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2002, Number 2

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Vet Mex 2002; 33 (2)

A non-invasive methods to record vital signs in rats

Flores CPL, Infante VO, Sánchez TG, Martínez MR, Rodríguez RG

Language: English/Spanish
References: 13
Page: 179-187
PDF size: 312.43 Kb.


ABSTRACT

Equipment designed to record plethysmographic pulse (PP), systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), respiratory rate (RR) and the electrocardiogram (ECG) in rats by a non-invasive method is described in this work. The system was adapted to a special commercial acrylic cage and modified with a flat acrylic platform embedded with four separate electrodes to take the monopolar or differential ECG, from which the heart rate is obtained. The PP was registered using an original pincer which can be adapted to the rat’s tail and which holds two photo-emitter diodes and one photodetector using an inflatable chamber strapped around the tail and blood pressure and pulse oscillations are obtained. A photodetector unit placed on the cage and directed towards the dorsal thorax of the rat was used to record the respiratory rate. After amplification and filtration, all signals passed through an analog to digital converter and then to a personal computer programmed to present, process and save the data. This device has been tested in 22 rats, and the following mean parameters have been found: SBP = 113 mmHg, DBP = 91 mmHg, HR = 352 beats per minute, and RR = 105 breaths per minute; values which are in accordance with those that have been previously published.


REFERENCES

  1. Fox JG. Laboratory animal medicine. New York: Academic Press, 1984.

  2. 2. Bunag RD. Measuring blood pressure in laboratory animals. In: O’Brien E, O’Malley K, editors. Handbook of hypertension, blood pressure measurement. Vol. 14. Amsterdam, The Netherlands: Elsevier Science, 1991.

  3. 3. Guide for the human use of animals in research. Orlando (Fl): Niehs Handbook for Investigators & Technicians, 1988.

  4. 4. Geddes LA. The direct and indirect measurement of blood pressure. Chicago (Ill): Year Book Medical Publishers, 1979.

  5. 5. Infante O, Sánchez G, González M, Porter F. Medición del retardo entre la onda Q del electrocardiograma y el pie de ascenso del pulso sanguíneo; reporte preliminar. Rev Mex Ing Bioméd 1994;15:124-126.

  6. 6. Infante O, Valenzuela F, Polo S. Algoritmo que utiliza la segunda derivada para identificar el complejo QRS en tiempo real. Rev Mex Ing Bioméd 1992;13:23-32.

  7. 7. Rodríguez G, Infante O, Valenzuela F, Espinosa L, González C. Sistema de adquisición de señales fisiológicas. Rev Mex Ing Bioméd 1988;9:25-35.

  8. 8. Neuman RM. Biopotential amplifiers. In: Webster JB, editor. Blood pressure and sound. Medical instrumentation application and design. 2nd ed. Boston (Ma): Houghton Mifflin, 1992.

  9. 9. Infante O, Rodríguez G, Pérez J, Espinoza L, Valenzuela F, Rojas M. Terminal de electrocardiografía. Rev Mex Ing Bioméd 1988;9:87-95.

  10. González M, Infante O, Sánchez G, Flores P. Medición experimental no invasiva de la presión arterial sistémica y de la transmisión del pulso. Reporte preliminar. Rev Mex Ing Bioméd 1994;15:128-129.

  11. Borg E, Viberg A. Role of heating in non-invasive blood pressure measurement in rats. Acta Physiol Scand 1980;108:73-75.

  12. Infante-Vázquez O, Sánchez-Torres G, Martínez-Memíje R, Flores-Chávez P, Pastelin-Hernández G, Sánchez-Miranda M. Medición de la presión arterial utilizando el retardo en el pulso distal. Rev Bras Eng Bioméd 1997;13:81-92.

  13. Chiueh C, Kopin IJ. Hyperresponsivity of spontaneously hypertensive rat to indirect measurement of blood pressure. Am J Physiol 1978;234:690-695.




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