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2014, Number 3

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Rev Cubana Plant Med 2014; 19 (3)

Evaluation of the biological activity of extracts from Crotalaria pallida (streaked rattlepod) seeds using the Drosophila melanogaster model

Peñaloza AGC, Peláez JCA

Language: Spanish
References: 17
Page: 144-153
PDF size: 182.11 Kb.


ABSTRACT

Introduction: the search for metabolites of natural origin with promising biological –particularly insecticidal– activity, is an interesting target for research about natural products.
Objectives: evaluate the bioactivity of extracts of varying polarity from Crotalaria pallida Aiton seeds using the Drosophila melanogaster biological model.
Methods: an evaluation was conducted of the bioactivity of extracts of varying polarity from dry C. pallida seeds by ingestion into the biological model, with the purpose of purifying and determining the chemical structure of the active principle through NMR spectroscopy.
Results: two types of bioactivity were expressed. One caused inhibition of larval stages, evidenced in a smaller number of pupae in treatment controls with respect to non-treatment controls. The dose-response relationship allowed estimation of a CI50 of 156.47 ppm. The other effect inhibited progress from pupa to adult, reducing the number of adults in the treatment vs. control groups, with an CI50 of 7.95 ppm. With the use of various NMR assays, it was determined that the alkaloid usaramine was responsible for this biological activity.
Conclusions: the bioactivity of extracts of medium/low polarity permitted the isolation of a metabolite with promising insecticidal activity, manifested in the inhibition of the normal development of the life cycle of D. melanogaster. The extract does not show any activity on oviposition in the concentration range studied. At low concentrations it inhibits the eclosion of pupae, whereas at high concentrations it affects larval development. Both activities remained when the purified metabolite was tested.


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