medigraphic.com
ISSN 1025-0298 (Electronic)
ISSN 1025-028X (Print)

2014, Number 3

<< Back Next >>

VacciMonitor 2014; 23 (3)

Protection study of the Labio-Marek vaccine against the JM strain challenge in SPF chickens

Paneque-Sayas M, Contreras-González D, Fresneda A, Proenza G, Tabares-Beltrán TI, Diaz-Fernández E

Language: Spanish
References: 19
Page: 91-99
PDF size: 325.82 Kb.


ABSTRACT

Marek's disease is a lymphomatous and neuropathic disease of the domestic chickens caused by herpesvirus. Currently the acute form of the disease, with extended visceral lymphomas, is the most common with an incidence of 10-30%. The objective of the present study was to evaluate the effectiveness of the vaccine Labio-Marek prepared by LABIOFAM against the challenge of the virulent JM strain in specific pathogen free chickens. A dose of the vaccine containing 1000 plate-forming units in 0.2 mL was inoculated in one-day old chickens by subcutaneous route. The chickens were challenged with 103 TCID50 of the JM strain and observed during 7 weeks to detect neurological signs such as paralysis and others associated with the disease. One group of chickens remained as a control of non-vaccinated chickens, which were challenged, and another group of chickens that was not vaccinated nor challenged. Autopsy was carried out at day 54 after vaccination on chickens that survived the challenge and histological evaluation of the organs was performed. The vaccinated chickens showed no clinical symptoms of the disease and had no microscopic lesions related to Marek's disease virus. However, 71% of non-vaccinated challenged chickens died and 83.87% showed lesions of Marek's disease. It is concluded that the evaluated vaccine can be used for the prevention of Marek's disease in chickens.


REFERENCES

  1. Marek J. Multiple Nervenentzündung (Polyneuritis) bei Hühnern. Deut Tierarztl Woch 1907;15:417-21.

  2. Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA. Virus taxonomy; Eighth Report of the International Committee on the Taxonomy of Viruses. San Diego: Elsevier Academic Press;2005.

  3. Biggs PM, Nair V. The long view: 40 years of Marek's disease research and Avian Pathology. Avian Pathol 2012;41(1):3-9.

  4. Carvallo FR, French RA, Gilbert-Marcheterre K, Risatti G, Dunn JR, Forster F, et al. Mortality of one-week-old chickens during naturally occurring Marek's disease virus infection. Vet Pathol 2011;48:993-8.

  5. Gimeno IM, Witter RL, Cortes AL, Reddy SM, Pandiri AR. Standardization of a model to study revaccination against Marek's disease under laboratory conditions. Avian Pathol 2012;41(1):59-68.

  6. Code of Federal Regulations USDA, FDA. Immunogenicity. Title 9, vol 1 sec. 113.330. California: FDA;2014.

  7. AVMA. American Veterinary Medical Association Guidelines on Euthanasia. Schaumburg: AVMA;2013.

  8. Schat KA, Markowski-Grimsrud CJ. Immune responses to Marek’s disease virus infection. Curr Topics in Microbiol Immunol 2001;255:91-120.

  9. Gimeno IM, Cortes AL, Witter RL, Pandiri AR. Optimization of the protocols for double vaccination against Marek's disease using commercially available vaccines: evaluation of protection, vaccine replication, and activation of T cells. Avian Dis 2012;56:295-305.

  10. Haq K, Faizal MAC, Shanmuganthan S, Thanthrige-Don N, Read LR, Sharif S. Vaccine-induced host responses against very virulent Marek's disease virus infection in the lungs of chickens. Vaccine 2010;28(34):5565-72.

  11. Haq K, Fear T, Ibraheem A, Abdul-Careem MF, Sharif S. Influence of vaccination with CVI988/Rispens on load and replication of a very virulent Marek's disease virus strain in feathers of chickens. Avian Pathol 2012;41(1):69-75.

  12. Kingham BF, Zelnik V, Kopacek J, Majerciak V, Ney E, Schmidt CJ. The genome of herpesvirus of turkeys: comparative analysis with Marek's disease viruses. J Gen Virol 2001;82:1123-35.

  13. Bublot M, Sharma J. Vaccination against Marek's disease. In: Davison TF, Nair V. Marek's Disease–An Evolving Problem. London: Academic Press; 2004.p.168-85.

  14. Gimeno IM. Marek’s disease vaccines: A solution for today but a worry for tomorrow? Vaccine 2008;26(Supplement 3):31-41.

  15. Sevoian M, Chamberlain DM, Counter FT. Avian lymphomatosis. I. Experimental reproduction of the neural and visceral forms. Veterinary Medicine 1962;57:500-1.

  16. Dunn JR, Silva RF, Lee LF, Witter RL. Competition between two virulent Marek's disease virus strains in vivo. Avian Pathol 2012;41(3):267-75.

  17. OIE. Marek´s disease. 2.3.13 Chapter. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Paris: OIE; 2012. Disponible en: http://www.oie.int/fileadmin /Home/eng/Health_standards/tahm/2.03.13_MAREK_DIS.pdf.

  18. Gimeno IM, Witter RL, Reed WM. Four distinct neurologic syndromes in Marek's disease: effect of viral strain and pathotype. Avian Dis 1999;43:721-37.

  19. Morales OE. La enfermedad de Marek como factor inmunodepresor. En: AMEVEA. Memorias I seminario técnico avícola. Santa Cruz: AMEVEA–Bolivia;1993.p.1-3.




2020     |     www.medigraphic.com