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ISSN 1561-3054 (Electronic)

2014, Number 2

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Rev Cubana Med Trop 2014; 66 (2)

Expression of IFN g and TNF α genes in tissues from dengue fatal cases

Pérez DAB, Pérez GOL, Sierra VBC, Aguirre PE, García MG, Álvarez VM, Gonzalez RD, Limonta VD, Rosario DD, Izquierdo OA, Guzmán TMG

Language: Spanish
References: 22
Page: 286-294
PDF size: 120.48 Kb.


ABSTRACT

Introduction: dengue is considered to be the most important arthropod-borne human disease worldwide, with an estimated 100 million new cases per year and more than 20 000 deaths. The pathogenesis of severe dengue is not fully understood. However, certain studies have associated it with sequential infections by different viral serotypes, memory T cell activation and cytokine hyperproduction.
Objectives: determine the qualitative expression of genes of cytokines IFNγ and TNFα in spleen and liver tissues from dengue 3 and 4 fatal cases, detecting RNAm and verifying their role in the pathogenesis of the disease.
Methods: the study was conducted by retrotranscription and polymerase chain reaction (RT-PCR) using specific primers.
Results: expression of IFNγ predominated over that of TNFα, and was more evident in spleen than in liver tissues.
Conclusions: results support the pathogenic role of the Th1 response pattern in severe dengue. This is the first study of its type about dengue infection.


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