Rivero D, Kourí V, Correa C, Martínez MI, Llanes R, Gala Á, García E

Language: Spanish
References: 31
Page: 28-35
PDF size: 795.32 Kb.

ABSTRACT

Objective: To normalize two types of polymerase chain reaction (PCR) tests corresponding to the cryptic plasmid of Chlamydia trachomatis and evaluate its performance in endocervical exudate samples from women with lower abdominal pain.
Methods: Two simple PCR tests were normalized from the cryptic plasmid C. trachomatis (tests K and T) and their sensibilities were determined for strain L2 of C. trachomatis. Afterwards its performance was evaluated in 50 endocervical exudates samples in women with lower abdominal pain, from the municipality of “10 de Octubre”. Additionally, sensibility, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated for both tests, utilizing a major outer membrane protein (MOMP) PCR as a commercial real time reference.
Results: Both PCR tests showed good sensibility. With the K test 10 UFI/mL of the L2 strain of C. trachomatis were detected, while the T test showed a greater sensibility detecting less than 1 UFI/mL. Positive results were seen in 8% (4/50) of the samples tested by PCR in real time, as well as 6% (3/50) of the trials done using tests K and T. A sensibility of 75%, a specificity of 100%, a PPV of 100%, and a NPV of 97.9% were obtained with the cryptic plasmid PCR.
Conclusions: This study demonstrated that normalized PCR tests in our laboratory conditions provide trustworthy results for the diagnosis of C. trachomatis.

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