Negrete RP, Romero JJ, Villegas LG, Vázquez SVC

Language: English/Spanish
References: 17
Page: 289-295
PDF size: 67.21 Kb.

ABSTRACT

Following the analysis of the sanitary condition of production in fish farms from the state of Morelos in Mexico, which have used antibiotics continuously in order to prevent bacterial infection since all the fish farms have presented outbreaks, six different species of the Pseudomonadaceae family: Pseudomonas cepaciae, Pseudomonas diminuta, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas sutzerii and Pseudomonas vesicularis were isolated, purified and identified. These bacteria are pathogenic to both humans and fish. The alkaline lysis technique was used to obtain DNA from these bacteria. Antibiotic resistant plasmids of very high molecular weight were obtained from all of them. Base pair number was determined for all plasmids. We demonstrated the use of antibiotics as preventative medicine against bacterial infections in all of the farms sampled. The objective of this study was to identify the presence of plasmids that are resistant to antibiotics in bacteria from the Pseudomonadaceae family from ornamental fish from the Ciclidae, Poeciliae and Cyprinidae families, cultivated in fish farm from the state of Morelos, Mexico.

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