medigraphic.com
ISSN 1561-3011 (Electronic)

2013, Number 3

<< Back Next >>

Rev Cubana Invest Bioméd 2013; 32 (3)

Evaluation of the cytotoxicity of Clindamycin in cultures of gingival human fibroblasts

Cabrales SR, Kanako YA, Díaz CA, Prokopowitsch I

Language: Spanish
References: 30
Page: 302-311
PDF size: 212.66 Kb.


ABSTRACT

Introduction: the presence of residual microorganisms after chemical-surgical preparation of root canals suggests the use of effective intracanal medication, biologically compatible with periapical tissues.
Objective: compare the cytotoxicity of intracanal medications in gingival human fibroblasts.
Methods: an experimental study was conducted with four different groups: control (G1), Clindamycin Hydrochloride 2g associated to dexamethasone phosphate 0.32g (Clindex) vehicled in alcoholic solution (G2), Clindex vehicled in polyethylene glycol 400 (G3), and NDP: paramonochlorophenol associated to dexamethasone phosphate 0.32g vehicled in polyethylene glycol 400 and saline solution (G4). The cells were stimulated with the medications for 24, 48 and 72 hours. Cell viability was evaluated with the technique of MTT analysis. Readings were performed in an ELISA spectrophotometer.
Results: the groups were analyzed in terms of mitochondrial activity. G2 had the best results in the three times studied, followed by G3 and G4. A statistical analysis was performed (ANOVA and complemented by Tukey's test). Significant differences of 1% were found when comparing G2 and G3 with G4.
Conclusion: the Clindamycin dexamethasone combination, irrespective of the vehicle, showed greater cell viability than paramonochlorophenol associated to dexamethasone phosphate.


REFERENCES

  1. Rodriguez-Nunez A, Cisneros-Cabello R, Velasco-Ortega E, Llamas-Carreras JM, Torres-Lagares D, Segura-Egea JJ. Antibiotic use by members of the Spanish Endodontic Society. J Endod. 2009;35(9):1198-1203.

  2. Segura-Egea JJ, Velasco-Ortega E, Torres-Lagares D, Velasco-Ponferrada MC, Monsalve-Guil L, Llamas-Carreras JM. Pattern of antibiotic prescription in the management of endodontic infections amongst Spanish oral surgeons. Int Endod J. 2010;43(4):342-50.

  3. Parmar D, Hauman CH, Leichter JW, McNaughton A, Tompkins GR. Bacterial localization and viability assessment in human ex vivo dentinal tubules by fluorescence confocal laser scanning microscopy. Int Endod J. 2011;44(7):644-51.

  4. Jung DJ, Al-Ahmad A, Follo M, Spitzmuller B, Hoth-Hannig W, Hannig M, et al. Visualization of initial bacterial colonization on dentine and enamel in situ. J Microbiol Methods. 2010;81(2):166-74.

  5. Puglisi S, Privitera S, Maiolino L, Serra A, Garotta M, Blandino G, et al. Bacteriological findings and antimicrobial resistance in odontogenic and nonodontogenic chronic maxillary sinusitis. J Med Microbiol. 2011;60(Pt 9):1353-9.

  6. LeCorn DW, Vertucci FJ, Rojas MF, Progulske-Fox A, Belanger M. In vitro activity of amoxicillin, clindamycin, doxycycline, metronidazole, and moxifloxacin against oral Actinomyces. J Endod. 2007;33(5):557-60.

  7. Molander A, Reit C, Dahlen G. Microbiological evaluation of clindamycin as a root canal dressing in teeth with apical periodontitis. Int Endod J. 1990;23(2):113-8.

  8. Mohammadi Z, Abbott PV. On the local applications of antibiotics and antibiotic-based agents in endodontics and dental traumatology. Int Endod J. 2009;42(7):555-67.

  9. Gilad JZ, Teles R, Goodson M, White RR, Stashenko P. Development of a clindamycin-impregnated fiber as an intracanal medication in endodontic therapy. J Endod. 1999;25(11):722-7.

  10. Lin S, Levin L, Peled M, Weiss EI, Fuss Z. Reduction of viable bacteria in dentinal tubules treated with clindamycin or tetracycline. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96(6):751-6.

  11. Bratka-Robia CB, Mitteregger G, Aichinger A, Egerbacher M, Helmreich M, Bamberg E. Primary cell culture and morphological characterization of canine dermal papilla cells and dermal fibroblasts. Vet Dermatol. 2002;13(1):1-6.

