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2011, Number 2

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RCAN 2011; 21 (2)

Efecto antianémico y daño gastrointestinal en ratas suplementadas con diferentes formas de hierro

García HY, González HR, Espinosa VG, Carmona CA, Cárdenas VR

Language: Spanish
References: 27
Page: 186-196
PDF size: 296.06 Kb.


ABSTRACT

The Cuban National Center for Biopreparations has developed a line of de anti-anemics obtained from bovine blood which comprises Trofin oral solution and NeoTrofin® and NeoTrofin® tablets. The efficacy of such anti-anemics is higher than 90%, and no adverse reactions have been reported after their use. Treatment of anemia has traditionally relied upon use of suplements incorporating ionic iron and folic acid. Intestinal absorption of iron is mediated by two different receptors: DMT1 for the ionic form and HCP1 for the heme one. Anti-anemic effect of preparations of iron salts and Trofin® formulations could be promoted by means of simultaneous stimulation of the described routes of absorption, thus using lower dose of the mineral, resulting in a diminished rate of nondesirable gastrointestinal effects. Anemic Wistar rats were distributed in three groups, and each group where supplemented with Trofin® (Diet A), Iron Sulphate (Diet B), and Trofin® + Iron Sulphate (Diet C), respectively, during 10 days. The three diets showed a similar anti-anemic effect, confirming the bioavailability of iron after joint administration of ionic and heme forms, and hence, the superior effectiveness of Diet C. Used diets were also similar in the occurrence of non-desirable gastrointestinal effects, suggesting that examination of stomach and duodenum could serve to assess safety and tolerance of future anti-anemic preparations.


REFERENCES

  1. McLean E, Egli I, Benoist B, Wojdyla D, Cogswell M. Worldwide prevalence of anemia in preschool aged children, pregnant women and non-pregnant women of reproductive age. En: Nutritional anemia (Editores: Kraemer K, Zimmermann MB). Sight and Life Press. Switzerland: 2007. pp 3.

  2. Vitaminas y minerals: Hierro. Aparecido en: http://www.nutrinet.org. Disponible en: http://cuba.nutrinet.org/areastematicas/ vitaminas-yminerales/ introduccion/117?task=view Fecha de última visita: 23 de julio del 2010.

  3. González R, Aznar E, González M. Composición físico-química del reconstituyente y antianémico Trofín®. Rev Mex Cien Farm 2004;36:2-5.

  4. Aznar E, González R, Moroño M, González M. Tratamiento anti-anémico Fe-proteína (Trofin) para uso pediátrico. Rev Mex Cienc Farm 1998;29:18-21.

  5. Aznar, E, González R, González M. Ensayo clínico (Fase II) en embarazadas de nueva forma farmacéutica de Trofin en tabletas (NeoTrofin® y NeoTrofinC®). Av Biotec Mod 2003;5.

  6. González R, Aznar E, González M, Hernández JC, Varela A, Silva P, García Y. Nueva línea de productos para prevenir y tratar la anemia partiendo del hierro hemínico. Informacéutico 2008; 15:43-8.

  7. Hurrell R. Preventing iron deficiency through food fortification. Nutr Rev 1999;55:210-22.

  8. Venkatesh M. The case for urgent action to address nutritional anemia. En: Nutritional anemia (Editores: Kraemer K, Zimmermann MB). Sight and Life Press. Switzerland: 2007. pp 13.

  9. Lund, EK, Fairweather S, Wharf G, Johnson IT. Chronic exposure to high levels of dietary iron fortification increases lipid peroxidation in the mucosa of the rat large intestine. J Nutr 2001;131:2928-31.

  10. Olivares GM. Suplementación con Fe. Rev Chil Nutr 2004;31(3). Disponible en: http://www.scielo.cl/scielo.php?script=s ci_arttext&pid=S0717- 75182004000300001&tlng=en&lng=en &nrm=iso. Fecha de última visita: 25 de Marzo del 2011.

  11. Alderman H, Horton S. The economics addressing nutritional anemia. Nutritional anemia (Editores: Kraemer K, Zimmermann MB). Sight and Life Press. Switzerland: 2007. pp 3.

  12. Conrad ME, Umbriet JN. Pathways of iron absorption. Blood Cel Mol Dis 2002;29:336-55.

  13. Fleming MD, Romano MA, Su MA, Garrick LM, Garrick MD, Andrews NC. Nramp 2 is mutated in the anemic Belgrade (b) rat: Evidence of a role for Nramp 2 in endosomal iron transport. Proc Natl Acad Sci USA 1998;95:1148- 53.

  14. Shayeghi M, Latunde-Dada GO, Cahill JS, Laftah AH, Takeuchi K, Halliday N et al. Identification of an intestinal heme transporter. Cell 2005;122:789-801.

  15. Carpenter CE y Mahoney AW. Contributions of heme and nonheme iron to human nutrition. Food Sci Nutr 1992; 31:333-67.

  16. Shufa D, Flegying Z, Youfa W, Barry MP. Current methods for estimating dietary iron bioavailability do not work in China. J Nutr 2000;130:193-8.

  17. Pizarro F, Olivares M, Hertrampf E, Mazariegos DI, Arredondo M, Letelier A et al. Iron bis-glycine chelate competes for the nonheme-iron absorption pathway. Am J Clin Nutr 2002;76:577-81.

  18. García Hernández Y, González Hernández R, Cárdenas Vázquez R, Carmona Castro A. Desarrollo de un biomodelo de ratas anémicas mediante dos tipos de dietas de caseína. RCAN Rev Cubana Aliment Nutr 2010;20:26- 34.

  19. Benito P, House W y Millar D. Influence of iron supplementation frecuency on Absorption efficiency and mucosal ferritin in anaemic rats. Brit J Nutr 1997; 78:469-77.

  20. Knutson MD, Walter PB, Ames BN, Viteri FE. Both iron deficiency and daily iron supplements increased lipid peroxidation in rats. J Nutr 2000; 130:261-8.

  21. Drabkin DL, Austin JH. Spectrophotometric studies: V. A technique for the analysis of undiluted blood and concentrated hemoglobin solutions. J Biol Chem 1935;112:105-15.

  22. Barry M, Sherlock S. Measurement of liver iron concentration in needle biopsy specimens. Lancet 1971; i:100-3.

  23. Srigiridhar K, Nair KM Iron deficient intestine is more susceptible to peroxidation damage during iron supplementation in rats. Free Radic Biol Med 1998;25:660-5.

  24. Okhawa O, Yagi K. Assay of lipid peroxides in animal tissues by the thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.

  25. Reznick AZ, Packer L. Oxidative damage to proteins: Spectrophometric method for carbonyl assay. Methods of Enzymology 1994;233:357-63.

  26. Hernández M, Sousa V, Moreno A, Villalpando S, López Alarcón M. Iron bioavailability and utilization in rats are lower from lime-treated corn flour than from wheat flour when they are fortified with different source of iron. J Nutr 2003;133:154-9.

  27. Anónimo. Nutrient Requirements of laboratory rat. Subcommittee on Laboratory Animal Nutrition. En: Nutrient Requirements of laboratory animals. Academy Press. Washington: 1995. pp 11-79.




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