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2014, Number 4

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Rev Hematol Mex 2014; 15 (4)

Micrometric determination of the most frequent leukemic blasts’ size

Jaime-Pérez JC, Esparza-Sandoval AC, Vázquez-Mellado-De Larracoechea A, López-Serna N, Álvarez-Cantú R, Vázquez-Garza E, Gómez-Almaguer D

Language: Spanish
References: 15
Page: 157-163
PDF size: 445.90 Kb.


ABSTRACT

Background: The morphology of malignant cells that proliferate in the different leukemias, including their size, is very heterogeneous; there is scarce specific information regarding the size of these cells, which can be of relevance to explain some clinical characteristics, like development of hyperviscosity syndrome and thrombotic events.
Objective: To determine the size of leukemic blasts in the most frequent acute leukemias.
Material and method: An ambispective, observational study was done in which were stained with Wright’s stain marrow smears of patients treated at the Hematology Service of the University Hospital Dr. Jose Eleuterio Gonzalez, Autonomous University of Nuevo Leon, Mexico, diagnosed with acute lymphoblastic leukemia, acute myeloblastic leukemia and chronic myelogenous leukemia in blastic phase. Lineage was established by flow cytometry. Clinical stages included diagnosis, induction therapy and relapse. Those with the most representative morphologic characteristics of each acute leukemia subtype were selected for micrometry measurement. Data were analyzed with descriptive statistics, obtaining median and range; size was compared with those found in the literature.
Results: Leukemic blasts of 186 patients were studied; 111 with acute lymphoblastic leukemia, 55 with acute myeloblastic leukemia and 20 with chronic myelogenous leukemia in blastic phase. The medians and ranges of the cells size were: 11.3 µ (4.5-17.2), 11.2 µ (8.3-15.7) and 11.7 µ (6.6-15.0), respectively.
Conclusion: Despite the different cell origin and clinical stage of the acute leukemias, the median size and its range was remarkably similar among the studied patients.


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