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2014, Number 3

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Rev Cubana Plant Med 2014; 19 (3)

Protective action of Duguetia furfuracea (A. St.-Hil.) Saff. against toxicity due to mercury chloride in Escherichia coli

Fernandes LCN, Francelino VT, Figueiredo LN, Batista ALB, Ferreira MEF, Regina KM, Melo CHD

Language: Portugués
References: 36
Page: 179-188
PDF size: 142.23 Kb.


ABSTRACT

Introduction: mercury is one of the most toxic metals. Contamination by mercury has been the object of many studies aimed at unraveling the mechanisms involved in its toxicity and its harmful effects on living beings. The species Duguetia furfuracea (A. St.-Hil.) Saff., popularly known asaraticum-bravo’, ‘ata-brava’ and ‘ata de lobo’, has been used in folk medicine as antirheumatic and for the treatment of renal dysfunction, spinal pain, stomach ache and pediculosis.
Objective: evaluate the cytoprotective effect of the ethanolic extract and fractions of D. furfuracea against the heavy metal mercury chloride (HgCl2 ).
Methods: characterization of secondary metabolites was performed by phytochemical screening, in which color change and precipitate formation are examined after the addition of specific reagents. Minimum inhibitory concentration (MIC) was determined by broth microdilution, and minimum bactericidal concentration (MBC) was verified using petri plates with HIA (heart infusion agar) to transfer the solutions incubated on microdilution plates.
Results: phytochemical screening revealed the presence of tannins, flavonoids an alkaloids. All samples exhibited a MIC ≥ 1024 µg/mL. MBC results showed that the ethanolic extract and all fractions of D. furfuracea except for the hexane fraction have cytoprotective activity by the bacterium E. coli against mercury chloride, an action attributed to the chelating properties of flavonoids.
Conclusions: data point to the promising value of the species D. furfuracea against heavy metals, presenting as protectors of procaryote beings. This is the first report on the use of medicinal plants as cytoprotective agents on the bacterial model.


REFERENCES

  1. Jarup L. Hazards of heavy metal contamination. British Medical Bulletins. 2003;68(1):167-182.

  2. Paula MT. Estudo das alterações comportamentais e bioquímicas induzidas pela exposição ao Hg (II) sobre o sistema antioxidante e vias de sinalização celular em Drosophila melanogaster. 2012. 94f. [Dissertação Mestrado]. Universidade Federal de Santa Maria, Santa Maria: Centro de Ciências Naturais e Exatas; 2012.

  3. Cogo AJD, Siqueira AF, Ramos AC, Cruz ZMA, Silva AG. Using oxidative stress enzymes as biomarkers in environmental impacts. Natureza online. 2009;7(1):37-42.

  4. Cursino L, Mattos SVM, Silva NO, Chartone-Souza E, Nascimento AMA. Measurement of volatilized mercury by a mini-system: a simple, reliable and reproducible technique. Brazilian Archives of Biology and Technology. 2003;46(4):731-734.

  5. Mendes PLA, Meyer ST, Noronha IAS, Gomes SMA, Santos MH. Alterações morfológicas em Eichhornia crassipes (Aguapè) (Mart.) Solms-Laubach (Pontederiaceae), exposta a elevadas concentrações de mercúrio. Pesticidas: Revista de Ecotoxicologia e Meio Ambiente. 2009;19(0):30-36.

  6. Lemos RMA, Pinto FN, Guimarães JRD, Bianchini Jr. L, Fostier AH, Forti MC, et al. Macrófitas aquáticas e sedimentos como indicadores de Hg a jusante do garimpo do Tartarugalzinho, AP, Brasil. [CDROM]. In: IV Simpósio de Ecossistemas Brasileiros, 1998, Águas de Lindóia. Anais. São Paulo: Academia de Ciências do Estado de São Paulo, 02 a 07 de abril; 1998.

  7. Esteves FA. Fundamentos de limnologia. 2. ed. Rio de Janeiro: Interciência, 1998. p. 602.

  8. Azevedo FA. Toxicologia do mercúrio. São Carlos/SP: Editora Ri Ma, 3002. p. 272.

  9. Boujbiha MA, Hamden K, Guermazi F, Bouslma A, Omezzine A, Kammoun A, et al. Testicular toxicity in mercuric chloride treated rats: association with oxidative stress. Reproductive Toxicology. 2009;28(1):81-89.

  10. Aguiar MRMP, Novaes AC, Guarino AWS. Remoção de metais pesados de efluentes industriais por aluminos silicatos. Química Nova. 2002;25(6):1145-1154.

  11. Cardoso PCS, Lima PDL, Bahia MO, Amorim MIM, Burbano RR, Farias RAF. Efeitos Biológicos do Mercúrio e seus Derivados em Seres Humanos - uma revisão bibliográfica. Revista Paraense de Medicina. 2001;15(4):51-58.

  12. Olaniran AO, Naicker K, Pillay B. Antibiotic resistance profiles of Escherichia coli isolates from river sources in Durban, South Africa. World Journal of Microbiology & Biotechnology. 2009;25(10):1743-1749.

  13. Cervantes C, Gutierrez-Corona F. Copper resistance mechanisms in bacteria and fungi. FEMS Microbiology Reviews. 1994;14(2):121-138.

