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ISSN 1561-3011 (Electronic)

2014, Number 1

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Rev Cubana Invest Bioméd 2014; 33 (1)

Validation of an immunochemical technique for the detection of fecal occult blood

Hano GOM, Castellanos GM, Calzadilla BL, Villa JOM

Language: Spanish
References: 42
Page: 19-33
PDF size: 437.77 Kb.


ABSTRACT

Background: colorectal cancer is preventable and responds effectively to treatment when diagnosed at an early stage. Determination of fecal occult blood is a method used to screen for early colorectal cancer and its precursor lesion, the adenoma.
Objectives: 1) determine the validity and reproducibility of the RapiLat-Hemo immunochemical test to diagnose colorectal cancer and adenomas, and compare it with the reference reagent (SPIN-FOB), 2) describe the most frequent endoscopic findings in patients with positive fecal occult blood, 3) describe the location of colorectal cancer in patients with positive RapiLat-Hemo immunochemical tests.
Methods: A validation study was conducted of a new Cuban immunochemical test (RapiLat-Hemo) to detect FOB. The study sample was composed of 161 patients attending colon consultation at the Institute of Gastroenterology of Havana in the period 2008-2010 who were undergoing colonoscopy and met the inclusion criteria. All patients underwent FOB determination (RapiLat-Hemo), and results were correlated with those of the reference reagent and colonoscopy.
Results: There was a predominance of the female sex and the ≤ 60 age group. The test for detection of colorectal cancer had a sensitivity of 88.2% and a specificity of 92.4%. For detection of ≥ 10 mm adenomas, sensitivity was 60.0% and specificity 85.3%.
Conclusions: The test showed adequate validity and reproducibility for the diagnosis of colorectal cancer and ≥ 10 mm adenomas. There was concordance with the reference reagent (SPIN-FOB). The most common endoscopic findings were polyps, colorectal cancer and diverticula. The most common colorectal cancer location in patients with positive fecal occult blood was the most distal segments of the colon.


REFERENCES

  1. Rex DK, Liangpunsakul S. Detección del cáncer colorrectal. The American College of Gastroenterology. (Consultado junio 2008). Disponible en: http://patients.gi.org/recursos-en-espanol/deteccion-del-cancer-colorectal/

  2. Guía de Recomendaciones para la prevención del cáncer colorrectal. Consenso argentino 2004. (Consultado junio 2008). Disponible en: http://www.sacp.org.ar/consenso_argentino_2004.pdf

  3. Viñes JJ, Ardanaz E, Aráosla A, Gaminde L. Epidemiología poblacional de cáncer colorrectal: revisión de la causalidad. (Consultado abril 2008). Disponible en: http://www.cfnavarra.es/salud/anales/textos/vol26/n1/salud1a.html

  4. Béjar L, Pili M, Díaz V, Ramírez G, López J, Cabanillas JL. Incidence and mortality by colorectal cancer in Spain during 1951-2006 and its relationship with behavioural factors. European Journal of Cancer Prevention. 2009;18:436-44.

  5. Cuba. Dirección Nacional de Estadísticas. Anuario estadístico de 2007: Principales causas de muerte de todas las edades. [en línea]. La Habana: MINSAP; 2008. (Consultado mayo 2010). Disponible en: http://bvs.sld.cu/cgibin/wxis/anuario/?IsisScript=anuario/iah.xis&base=anuario&lan g=e

  6. Torres Vidal RM, Gran Álvarez MA. Impacto del cáncer de colon en la morbilidad de la población cubana,1979-2003. Rev Temas estadísticos de Salud. Dirección Nacional de Estadísticas. MINSAP. 2005;1(1). (Consultado mayo 2008). Disponible en: http://www.sld.cu/galerias/pdf/sitios/dne/ nro1_cancer_colon.pdf

  7. MINSAP. Dirección Nacional de Estadísticas. Anuario estadístico de 2007: Mortalidad por algunos tumores malignos según sexo. [en línea]. La Habana: Cuba, 2008 (Consultado marzo 2009). Disponible en: http://bvs.sld.cu/cgibin/wxis/anuario/?IsisScript=anuario/iah.xis&base=anuario&lang =e

  8. Marzo-Castillejo M, Bellas-Beceiro B, Nuin-Villanueva M, Cierco-Peguera P, Moreno- Baquerano M, Rubio-Toledano L. Grupo de prevención del cáncer del PAPPS. Actualizaciones 2005. Aten Prim. 2005;36(Supl 2):45-65.

