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2014, Number 2

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RCAN 2014; 24 (2)

Serogroups and antimicrobial resistance of Eschericha coli strains isolated in foods from outbreaks of diarrhea

Puig PY, Leyva CV, Apórtela LN, Campos GN, Frerer MY, Soto RP

Language: Spanish
References: 23
Page: 161-167
PDF size: 234.02 Kb.


ABSTRACT

Serological groups and antimicrobial resistance of 74 strains of Escherichia coli isolated from foods involved in food-borne diseases episodes were determined at the Laboratory of Food Microbiology, Nutrition and Food Hygiene Institute, Havana City (Cuba). Serotyping was performed by means of the slide agglutination method. Antimicrobial susceptibility was determined by the Kirby-Bauer disk diffusion method. Forty-two (56.7%) of the isolates were typed. The most common serological groups were (in descending order) O114, O26, O55, O126, and O128. Antibiograms were completed in 55 (74.3%) of the strains. Sixty-one point eight percent of assayed strains exhibited resistance to one (or more) of the 11 antibiotics tested. Four antimicrobial multi-resistance patterns to 5 (or more) antibiotics were found. Resistance to ampicillin was prevalent, presenting in 36.4% of the assayed isolates. The number of cephalosporin- and quinolones-resistant E. coli strains increased with respect from previous studies. Although antimicrobial resistance of the studied E. coli strains does not reach the magnitude reported in other countries, nonetheless this study has shown Cuba is not an exemption to this problem. Discussed results should constitute a signal of alarm leading to the design and implementation of adequate policies for the use of antimicrobials.


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