Oliva-Hernández R, García-Sánchez HM, Infante-Bourzac JF, Pérez-Quiñoy JL, Cedré-Marrero B, Valmaseda-Pérez T, Talavera-Coronel A, Año-López G, Pérez-Amat VM, García-Imía L

Language: Spanish
References: 23
Page: 100-109
PDF size: 312.91 Kb.

ABSTRACT

In many countries, Cholera remains being a problem for human health, since it continues being an epidemic or endemic disease, which affects either children or adults and it is a death cause when cholera cases are not treated. A live oral vaccine against this disease can contribute to control important epidemics, mainly at countries where the sanitary hygienic conditions are not good or drinking water is not available. In this work, bio models were selected and applied for the selection of genetically attenuated Vibrio cholerae strains as vaccine candidates against cholera. Their toxigenicity, immunogenicity and protection were evaluated by means of Ileal Loops, intraduodenal inoculation and RITARD models in F1 (New Zealand x White Semi giant) young adults rabbits weighing from 1.8 to 2.3 kg. The results obtained with the used bio models, show that the Vibrio cholerae genetically attenuated strains are no toxigenic, but are immunogenic and protective. As conclusion, these bio models are available for the evaluation and selection of oral vaccine candidates.

REFERENCES

1. Liang W, Want S, Yu F, Zhang L, Qi G, Liu Y, et al. Construction and evaluation of a safe, live oral Vibrio cholerae vaccine candidate. IEM108. Infect Immun 2003;71(10):5498-504.

2. Ledón T, Ferrán B, Pérez C, Suzarte E, Vichi J, Marrero K, et al. TLP01, an msha mutant of Vibrio cholerae O139 as vaccine candidate. Microbes and Infection 2012;14:968-78.

3. García L, Oliva R, Cedré B, Valmaseda T, García H, Talavera A, et al. Intraduodenal inoculation of adults rabbits for evaluating the immunogenicity of genetically attenuated Vibrio cholerae strains. Lab Animal Science 1998;48(5):267- 70.

4. Eko FO, Schukovskaya T, Lotzmanova EY, Firstova VV, Emalyanova NV, Klueva SN, et al. Evaluation of the protective efficacy of Vibrio cholerae ghost (VCG) candidate vaccines in rabbits. Vaccine 2003;21(25-26):3663-74.

5. Russell RG, Tall BD, Morris JG. Non-O1Vibrio cholerae intestinal pathology and invasion in the removable intestinal tie adult rabbit diarrhea model. Infect Immun 1992;60(2):435- 42.

6. De SN, Chatterjee DN. An experimental study of the mechanism of action of Vibrio cholerae on the intestinal mucous membrane. J Pathol Bacteriol 1953;66:559-63.

7. Pérez JL, García L, Talavera A, Oliva R, Valmaseda T, Año G, et al. Passive protection of serum from volunteers inoculated with attenuated strain 638 of Vibrio cholerae O1 in animal models. Vaccine 2001;19:376-84.

8. Spira WW, Sack RB. Kinetics of early cholera infection in the removable intestinal tie-adult rabbit diarrhea model. Infect Immun 1982;35:952-7.

9. Kirn TJ, Taylor RK. TcpF is a soluble colonization factor and protective antigen secreted by El Tor and classical O1 and O139 Vibrio cholerae serogroups. Infect Immun 2005;73(8):4461-70.

10. Sack RB, Carpenter CJ. Experimental canine cholera. I. Development of the model. J Infect Dis 1969;119:138-49.

11. Sack RB, Johnson J, Pierce NF, Keren DF, Yardley JH. Challenge of dogs with live enterotoxigenic Escherichia coli and effects of repeated challenges on fluid secretion in jejunal Thiry-Vella loops. J Infect Dis 1976;134(1):15-24.

12. Isupov IV, Podoprigora GI, Dushkin VA, Gorkova AV, Nazarova LS. Use of gnotobiotic animals for the study of the pathogenesis of cholera intoxication. Zh Mikrobiol Epidemiol Immunobiol 1988;12(12):44-8.

13. López Y, Infante JF, Sifontes S, Díaz D, Pérez V, Año G, et al. Pharmacology and toxicology of an oral tablet whole cells inactivated cholera vaccine in Sprague Dawley rats. Vaccine 2011;29(19):3596-9.

14. Lobanov VV, Kolpikova LD, Sharikov AM, Gavrilov BB. Experimental study of mixed infection by Vibrio cholerae and Salmonella typhi. Zh Mikrobiol Epidemiol Immunobiol 1998;5(9):17-9.

15. Infante JF, Sifontes S, Sierra G, Campa C, Fariñas M, Oliva R, et al. Los biomodelos aplicados al desarrollo de vacunas y sueros en el Instituto Finlay. Animales de Experimentación, La Revista Hispanoamericana 1998;3(3):30-40.

16. Oliva R, García H, Infante JF, García L, Pérez JL, Cedré B, et al. Biomodelo para la evaluación de cepas atenuadas como candidatos vacunales contra el cólera humano. I. Estudio de la virulencia, capacidad de colonización y adherencia a la mucosa intestinal. VacciMonitor 2008;17(1):1-6.

17. Cedré B, García HM, García LG, Talavera A. Estandarización y evaluación del ensayo vibriocida modificado. Revista Cubana de Medicina Tropical 1999;51(3):156-9.

18. Alvarez N, Sarmiento ME, Mohd-Nor N, Acosta A. Uses of immunoglobulin A in the control of the infectious. Biotecnología Aplicada 2014;31(1):1-6.

19. Vacca LL. Laboratory Manual of Histochemestry. New York: De Raven Press Books; 1985.

20. Chatterjee SN, Chaudhuri K. Lipopolysaccharides of Vibrio cholerae. I. Physical and chemical characterization. Biochim Biophys Acta 2003;1639(2):65-79.

21. Ledón T, Ferrán B, Vichi J, Suzarte E, Oliva R, Fando R. Evaluación en modelos animales de cepas vivas atenuadas de Vibrio cholerae O139. Revista CENIC Ciencias Biológicas 2010;41(Edición Especial 1):1-12.

22. Kang SS, Yang JS, Kim KW, Yun CH, Holmgren J, Czerkinsky C, et al. Anti-bacterial and anti-toxic immunity induced by a killed whole-cell-cholera toxin B subunit cholera vaccine is essential for protection against lethal bacterial infection in mouse pulmonary cholera model. Mucosal Immunology 2013;6(4):826-37.

23. Kondo M, Yamato M, Takagi R, Murakami D, Namiki H, Okano T. Significantly different proliferative potential of oral mucosal epithelial cells between six animal species. J Biomed Mater Res 2014;102(6):1829-37.