medigraphic.com
ISSN 1561-3054 (Electronic)

2014, Number 3

<< Back Next >>

Rev Cubana Med Trop 2014; 66 (3)

In vitro efficacy of three endectocides against Triatoma infestans

Dadé MM, Daniele MR, Pía SM, Mestorino N

Language: Spanish
References: 30
Page: 338-350
PDF size: 315.35 Kb.


ABSTRACT

Introduction: various studies have demonstrated the role that areas around the houses play in domiciliary re-infestation by Triatoma infestans (kissing bugs). With the aim of removing residual foci of T. infestans that inhabit in neighboring areas of houses, different strategies have been developed. The administration of different anti-T. infestans compounds to animals living in areas around the houses might be a good way to reduce the risk of domiciliary re-infestation.
Objective: to evaluate the in vitro efficacy of three antiparasitic agents, doramectin (DRM), ivermectin (IVM) and eprinomectin (EPR) against fifth instar nymphs of Triatomainfestans.
Methods: artificial feeders were designed , which contained heparinized and fortified blood with various concentrations of the three endectocides (100-0.4 ng/mL). We used 600 fifth instar nymphs of T. infestans during the experiment. A group of insects were fed with untreated blood (control). After feeding they were under observation to check their condition every 24 hours for a week.
Results: the three molecules showed activity against T. infestans. In comparing the activity of the three molecules, DRM exhibited greater potency against insects, it even kept its activity against T. infestans at 0.4 ng/mL (lowest concentration tested). In the case of EPR and IVM, their efficacy began to lower at concentrations below 6.25 and 3.15 ng/mL respectively, being totally inactive at 0.4 ng/mL concentration.
Conclusions: Based on these results, we can assert that under our experimental conditions, IVM, EPR and DRM show in vitro high efficacy against T. infestans, being the latter more effective than the other two molecules.


REFERENCES

  1. Organización Panamericana de la salud. Estimación cuantitativa de la enfermedad de Chagas en las Américas. 2006;OPS/HDM/CD/425-06.

  2. Freiji H, Altech J. Congenital Chaga's disease: diagnostic and clinical aspects. Clin Infect Dis. 1995;21(3):551-5.

  3. Cancado JR. Criteria of Chagas disease cure. Mem. Inst. Oswaldo Cruz. 1999;94(1):331-6.

  4. Jörg ME. Límite sur de la dispersión geográfica de Triatoma infestans y su infestación por Trypanosoma cruzi en Argentina. Bol Of Sanit Panam. 1957;42(1):59-66.

  5. Oliveira Filho AM. Uso de nuevas herramientas para el control de triatominos en diferentes situaciones entomológicas en el continente americano. Rev.Soc. Bras. Med. Trop. 1997;30(1):41-6.

  6. Oliveira Filho AM. Differences of susceptibility of five triatomine species to pyrethroid insecticides-implications for Chagas disease vector control. Mem. Inst. Oswaldo Cruz. 1999;94(1):425-8.

  7. Picollo MI, Vassena CV, Santo Orihuela P, Barrios S. Hight resistance to pyrethroid insecticides aasociated with ineffective field treatments in Triatoma infestans (Hemiptera, Reduvidae) from the north of Argentina. J Med. Entomol. 2005;42(4):637-42

  8. Diotaiutti L, Vaz de Melo Azeredo B, UberBusek SC, Fernandes AJ. Controle do Triatoma sordida no peridomicilio rural do municipio de Porteirinha, Minas Gerais, Brasil. Rev Panam Salud Pública. 1998;3:21-5.

  9. Gürtler RE, Wisnivesky-ColliC, Solarz ND, Lauricella M, Bujas M. Dinámica de la transmisión de T. cruzi en una zona rural de la Argentina: II. Relación entre la infección doméstica en niños y perros y la densidad de Triatoma infestans infectados. Bol SanitPanam. 1988;104:130-43.

  10. Burg RW, Miller BM, Baker EE, Birnbaum J, Currie SA, Hartman R, et al. Avermectins, new family of potent anthelminthic agents producing organisam and fermentation. Antimicrob. Agents Chemother.1979;15(3):361–7.

  11. Forbes AB. A review of regional and temporal use of avermectins in cattle and horses worldwide. Vet. Parasitol. 1993;48:19–28.

  12. Borges FA, Silva HC, Buzzulini C, Soares VE, Santos E, Oliveira GP, et al.Endectocide activity of a new long-action formulation containing 2.25 % ivermectin+1.25% abamectin in cattle. Vet Parasitol. 2008;155(3-4):299-307.

