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Órgano oficial de la Facultad de Odontología, UNAM

2014, Number 3

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Rev Odont Mex 2014; 18 (3)

Candidate markers, culture strategies and DPSC perspectives used as cellular therapy in dentistry

Romero S, Córdoba K, Martínez VCA, Gutiérrez QJG, Durán RJY, Munévar NJC

Language: Spanish
References: 41
Page: 153-163
PDF size: 258.28 Kb.


ABSTRACT

Tissue engineering based on dental pulp stem cells is considered as a promising approach for regenerative dentistry. It purports the final target of morphologically and functionally replacing periodontal tissues and/or lost teeth by means of the in vitro synthesis of tissue-analog substitutes, or even a human tooth (called bio-tooth). Dental pulp stem cells represent a colony of adult cells which have the ability to auto-renovate and differentiate in different lineages. Dental pulp stem cells exact origin has yet to be fully determined; these stem cells seem to be the source of odontoblasts, which contribute to the formation of the dentin-pulp complex. Recently, achievements obtained through research conducted on stem cells, have allowed us to contemplate the possible therapeutic applications of dental pulp stem cells. Some studies have shown that dental pulp stem cells are able to produce in vivo dental tissues, including dental pulp and crown structures. Other research has demonstrated that these stem cells differentiate in vivo and in vitro into osteoblasts, neuroblasts, chondrocytes fibroblasts, and endothelium. In theory, a bio-tooth synthesized from autogenic dental pulp stem cells should be the best option to recover the whole structure and function of a human tooth. The aim of the present review article was to undertake a brief description of the location, origin, isolation and candidate markers of dental pulp stem cells in order to thus present application perspectives to be used in the dental clinic.


REFERENCES

  1. Huang AH, Snyder BR, Cheng PH, Chan AW. Putative dental pulp-derived stem/stromal cells promote proliferation and differentiation of endogenous neural cells in the hippocampus of mice. Troncales Cells. 2008; 26: 2654-2663.

  2. Ikeda E, Yagi K, Kojima M, Yagyuu T, Ohshima A, Sobajima S et al. Multipotent cells from the human third molar: feasibility of cell-based therapy for liver disease. Differentiation. 2008; 76: 495-505.

  3. Shi S, Gronthos S. Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. Journal of Bone and Mineral Research. 2003; 18: 696-704.

  4. Shi S, Robey PG, Gronthos S. Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone. 2001; 29: 532-539.

  5. Chai Y, Jiang X, Ito Y, Bringas P Jr, Han J, Rowitch D et al. Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis. Development. 2000; 127: 1671-1679.

  6. Clouthier DE, Williams SC, Yanagisawa H, Wieduwilt M, Richardson JA, Yanagisawa M. Signaling pathways crucial for craniofacial development revealed by endothelin-A receptor-deficient mice. Developmental Biology. 2000; 217: 10-24.

  7. Zhang J, Duan X, Zhang H, Deng Z, Zhou Z, Wen N et al. Isolation of neural crest-derived stem cells from ratembryonic mandibular processes. Biology of the Cell. 2006; 98: 567-575.

  8. Yan M, Yu Y, Zhang G, Tang C, Yu J. A journey from dental pulp stem cells to a bio-tooth. Stem Cell Rev. 2011; 7: (1) 161–171.

  9. Friedenstein AJ. Precursor cells of mechanocytes. Int Rev Cytol. 1976; 47: 327-359.

  10. Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 1970; 3: 393-403.

  11. Friedenstein AJ. Stromal mechanisms of bone marrow: cloning in vitro and retransplantation in vivo. Haematol. Blood Transfus. 1980; 25: 19-29.

  12. Huang GT, Sonoyama W, Chen J, Park SH. In vitro characterization of human dental pulp cells: various isolation methods and culturing environments. Cell Tissue Res. 2006; 324: 225-236.

  13. Wiesmann A, Buhring HJ, Mentrup C, Wiesmann HP. Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation. Head Face Med. 2006; 31: 2-8.

  14. Duff S, Li C, Garland J, Kumar S. CD105 is important for angiogénesis: evidence and potential applications. The FASEB Journal. 2003; 17 (9): 982-992.

  15. Gonçalves R, da Silva C, Cabra J, Zanjani E, Almeida-Porada G. STRO-1+ human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture sytroncales. Exp Hematol. 2006; 34: 1353-1359.

  16. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000; 97 (25): 13625-13630.

  17. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG et al. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A. 2003; 100 (10): 5807-5812.

  18. Yu J, Wang Y, Deng Z, Tang L, Li Y, Shi J et al. Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. Biology of the Cell. 2007; 99: 465-474.

  19. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A et al. Stem cell properties of human dental pulp stem cells. J Dent Res. 2002; 81: 531-535.

