Gutiérrez-Pabello JA, Garry AL

Language: English/Spanish
References: 8
Page: 277-281
PDF size: 49.32 Kb.

ABSTRACT

Peripheral blood monocyte-derived macrophages from genetically resistant and susceptible cattle to intracellular parasites were evaluated for their ability to control the intracellular growth of virulent M. bovis. Bactericidal assays using a 10:1 multiplicity of infection were performed to study bacterial phagocytosis and bacterial multiplication at 0 and 24 h post-inoculation. The rate of bacterial phagocytosis was 1.4 ± 0.17 and 2.1 ± 0.54 bacteria/macrophage, whereas bacterial multiplication was 165% and 263.7% in macrophages from resistant and susceptible cattle respectively. These results confirm that macrophages from resistant and susceptible cattle differ significantly (P‹0.01) in controlling intracellular multiplication of virulent M. bovis.

REFERENCES

1. Hutt FB. Genetic resistance to disease in domestic animals. New York: Comstock Publishing Associates, 1958.

2. Skamene E. Genetic regulation of host resistance to bacterial infection. Rev. Infect Dis 1983;4:S823-832.

3. Haller O, Acklin M, Staeheli P. Genetic resistance to influenza virus in wild mice. Curr Top Microbiol Immunol 1986;127:331-337.

4. Harmon BG, Adams LG, Templeton JW, Smith R 3rd. Macrophage function in mammary glands of Brucella abortus-infected cows and cows that resisted infection after inoculation of Brucella abortus. Am J Vet Res 1989;50:459-465.

5. Price RE, Templeton JW, Smith R III, Adams LG. Ability of mononuclear phagocytes from cattle naturally resistant or susceptible to brucellosis to control in vitro intracellular survival of Brucella abortus. Infect Immun 1990;58:879-886.

6. Campbell GA, Adams LG, Sowa BA. Mechanisms of binding of Brucella abortus to mononuclear phagocytes from cows naturally resistant or susceptible to brucellosis. Vet Immunol Immunopathol 1994;41:295-306.

7. Qureshi T, Templeton JW, Adams LG. Intracellular survival of Brucella abortus, Mycobacterium bovis BCG, Salmonella dublin and Salmonella typhimurium in macrophages from cattle genetically resistant to Brucella abortus. Vet Immunol Immunopathol 1995;50:55-66.

8. Campbell GA, Adams LG. The long-term culture of bovine monocyte-derived macrophages and their use in the study of intracellular proliferation of Brucella abortus. Vet Immunol Immunopathol 1992;34:291-305.