  13. Turner MJ, Schaeffer JM. Mode of action of ivermectin. In: Campbell WC, editor. Ivermectin and Abamectin, New York: Springer Verlag. 1989;73-88.

  14. Fink DW, Porra AG. Pharmacokinetics of ivermectin in animals and humans. In: Campbell WC, editor. Ivermectin and Abamectin, New York: Springer Verlag.1989;113-30.

  15. Arieta-Román RJ, Rodríguez-Vivas RI, Rosado-Aguilar JA, Ramírez-Cruz GT, Basto-Estrella G. Moxidectina (10 %) e Ivermectina (3,15 %): evaluación de su eficacia y persistencia contra infestaciones naturales de Rhipicephalus (Boophilus) microplus en bovinos del trópico mexicano. Rev Mex Cienc Pecu. 2010;1(1):59-67.

  16. Aguirre DH, Gaido AB, Cafrune MM, Castelli ME, Mangold AJ, Guglielmone AA. Eprinomectin pour-on for control of Boophilus microplus (Canestrini) ticks (Acari: Ixodidae) on cattle. Vet Parasitol. 2005;127(2):157-63.

  17. Pereira JR. The efficiency of avermectins (abamectin, doramectin and ivermectin) in the control of Boophilus microplus, in artificially infested bovines kept in field conditions. Vet Parasitol. 2009;162(1-2):116-9.

  18. Shan Q, Haddrill JL, Lynch JW. Ivermectin, an unconventional agonist of the glycine receptor chloride channel. Journal of Biological Chemistry. 2001;276(16):12556-64.

  19. Núñez JA, Lazzari CR. Rearing of Triatomainfestans Klug (Het., Reduviidae) in the absence of a live host. Some factors affecting the artificial feeding. J Applied Entomol.1990;109:87-92.

  20. World Health Organization. Protocolo de evaluación de efecto insecticida sobre triatominos. WHO/UNDP/WB/TDR. Acta Toxic Argent. 1994;2(1-2):29-31.

  21. Oliveira Filho AM, Santos CE, Melo TMV, Figuereido MJ, Silva EL, Dias JCP, et al. Field trial of insecticides in an area of high levels of infestation and dispersion of Triatoma infestans. Mem Inst Oswaldo Cruz. 1986;81(Suppl):171.

  22. Dias JCP. Reinfestação do município de Bambuí por triatomíneos transmissores da doença de Chagas. Mem Inst Oswaldo Cruz. 1967;66:197-208.

  23. Cecere MC, Gürtler RE, Canale D, Chuit R, Cohen JE. El papel del peridomicilio en la eliminación de Triatoma infestans de comunidades rurales argentinas. BolOficSanitPanam. 1996;121:1-10.

  24. Wisnivesky-Colli C. La importancia del peridomicilio en un programa de eliminación de Triatoma infestans. Rev Soc Bras Med Trop. 1993;26(supl3):55-63.

  25. Carvajal G, Mougabure-Cueto G, Toloza AC. Toxicity of non-pyrethroid insecticides against Triatoma infestans (Hemiptera:Reduviidae).MemInst Oswaldo Cruz. 2012;107:675-9.

  26. Días JCP, Shofield CJ, Machado EMM, Fernandes AJ. Ticks, ivermectin, and experimental Chagas disease. Mem Inst Oswaldo Cruz. 2005;100(8):829-832.

  27. Martínez-Subiela S, Bernal LJ, Tvarijonaviciute A, Garcia-Martinez JD, Tecles F, Cerón JJ. Canine demodicosis: the relationship between response to treatment of generalised disease and markers for inflammation and oxidative status. Veterinary Dermatology. 2014;25(2):72-e24.

  28. Dadé M, Daniele M, Buchamer A, Marchetti ML, Mestorino N. Perfiles residuales de ivermectina en pollos parrilleros. 8vas Jornadas Internacionales de Veterinaria Práctica, organizadas por el Colegio de Veterinarios de la Provincia de Buenos Aires. 2013.

  29. Echeverria J, Mestorino ON, Errecalde JO. Comparative pharmacokinetics of ivermectin after its subcutaneous administration in healthy sheep and sheep infected with mange. Journal of Veterinary Pharmacology and Therapeutics. 2002;25:159-160.

  30. Mestorino N, Turic E, Pesoa J, Echeverría J, Errecalde O. Pharmacokinetics in plasma of ivermectin after its oral (solution and tablets) administration to sheep. Journal of Veterinary Pharmacology and Therapeutics. 2003;26(4):307-309.




2020     |     www.medigraphic.com