  14. Wuertz S, Mergeay M. The impact of heavy metals on soil microbial communities and their activities. In: Van Elsas JD, Wellington EMH, Trevors JT, editores. Modern Soil Microbiology. Nova Iorque: Marcel Dekker; 1997. p. 1-20.

  15. Vetriani C, Chew YS, Miller SM, Yagi J, Coombs J, Lutz RA, et al. Mercury adaptation among bacteria from a deep-sea hydrothermal vent. Applied and Environmental Microbiology. 2005;71(1):220-226.

  16. Zeyaullah M, Nabi G, Malla R, Ali A. Molecular studies of E. coli mercuric reductase gene (merA) and its impact on human health. Nepal Medical College Journal. 2007;9(3):182-185.

  17. Brito IC. A importância dos bioindicadores vegetais no ambiente aéreo, aquático e terrestre – plantas indicadoras do mercúrio. [CDROM]. In: XXXIV Congresso Nacional de Botânica, 1983, Porto Alegre. Anais. Porto Alegre: Sociedade Botânica do Brasil/Universidade Federal do Rio Grande do Sul, 23 a 29 de janeiro; 1983.

  18. Floriani SC. Atividade antimicrobiana do extrato hexânico de Drimys angustifólia. 2010. 33f. [Monografia]. Universidade Regional de Blumenau, Blumenau: Centro de ciências da Saúde; 2010.

  19. Agra MF, França PF, Barbosa-Filho JM. Synopsis of the plants known as medicinal and poisonous in Northeast of Brazil. Revista Brasileira de Farmacognosia. 2007;17(1):114-140.

  20. Pott A, Pott VJ. Plantas do pantanal. Brasília, DF: EMBRAPA-SPI; 1994. p. 320.

  21. Gavilanes ML, Brandão M. Plantas consideradas medicinais ocorrentes na Reserva Biológica Municipal do Poço Bonito, Município de Lavras, Minas Gerais. Daphne 1998;8(2):57-68.

  22. Gottsberge IS. O cerrado como potencial de plantas medicinais e tóxicas. In: Simpósio de Plantas Medicinais do Brasil. Oréades. 1982;8(14-15):15-30.

  23. Lorenzi H, Matos FJA. Plantas medicinais no Brasil: nativas e exóticas. Nova Odessa: Instituto Plantarum; 2002. p. 512.

  24. Matos FJA. Introdução à fitoquímica experimental. 2. ed. Fortaleza: Editora UFC; 1997. 148 p.

  25. Coutinho HDM, Costa JGM, Lima EO, Falcão-Silva VS, Siqueira-Júnior JP. Enhancement of the Antibiotic Activity against a Multiresistant Escherichia coli by Mentha arvensis L. and Chlorpromazine. Chemotherapy. 2008;54(4):328-330.

  26. Válega M, Lillebo AI, Pereira ME, Caçador I, Duarte AC. Mercury in salt marshes ecosystems: Halimione portulacoides as biomonitor. Chemosphere. 2008;73(8):1224-1229.

  27. Santos FS, Sobrinho NMBA, Mazur N, Garbisu C, Barrutia O, Becerril JM. Resposta antioxidante, formação de fitoquelatinas e composição de pigmentos fotoprotetores em Brachiaria decumbens Stapf submetida à contaminação com Cd e Zn. Química Nova. 2011;34(1):16-20.

  28. Sousa A, Caçador I, Lillebo A, Pardal M. Heavy metal accumulation in Halimione portulacoides: Intra- and extra-cellular metal binding sites. Chemosphere. 2008;70(5):850-857.

  29. Hall JL. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany, Oxford. 2002;56(366):1-11.

  30. Hulin V, Mathot AG, Mafart P, Dufosse L. Les proprietés anti-microbiennes des huiles essentielles et composes d’arômes. Sciences des Aliments. 1998;18(6):563-582.

  31. Alves E. Atividade antioxidante de extratos de própolis comercializados em Santa Maria – RS e aplicação em linguiça toscana refrigerada. 2009. 68f. [Dissertação Mestrado]. Universidade Federal de Santa Maria, Santa Maria: Centro de Ciências Rurais; 2009.

  32. Melo AE, Guerra NB. Ação antioxidante de compostos fenólicos naturalmente presentes em alimentos. Boletim da Sociedade Brasileira de Ciência e Tecnologia de Alimentos. 2002;36(1):1-11.

  33. Harborne JB, Williams CA. Advances in flavonoid research since 1992. Phytochemistry. 2000;55(6):481-504.

  34. Behling EB, Sendão MC, Francescato HDC, Antunes LMG, Bianchi MLP. Flavonoid quercetin: general aspects and biological actions. Alimentary Nutrition. 2004;15(3):285-292. 33. Harborne JB, Williams CA. Advances in flavonoid research since 1992. Phytochemistry. 2000;55(6):481-504. Flavonoid quercetin: general aspects and biological actions. Alimentary Nutrition. 2004;15(3):285-292.

  35. Smith DS, Bell RA, Kramer JR. Metal speciation in natural waters with emphasis on reduced sulfur groups as strong metal binding sites. Comparative Biochemistry and Physiology. 2002;133(1-2):65-74.

  36. Stevenson FJ. Humus Chemistry: genesis, composition, reactions. 2. ed. New York: John Wiley; 1994. p. 496.




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