  20. Yu JH, Deng ZH, Shi JN, Zhai HH, Nie X, Zhuang H et al. Differentiation of dental pulp stem cells into regular-shaped dentin-pulp complex induced by tooth germ cell conditioned medium. Tissue Engineering. 2006; 12: 3097-3105.

  21. Nakashima M, Mizunuma K, Murakami T, Akamine A. Induction of dental pulp stem cell differentiation into odontoblasts by electroporation-mediated gene delivery of growth/differentiation factor 11 (Gdf11). Gene Therapy. 2002; 9: 814-818.

  22. El-Backly RM, Massoud AG, El-Badry AM, Sherif RA, Marei MK. Regeneration of dentine/pulp-like tissue using a dental pulp stem cell/poly (lactic-co-glycolic) acid scaffold construct in New Zealand white rabbits. Australian Endodontic Journal. 2008; 34: 52-67.

  23. Batouli S, Miura M, Brahim J, Tsutsui TW, Fisher LW, Gronthos S et al. Comparison of stem-cell-mediated osteogenesis and dentinogenesis. J Dent Res. 2013; 82 (12): 976-981.

  24. Duailibi MT, Duailibi SE, Young CS, Bartlett JD, Vacanti JP, Yelick PC. Bioengineered teeth from cultured rat tooth bud cells. J Dent Res. 2004; 83: 523-528.

  25. Nakao K, Morita R, Saji Y, Ishida K, Tomita Y, Ogawa M et al. The development of a bioengineered organ germ method. Nature Methods. 2007; 4: 227-230.

  26. Ikeda E, Morita R, Nakao K, Ishida K, Nakamura T, Takano-Yamamoto T et al. Fully functional bioengineered tooth replacement as an organ replacement therapy. Proc Natl Acad Sci U S A. 2009; 106: 13475-13480.

  27. Yu JH, Jin F, Deng ZH, Li YF, Tang L, Shi JN et al. Epithelial-mesenchymal cell ratios can determine the crown morphogenesis of dental pulp stem cells. Stem Cells and Development. 2008; 17: 475-482.

  28. Yu J, Shi J, Jin Y. Current approaches and challenges in making a bio-tooth. Tissue Eng Part B Rev. 2008; 14: 307-319.

  29. Yan X, Qin H, Qu C, Tuan RS, Shi S, Huang GT. iPS cells reprogrammed from human mesenchymal-like stem/progenitor cells of dental tissue origin. Stem Cells and Development. 2010; 19: 469-480.

  30. Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature. 2007; 448: 313-317.

  31. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007; 131: 861-872.

  32. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007; 318: 1917-1920.

  33. Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T et al. Generation of induced pluripotent stem cells without myc from mouse and human fibroblasts. Nat Biotechnol. 2008; 26: 101-106.

  34. Rubio D, García-Castro J, Martín MC, de la Fuente R, Cigudosa JC, Lloyd AC et al. Spontaneous human adult stem cell transformation. Cancer Res. 2005; 65 (8): 3035-3039.

  35. Kanematsu A, Yamamoto S, Ozeki M, Noguchi T, Kanatani I, Ogawa O et al. Collagenous matrices as release carriers of exogenous growth factors. Biomaterials. 2004; 25: 4513-4520. [PubMed: 15046942]

  36. Stiver SI, Tan X, Brown LF, Hedley-Whyte ET, Dvorak HF. VEGF-A angiogenesis induces a stable neovasculature in adult murine brain. J Neuropathol Exp Neurol. 2004; 63: 841-55. [PubMed: 15330339]

  37. Nosrat IV, Smith CA, Mullally P, Olson L, Nosrat CA. Dental pulp cells provide neurotrophic support for dopaminergic neurons and differentiate into neurons in vitro; implications for tissue engineering and repair in the nervous system stem cells. Eur J Neurosci. 2004; 19: 2388-2398. [PubMed: 15128393]

  38. Kolf C, Cho E, Tuan R. Mesenchymal stromal cells. Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation. Arthritis Res Ther. 2007; 9: 204.

  39. Huang GTJ, Yamaza T, Shea LD, Djouad F, Kuhn NZ, Tuan RS et al. Stem/progenitor cell–mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model. Tissue Engineering Part A. 2010; 16: 605-615. [PubMed: 19737072]

  40. Fan Y, Sun Z, Wang R, Abbott C, Moradian-Oldak J. Enamel inspired nanocomposite fabrication through amelogeninsupramolecular assembly. Biomaterials. 2007; 28: 3034-3042. [PubMed: 17382381]

  41. Yin Y, Yun S, Fang J, Chen H. Chemical regeneration of human tooth enamel under nearphysiological conditions. Chem Commun (Camb). 2009; (39): 5892-5894. [PubMed: 19787132]




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