medigraphic.com
ISSN 1561-3011 (Electronic)

2013, Number 3

<< Back Next >>

Rev Cubana Invest Bioméd 2013; 32 (3)

Immunogenicity of recombinant proteins compared with the use of bioinformatics tools

Serrano BOR

Language: Spanish
References: 27
Page: 293-301
PDF size: 71.52 Kb.


ABSTRACT

Objective: Determine the usefulness of immunoinformatics tools to detect potentially immunodominant peptides, and evaluate the differences between the immune responses provided by the animal models used in preclinical and human studies.
Methods: Modeling was conducted of the response to two exogenous proteins: recombinant streptokinase and hepatitis B surface antigen. Based on their primary sequences, algorithms were used to identify B and T epitopes against HLA class I and II molecules (HLA-A*0201, HLA-DRB1*0301 and HLA-DRB1*0701), and murine haplotypes H2-Kd and H2-Kk. The highest scoring peptides were chosen.
Results: ABCPred algorithm showed a better prediction capacity for B epitopes, whereas coincidence was greater in modeling programs for the T response. The epitopes generated for haplotype H2-Kk had greater similitude with those presented by the HLA molecules selected.
Conclusions: A methodology is presented which is applicable to the development of subunit and multiepitope vaccines, as well as other peptidic biotechnological drugs. This methodology allows optimization of the preclinical and clinical phases at a very low cost, with minimal technological requirements, optimal use of media, and resources and human capital available at any institution of the national health system.


REFERENCES

  1. Bodhade AS, Ganvir SM, Hazarey VK. Oral manifestations of HIV infection and their correlation with CD4 count. J Oral Sci. 2011 Jun;53(2):203-11.

  2. Pinzón E, Bravo S, Méndez F, Clavijo G, León M. Prevalencia y factores relacionados con la presencia de manifestaciones orales en pacientes con VIH/SIDA, Cali, Colombia. Colomb Med. 2008;39(4): 346-55.

  3. Sharma G, Pai KM, Suhas S, Ramapuram JT, Doshi D, Anup N. Oral manifestations in HIV/AIDS infected patients from India. Oral Dis. 2006 Nov;12(6):537-42.

  4. Ranganathan K, Hemalatha R. Oral lesions in HIV infection in developing countries: an overview. Adv Dent Res. 2006 Apr 1;19(1):63-8.

  5. Gileva OS, Sazhina MV, Gileva ES, Efimov AV, Scully C. Spectrum of oral manifestationsof manifestations of HIV/AIDS in the Perm region (Russia) and identification of self-induced ulceronecrotic lingual lesions. Med Oral. 2004 May;9(3):212-5.

  6. Patton LL, Phelan JA, Ramos-Gomez FJ, Nittayananta W, Shiboski CH, Mbuguye TL. Prevalence and classification of HIV-associated oral lesions. Oral Dis. 2002;8 Suppl 2:98-109.

  7. Coogan M, Greenspan J, Challacombe SJ. Oral lesions in infection with human immunodeficiency virus. Bull World Health Organ. 2005; 83(9):700-6.

  8. Reznik DA. Oral manifestations of HIV disease. Top HIV Med. 2005 Dec-2006 Jan;13(5):143-8.

  9. Nokta M. Oral manifestations associated with HIV infection. Curr HIV/AIDS Rep. 2008 Feb;5(1):5-12.

  10. Jané-Salas E, Chimenos-Küstner E, López-López J, Roselló-Llabrés X, Ocaña -Rivera I. Efecto de los tratamientos antirretrovirales en las manifestaciones orales de los pacientes VIH+. Av. Odontoestomatol. 2006; 22(6): 315-26.

  11. Ortega KL, Vale DA, Magalhães MH. Impact of PI and NNRTI HAART-based therapy on oral lesions of Brazilian HIV-infected patients. J Oral Pathol Med. 2009 Jul;38(6):489-94.

