García HY, García-Pérez Á, Ángeles-Campos SC, Carmona-Castro A, Cárdenas-Vázquez R

Language: Spanish
References: 14
Page: 312-320
PDF size: 163.99 Kb.

ABSTRACT

Introduction: the scientific and technological development of biological and biomedical sciences has been possible due to the use of experimental animal models. Iron- (Fe-) deficiency anemia affects about one third of the world population. Just weaned rats have been the animal model most commonly used to obtain an experimental biomodel of anemia. The disease is obtained by the hemoglobin (Hb) depletion method, feeding the animal an iron-deficient diet.
Objective: evaluate the efficacy of a purified diet based on casein to obtain anemic rats.
Method: a study was conducted of 40 just weaned (21-24 days) Sprague Dawley rats, 20 of each sex. The animals were housed in groups of 3 rats of the same sex in stainless steel boxes with mesh bottoms. For 8 weeks the animals were administered deionized water and a casein diet of 12.58% protein and 1.28 mgFe/kg. Body weight was measured every two weeks. Hb concentration was gauged from the fourth week onwards.
Results: a significant increase in body weight was obtained, with no differences between male and female rats. The criterion for anemia was at least a 30% decrease in Hb with respect to the average baseline value per group. The percentage of anemic animals at 8 weeks was higher for females (75%) than for males (60%).
Conclusions: the diet used for the study made it possible to obtain female anemic rats with acceptable efficiency in relation to the percentage of anemic animals and the increase in body weight.

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