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2014, Number 3

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Rev Cubana Plant Med 2014; 19 (3)

Validation of a High Performance Liquid Chromatography technique for determination of mangiferin content in Mangifera indica L. leaves

Romero DJA, Nuva-Paz L, López M, Ferrada C, Carballo C

Language: Spanish
References: 39
Page: 167-178
PDF size: 172.72 Kb.


ABSTRACT

Introduction: Mangiferin is a natural glycosylxanthone present in various parts of the tree Mangifera indica L. which is attributed a wide range of pharmacological properties.
Objective: establish a method to determine the content of mangiferin in Mangifera indica L. leaves by high performance liquid chromatography.
Methods: leaves were dried and pulverized before quantification of their metabolite content. Separation was performed using a Luna RP-18 chromatographic column (5 µm) (250 x 4mm) with a UV detector at 254n and a mobile phase A gradient composed of acetic acid 0.2 %: acetonitrile (85:15), and a phase B mobile: acetonitrile and its quantification vs. a reference sample applying the external standard method.
Results: the regression curve equation for mangiferin was Y=3.5561X +6536.55(r=0.999). Recovery was 99.41 % with a variation coefficient below 2 %. Detection and quantification limits were 0.0009 and 0.001, respectively. No statistically significant differences were found between the determinations performed on different days by different analysts.
Conclusion: the method is simple and reliable, and may be used for quality control and stability studies about Mangifera indica L. leaves.


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