  12. Kakabadze T, Rukhadze N, Mshvidobadze K, Lomtadze M, Kandelaki G. Oral lesions in HIV-positive patients in Georgia. Georgian Med News. 2008 Dec;(165):60-5.

  13. Owotade FJ, Shiboski CH, Poole L, Ramstead CA, Malvin K, Hecht FM, Greenspan JS. Prevalence of oral disease among adults with primary HIV infection. Oral Dis. 2008 Sep;14(6):497-9.

  14. Mellors J, Muñoz A, Giorgi J, Margolick J, Tassoni C, y otros. et al. Plasma viral load and CD4+ lymphocytes as prognostic markers of HIV-1 infection. Ann Intern Med. 1997 Jun 15;126(12):946-54.

  15. Rodríguez M. Manifestaciones orales asociadas con la infección por VIH-SIDA. Rev Cub Estomatol. 2005 Ene 42;(1):1-6.

  16. Ranganathan K, Umadevi M, Saraswathi TR, Kumarasamy N, Solomon S, Johnson N. Oral lesions and conditions associated with human immunodeficiency virus infection in 1000 South Indian patients. Ann Acad Med Singapore. 2004 Jul;33(4):37-42.

  17. Lourenço AG, Figueiredo LT. Oral lesions in HIV infected individuals from Ribeirão Preto, Brazil. Med Oral Patol Oral Cir Bucal. 2008 May 1;13(5):281-6.

  18. Bravo IM, Correnti M, Escalona L, Perrone M, Brito A, Tovar V, Rivera H. Prevalence of oral lesions in HIV patients related to CD4 cell count and viral load in a Venezuelan population. Med Oral Patol Oral Cir Bucal. 2006 Jan 1;11(1):33-9.

  19. Ramírez V, Esquivel L, Irigoyen E, Anaya G, González I. Association of oral lesions with HIV serological status. Salud Publica Mex. 2002 Mar-Apr;44(2):87-91.

  20. Benito M, Benito UM, Bernardoni C, Arteaga M, Sotolongo M, Benito M, Pereira S, Morón A. Manifestaciones bucales en pacientes VIH positivos y su relación con valores de Linfocitos CD4. Acta Odontol. Venez. 2007 45;(2):229-33.

  21. Ceballos-Salobreña A, Gaitaín-Cepeda L, Ceballos-García L, y otros.et al. Prevalence of oral lesions by Candida sp: Their varieties and serotypes in a population of patients with AIDS under a highly active antiretroviral therapy. Rev Iberoam Micol. 1998 Jul; 15: 141-5.

  22. Kamiru HN, Naidoo S. Oral HIV lesions and oral health behaviour of HIV-positive patients attending the Queen Elizabeth II Hospital, Maseru, Lesotho. SADJ. 2002 Dec;57(11):479-82.

  23. Guteta S, Feleke Y, Fekade D, Neway M, Diro E. Prevalence of oral and perioral manifestations in HIV positive adults at Tikur Anbessa Teaching Hospital Addis Ababa, Ethiopia. Ethiop Med J. 2008 Oct;46(4):349-57.

  24. Tappuni AR y & Fleming GJ. The effect of antiretroviral therapy on the prevalence of oral manifestations in HIV-infected patients: a UK study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 Dec;92(6):623-8.

  25. Margiotta V, Campisi G, Mancuso S, Accurso V, Abbadessa V. HIV infection: oral lesions, CD4+ cell count and viral load in an Italian study population. J Oral Pathol Med. 1999 Apr;28(4):173-7.

  26. Baqui A, Meiller T, Jabra-Rizk M, Zhang M, Kelley J, Falkler W. Association of HIV viral load with oral diseases. Oral Dis. 1999 Oct;5(4):294-8.

  27. Nicolatou-Galitis O, Velegraki A, Paikos S, Economopoulou P, Stefaniotis T, Papanikolaou IS, Kordossis T. Effect of PI-HAART on the prevalence of oral lesions in HIV-1 infected patients. A Greek study. Oral Dis. 2004 May;10(3):145-50.




2020     |     www.medigraphic.com