  9. Study in The European Journal of cancer Prevention National Bowel Cancer Screening Program. J Med Screen. 2008;15:163-74.

  10. Rex DK, Johnson DA, Lieberman DA, Burt RW, Sonnenberg A. Colorectal cancer prevention 2000: screening recommendations of the American College of Gastroenterology. Am J Gastroenterol. 2000;95(4):868-77.

  11. Smith RA, Von Eschenbach AC, Wender R, Levin B, Byers T, Rothenberger D. ACS Prostate Cancer Advisory Committee, ACS Colorectal Cancer Advisory Committee, ACS Endometrial Cancer Advisory Committee. American Cancer Society guidelines for the early detection of cancer: update of early detection guidelines for prostate, colorectal, and endometrial cancers. Also: Update 2001-Testing for early lung cancer detection. CA Cancer J Clin. 2001;51:38-75.

  12. Asociación Española de Gastroenterología, Sociedad Española de Medicina de Familia y Comunitaria y Centro Cochrane Iberoamericano. Prevención del cáncer colorrectal. Guía de Práctica Clínica. Barcelona, marzo de 2004. (Consultado mayo 2010). Disponible en: http://www.sepd.org

  13. Sáenz R, Navarro A. Una proposición de protocolos de diagnóstico y tratamiento para países de Latinoamérica. Cáncer Colorrectal. Reunión de Consenso. 20 de octubre de 2004. Santiago de Chile. (Consultado mayo 2010). Disponible en: http://www.aige.org

  14. Ouyang DL, Chen JJ, Getzenberg RH, Schoen RE. Noninvasive testing for colorectal cancer: A review. Am J Gastroenterol. 2005;100(6):1393-403.

  15. Woo HY, Mok RS, Park YN, Park DI, Sung IK, Sohn ChI. A prospective study of a new immunochemical fecal occult blood test in Korean patients referred for colonoscopy. Clinical Biochemistry. 2005;38:395-9.

  16. Crespil M, Lisi D. Is colorrectal cancer screening by fecal occult blood feasible? Annals of Oncology. 2002;13:47-50.

  17. Church TR, Yeazel MW, Jones RM. A randomized trial of direct mailing of fecal occult blood tests to increase colorectal cancer screening. J Natl Cancer Inst. 2004;96:770-80.

  18. Ko CW, Dominitz JA, Nguyen TD. Fecal occult blood testing in a general medicine clinic: comparison between guaiac-based and immunochemical based tests. Am J Med. 2003;115:111-4.

  19. Imperiale TF. Quantitative immunochemical fecal occult blood tests: is it time to go back to the future? Ann Intern Med. 2007;146:309-11.

  20. Morikawa T, Kato J, Yamaji Y, Wada R, Mitsushima T, Shiratori Y. A comparison of the immunochemical fecal occult blood test and total colonoscopy in the asymptomatic population. Gastroenterology. 2005;129:422-8.

  21. Bampton PA, Sandford JJ, Cole SR, Smith A, Morcom J, Cadd B, et al. Interval faecal occult blood testing in a colonoscopy based screening programme detects additional pathology. Gut. 2005;54:803-6.

  22. Levi Z, Hazazi R, Rozen P, Vilkin A, Waked A, Niv Y. A quantitative immunochemical faecal occult blood test is more efficient for detecting significant colorectal neoplasia than a sensitive guaiac test. Aliment Pharmacol Ther. 2006;23:1359-64.

  23. Declaración de Helsinki de la Asamblea Médica Mundial. Principios éticos para las investigaciones médicas en seres humanos. 52a Asamblea General [monografía en Internet]. Escocia: Asamblea Médica Mundial; 2000. (Consultado marzo 2008). Disponible en: http://www.upo.es/general/investigar/otri/otri_docu/pn/Decl_Helsinki.pdf

  24. Levi Z, Rozen P, Hazazi R. Sensitivity and specificity of a quantitative immunochemical FOBT for colorectal neoplasia. Ann Intern Med. 2007;146:244-55.

