muthiah M, Markus S. Silencing of microRNAs in vivo with ‘antagomirs’. Nature 2005;438: 685-9.

  19. Christine E, Scott D, Susan FM, Xing XY, Sanjay KP, Michael P, Lynnetta W, et al. miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting. Cell metabolism 2006; 3: 87-98.

  20. Catherine LJ, MinKyung Y, Alissa ML, Stanley ML, Peter S. Modulation of hepatitis C virus RNA abundance by a liverspecific MicroRNA. Science 2005; 309: 1577-81.

  21. Shimizu I, Mizobuchi Y, Yasuda M, Shiba M, Y-R M, Horie T, Liu F, et al. Inhibitory effect of oestradiol on activation of rat hepatic stellate cells in vivo and in vitro. Gut 1999; 44: 127-36.

  22. Osuna-Martínez U, Reyes-Esparza JA, L-Petricevich V, Hernandez-Pando R, Rodriguez-Fragoso L. Protective effect of Thymic Humoral Factor on porcine serum induced hepatic fibrosis and liver dam age in Wistar rats. Ann Hepatol 2011; 10: 540-51.

  23. Ruiqiang L, Chang Y, Yingrui L, Tak-Wah L, Siu-Ming Y, Karsten K, Jun W. SOAP2: an improved ultrafast tool for short read alignment. Bioinformatics 2009; 25: 1966-7.

  24. Piotr JB, Piero C, Carsten OD, Jun K, Yoshihide H, Werner VB, Christian B, et al. Methods for analyzing deep sequencing expression data: constructing the human and mouse promoterome with deepCAGE data. Genome Biol 2009; 10: 79.

  25. Audic S, Claverie JM. The significance of digital gene expression profiles. Genome research 1997;7: 986-95.

  26. Zhenbiao X, Maoshan C, Zhonggan R, Nian Z, Hanmei X, Xiao L, Geng T, et al., Deep sequencing identifies regulated small RNAs in Dugesia japonica. Molecular biology reports 2013; 1-7.

  27. Harsh D, Carsten S,Priyanka P, Norbert G. miRWalk–database: prediction of possible miRNA binding sites by “walking” the genes of three genomes. J Biomed Informatics 2011; 44: 839-47.

  28. Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 2005; 120: 15-20.

  29. Anton JE, Bino J, Ulrike G, Thomas T, Chris S, Debora SM. MicroRNA targets in Drosophila. Genome biology 2004; 5: R1.

  30. Ana C, Stefan G, Juan MG, Javier T, Manuel T, Montserrat R. Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 2005; 21: 3674-6.

  31. Jia Y, Lin F, Hongkun Z, Yong Z, Jie C, Zengjin Z, Jing W, et al. WEGO: a web tool for plotting GO annotations. Nucleic acids research 2006; 34: W293-W297.

  32. Glynn DJ, Brad TS, Douglas AH, Jun Y, Wei G, H CL, Richard AL. DAVID: database for annotation, visualization, and integrated discovery. Genome Biol 2003; 4: 3.

  33. Xiaoyu F, Deming T, Zhouhua H, Zhiliang H, Guozhen L. miR-338-3p Is Down-Regulated by Hepatitis B Virus X and Inhibits Cell Proliferation by Targeting the 3’-UTR Region of CyclinD1. International Journal of molecular sciences 2012; 13: 8514-39.

  34. Angela D, Jan P, Karen K, Donna C, Pranoti M, Arumugam V, Gyongyi S. MicroRNA Expression Profile in Lieber©DeCarli Diet©Induced Alcoholic and Methionine Choline Deficient Diet©Induced Nonalcoholic Steatohepatitis Models in Mice. Alcoholism: Clinical and Experimental Research 2009; 33: 1704-10.

  35. Can-Jie G, Qin P, Tao C, Bo J, Guang-Yu C, Ding-Guo L. Changes in microRNAs associated with hepatic stellate cell activation status identify signaling pathways. FEBS Journal 2009; 276: 5163-76.

  36. Can-Jie G, Qin P, Ding-Guo L, Hua S, Bo-Wei L. miR-15b and miR-16 are implicated in activation of the rat hepatic stellate cell: An essential role for apoptosis. J Hepatol 2009; 50: 766-78.

  37. Can-Jie G, Qin P,Bo J, Guang-Yu C, Ding-Guo L. Effects of upregulated expression of microRNA-16 on biological properties of culture-activated hepatic stellate cells. Apoptosis 2009; 14: 1331-40.

  38. Jianjian Z, Zhuo L, Peihong D, Zhongqiu L, Shenmeng G, Xiaoqian C, Cunzao W, et al. Activation of hepatic stellate cells is suppressed by microRNA-150. International Journal of Molecular Medicine 2013; 32: 17.

  39. Masashi I, Tomohiro O, Masaru E, Hiroyuki M, Katsutoshi Y, Kazuo I, Norifumi K. Induction of microRNA-214-5p in human and rodent liver fibrosis. Fibrogenesis Tissue Repair 2012; 5: 12.

  40. Juling J, Jinsheng Z, Guangcun H, Jin Q, Xueqing W, Shuang M. Over-expressed microRNA-27a and 27b influence fat accumulation and cell proliferation during rat hepatic stellate cell activation. FEBS letters 2009; 583: 759-66.

  41. Jiang X, Ning Q, Wang J. Angiotensin II induced differentially expressed microRNAs in adult rat cardiac fibroblasts. J Physiol Sciences 2013; 63: 31-8.

  42. Yanyan L, Ying W, Lin Y, Cuiyun Sc, Degang C, Shizhu Y, Qian W, et al. miR-146b-5p inhibits glioma migration and invasion by targeting MMP16. Cancer letters 2013; 339: 260-9.

  43. Richard F, Roland R, Thuy PL, Alexandra S, Ulrich W, Hans PD, Dieter H. Intercellular communication via gap junctions in activated rat hepatic stellate cells. Gastroenterology 2005; 128: 433-48.

  44. Lim M, Maubach G, Zhuo L. TGF-beta1 down-regulates connexin 43 expression and gap junction intercellular communication in rat hepatic stellate cells. Eur J Cell Biol 2009; 88: 719.

  45. Emma AK, Paulo RA, Gaurav A, Jin Y, Michael HN, Jonathan AD. Molecular basis for calcium signaling in hepatic stellate cells. American Journal of Physiology-Gastrointestinal and Liver Physiology 2007; 292: G975-G982.

  46. An P, Tian Y, Dai J, Chen M, Luo H. Ca2+/Calmodulin-Dependent Protein Kinase II Mediates Platelet-Derived Growth Factor-Induced Human Hepatic Stellate Cell Proliferation. Digestive diseases and sciences 2012; 57: 935-42.




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