Identification of human papillomavirus in benign and malignant nonmelanoma skin lesions by molecular methods

G Piccirilli; M Squeff; C Quattrocchi; R Fernández Bussy; D Chouhy; M Gorosito; A Sánchez; A Bergero; AA Giri; RA Fernández Bussy

2013, Number 3

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Med Cutan Iber Lat Am 2013; 41 (3)

Identification of human papillomavirus in benign and malignant nonmelanoma skin lesions by molecular methods

Piccirilli G, Squeff M, Quattrocchi C, Fernández BR, Chouhy D, Gorosito M, Sánchez A, Bergero A, Giri AA, Fernández BRA

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Language: Spanish
References: 21
Page: 97-102
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ABSTRACT

The human papillomavirus (HPV) establish a latent infection of the skin as normal flora and can develop skin lesions on some situations.
Objectives: Identify the HPV types which exhibit tropism for cutaneous epithelium and analyse its possible association with benign skin lesions and nonmelanoma skin cancer.
Materials and methods: Prospective study was carried out in the Department of Dermatology at the Centenario Provincial Hospital from June 2007 to November 2008. Samples were collected by swabs from the following areas: sun-exposed (FE), non-sun-exposed (NFE), perilesional (PL), lesion area (SL) and biopsy of lesions for histopathological study. We included patients referred to surgical removal of a skin lesion for histopathological study. Detection of HPV by PCR.
Results: 67 patients were recruited, 41 men and 26 women, median age of 51 years (range, 19-89 years). 300 samples were suitable for amplification by PCR. HPV DNA was present in 58% of the analyzed samples (176/300), and 75% in FE, 39% in NFE, 75% in PL, 66% in SL and 35% in biopsies. 69 different types of HPV were identified, being more frequent 2, 21, 20, and 6. We detected a new HPV, 115. HPV were not identified in samples of carcinomas. In seborrhoeic keratosis were present in 27% of the samples.
Conclusions: We collected data about the HPV circulating in patients of our region. We affirmed the influence of ultraviolet radiation on this viral infection, as well as their presence in seborrhoeic keratosis. We identified a new HPV in South America.

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