  12. Danisovic L, Varga I, Polak S, Ulicna M, Bohmer D, Vojtassak J. Morphology of in vitro expanded human muscle-derived stem cells. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2008;152(2):235-8.

  13. Leik CE, Willey A, Graham MF, Walsh SW. Isolation and culture of arterial smooth muscle cells from human placenta. Hypertension. 2004;43(4):837-40.

  14. Fadda S, Cincotti A, Cao G. A novel population balance model to investigate the kinetics of in vitro cell proliferation: Part I. model development. Biotechnol Bioeng. 2012;109(3):772-81.

  15. Linder B, Bostrom M, Gerdin B, Rask-Andersen H. In vitro growth of human endolymphatic sac cells: a transmission electron microscopic and immunohistochemical study in patients with vestibular schwannoma and Meniere's disease. Otol Neurotol. 2001;22(6):938-43.

  16. Asakawa N, Shimizu T, Tsuda Y, Sekiya S, Sasagawa T, Yamato M, et al. Pre-vascularization of in vitro three-dimensional tissues created by cell sheet engineering. Biomaterials. 2010;31(14):3903-9.

  17. Soos K, Valkusz Z, Vetro O, Juhasz A, Petri A, Molnar G, et al. Primary mono-layer cell cultures as model system for studying of environmental toxic agents: organochlorine compounds. Cent Eur J Public Health. 2000;8 Suppl:35-6.

  18. Sardi JC, Duque C, Mariano FS, Marques MR, Hofling JF, Goncalves RB. Adhesion and invasion of Candida albicans from periodontal pockets of patients with chronic periodontitis and diabetes to gingival human fibroblasts. Med Mycol. 2012;50(1):43-9.

  19. Akerud A, Dubicke A, Sennstrom M, Ekman-Ordeberg G, Malmstrom A. Differences in heparan sulfate production in cervical fibroblast cultures from women undergoing term and preterm delivery. Acta Obstet Gynecol Scand. 2008;87(11):1220-8.

  20. Hager B, Bickenbach JR, Fleckman P. Long-term culture of murine epidermal keratinocytes. J Invest Dermatol. 1999;112(6):971-6.

  21. Malkoc S, Corekci B, Ulker HE, Yalcin M, Sengun A. Cytotoxic effects of orthodontic composites. Angle Orthod. 2010;80(4):571-6.

  22. Ng YL, Mann V, Gulabivala K. A prospective study of the factors affecting outcomes of nonsurgical root canal treatment: part 1: periapical health. Int Endod J. 2011;44(7):583-609.

  23. Contardo L, Meneguzzi E, Cadenaro M, Di Lenarda R. Clinical evaluation of antibiotic prophylaxis before endodontic treatment of necrotic teeth. Minerva Stomatol. 2005;54(3):153-60.

  24. Trairatvorakul C, Detsomboonrat P. Success rates of a mixture of ciprofloxacin, metronidazole, and minocycline antibiotics used in the non-instrumentation endodontic treatment of mandibular primary molars with carious pulpal involvement. Int J Paediatr Dent. 2012 May;22(3):217-27. doi: 10.1111/j.1365- 263X.2011.01181.x.

  25. Mohammadi Z. Systemic, prophylactic and local applications of antimicrobials in endodontics: an update review. Int Dent J. 2009;59(4):175-186.

  26. Osorio RM, Hefti A, Vertucci FJ, Shawley AL. Cytotoxicity of endodontic materials. J Endod. 1998;24(2):91-96.

  27. Davis RR, Lockwood PE, Hobbs DT, Messer RL, Price RJ, Lewis JB, et al. In vitro biological effects of sodium titanate materials. J Biomed Mater Res B Appl Biomater. 2007;83(2):505-11.

  28. Collier AC, Pritsos CA. The mitochondrial uncoupler dicumarol disrupts the MTT assay. Biochem Pharmacol. 2003;66(2):281-7.

  29. Wijsman JA, Dekaban GA, Rieder MJ. Differential toxicity of reactive metabolites of clindamycin and sulfonamides in HIV-infected cells: influence of HIV infection on clindamycin toxicity in vitro. J Clin Pharmacol. 2005;45(3):346-51.

  30. Duewelhenke N, Krut O, Eysel P. Influence on mitochondria and cytotoxicity of different antibiotics administered in high concentrations on primary human osteoblasts and cell lines. Antimicrob Agents Chemother. 2007;51(1):54-63.




2020     |     www.medigraphic.com