  25. McLoughlin RM, Colm AO. Colorectal cancer screening. World J Gastroenterol. 2006;12(42):6747-50.

  26. Tusen Y, Chao L, Barroso L, Armas M, Fernández I. Test de sangre oculta y colonoscopia en el diagnóstico del cáncer colorrectal. Rev Investigaciones medicoquirúrgicas. 2009;1(2):5-12.

  27. Greenberg PD, Bertario L, Gnauck R, Kronborg O, Hardcastle JD, Epstein MS, et al. A prospective multicenter evaluation of new fecal occult blood tests in patients undergoing colonoscopy. The American Journal of Gastroenterology. 2000;95(5):1331-7.

  28. Hyun-Soo K, Kyu Hong K, Jin Heon H, Ki Won C, Jae Woo K, Hye Jung K, et al. Factors affecting the sensitivity or specificity of immunochemical fecal occult blood testing screening for colorectal neoplasia. Gastrointestinal Endoscopy. 2006;63(5):AB214.

  29. Dancourt V, Lejeune C, Lepage C, Gailliard MC, Meny B, Faivre J. Immunochemical faecal occult blood tests are superior to guaiac-based tests for the detection of colorectal neoplasms. Eur J Cancer. 2008;44(15):2254-8.

  30. Favennec L, Kapel N, Meillet D, Chochillon C, Gobert JG. Detection of occult blood in stools: comparison of three guaiac tests and a latex agglutination test. Ann Biol Clin. 1992;50:311-3.

  31. Goberts G. Comparison of two immunochemical methods for faecal occult blood detection. Clin Chem News. 1992;18(6):22-30.

  32. Guittet L, Bouvier V, Mariotte N, Vallee JP, Levillain R, Tichet T, et al. Comparison of a guaiac and an immunochemical faecal occult blood test for the detection of colonic lesions according to lesion type and location. British Journal of Cancer. 2009;100:1230-5.

  33. Castiglione G, Visioli CB, Ciatto S, Grazzini G, Bonanomi AG, Rubeca T, et al. Sensitivity of latex agglutination faecal occult blood test in the Florence District population-based colorectal cancer screening programme. Br J Cancer. 2007 June 4;96(11):1750-4.

  34. Diaz Tasende J, Marín Gabriel C. Cribado del cáncer colorrectal mediante test de sangre oculta en heces. Rev Esp Enferm Dig. 2008;100:315-9.

  35. Oort FA, Droste JS, Van der Hulst RW, Van Heukelem HA, Loffeld RJ, Wesdorp IC, et al. Colonoscopy-controlled intra-individual comparisons to screen relevant neoplasia: Faecal immunochemical test vs. Guaiac-based faecal occult blood test. Aliment Pharmacol Ther. 2010;31(3):432-9.

  36. Winamer SJ, Zauber AG. Prevention of colorectal cancer by colonoscopic polypectomy. The National Polyp Study Workgroup. N Engl J Med. 2005;5:329.

  37. Roque SF, Navarro LA. Prevención y pesquisa del cáncer colorrectal. Avances en la Gastroenterología en las Américas. Lima, Perú: Ed. Cimagraf; 2005. p. 168.

  38. Graser A, Stieber P, Nagel D, Schäfer C, Horst D, Becker CR, et al. Comparison of CT colonography, colonoscopy, sigmoidoscopy and faecal occult blood tests for the detection of advanced adenoma in an average risk population. Gut. 2009;58(2):241-8.

  39. Haug U. Immunochemical fecal blood tests differ widely in performance. Ann Intern Med. 2009;150:162-9.

  40. Tomás Moros M, García Durán F, Carneros Martín J, Sánchez Prudencio S, Villa Poza J, Ciriza de los Ríos J, et al. Neoplasia avanzada de colon. Estudio comparativo entre pacientes con antecedentes familiares de cáncer colorrectal y la población general. Gastroenterol Hepatol. 2009;32(3):234.

  41. Minami Y, Yoshikazu N. Increase of colon and rectal cancer incidence rates in Japan: Trends in incidence rates in Miyagi Prefecture, 1959-1997. Journal of Epidemiology. 2006;16(6):28-35.

  42. Hechevarría Borrero P, Meriño T. Características clínicas del cáncer de colon. Estudio de 57 pacientes. MEDISAN. 2003;7(3):4-9. (Consultado febrero 2009). Disponible en: http://bvs.sld.cu/revistas/san/vol7




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