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2014, Number 1

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Cir Cir 2014; 82 (1)

Effect of cryotherapy over the expression of vascular endothelial growth factor and pigment epithelium-derived factor

Toscano-Garibay JD, Quiroz-Mercado H, Espitia-Pinzón C, Gil-Carrasco f, Flores-Estrada JJ

Language: Spanish
References: 40
Page: 3-10
PDF size: 436.30 Kb.


ABSTRACT

Background: Cryotherapy is a no invasive technique that uses intense cold to freeze and destroy cancer tissues. There are no descriptions of its effects over the expression of vascular endothelial growth factor and pigment epithelium-derived factor.
Methods: Experimental study in cryogenic spot were applied in the right sclera of twelve pigs for ten minutes. Other 3 pigs were used as normal controls. Animals were sacrificed at 7, 14 and 21 and the tissues of choriodes and retina were dissected in areas of approximately 1 cm2 surrounding cryogenic spots. Expression levels of vascular endothelial growth factor and pigment epithelium-derived factor were determined analyzed using polymerase chain reaction coupled to reverse-transcription.
Results: Vascular endothelial growth factor was significantly downregulated (24%, p ‹ 0.05) seven days post-treatment meanwhile pigment epithelium-derived factor levels increased 44.8% (p ‹ 0.05) as compared to normal controls (untreated). Both vascular endothelial growth factor and pigment epithelium-derived factor levels remain the same until day 14 but returned to basal expression at day 21.
Discussion: This work expose the relation of cryotherapy with the expression of two factors related to angiogenesis. Results showed significant changes on the expression of vascular endothelial growth factor and pigment epithelium-derived factor illustrating that both proteins are regulated in response to cryogenic treatment in relatively short periods (21 days).


REFERENCES

  1. Murukesh N, Dive C, Jayson GC. Biomarkers of angiogenesis and their role in the development of VEGF inhibitors. Br J Cancer 2010;102:8-18.

  2. Raica M, Cimpean AM. Platelet-Derived Growth Factor (PDGF)/PDGF Receptors (PDGFR) Axis as Target for Antitumor and Antiangiogenic Therapy. Pharmaceuticals 2010;3:572-599.

  3. Wang F, Rendahl KG, Manning WC, Quiroz D, Coyne M, Miller SS. AAV-Mediated Expression of Vascular Endothelial Growth Factor Induces Choroidal Neovascularization in Rat. Invest Ophthalmol Vis Sci 2003;44:781-790.

  4. Sivalingam A, Kenney J, Brown GC, Benson WE, Donoso L. Basic Fibroblast Growth Factor Levels in the Vitreous of Patients with Proliferative Diabetic Retinopathy. Arch Ophthalmol 1990;108:869-872.

  5. Grant M, Russell B, Fitzgerald C, Merimee TJ. Insulin-Like Growth Factors in Vitreous. Studies in Control and Diabetic Subjects with Neovascularization. Diabetes 1986;35:416- 420.

  6. Meyer-Schwickerath R, Pfeiffer A, Blum WF, Freyberger H, Klein M, Lösche C, et al. Vitreous levels of the insulin-like growth factors I and II, and the insulin-like growth factor binding proteins 2 and 3, increase in neovascular eye disease. Studies in nondiabetic and diabetic subjects. J Clin Invest 1993;92:2620-2625.

  7. Elner SG, Strieter R, Bian ZM, Kunkel S, Mokhtarzaden L, Johnson M, et al. Interferon-Induced Protein 10 and Interleukin 8 C-X-C Chemokines Present in Proliferative Diabetic Retinopathy. Arch Ophthal 1998;116:1597- 1601.

  8. Yoshida A, Yoshida S, Khalil AK, Ishibashi T, Inomata H. Role of NF-kappaB-mediated interleukin-8 expression in intraocular neovascularization. Invest Ophthal Vis Sci 1998;39:1097-1106.

  9. Khaliq A, Foreman D, Ahmed A, Weich H, Gregor Z, McLeod D, et al. Increased expression of placenta growth factor in proliferative diabetic retinopathy. Labor Invest J Technical Methods Pathol 1998;78:109-116.

  10. Katsura Y, Okano T, Noritake M, Kosano H, Nishigori H, Kado S, et al. Hepatocyte Growth Factor in Vitreous Fluid of Patients With Proliferative Diabetic Retinopathy and Other Retinal Disorders. Diabetes Care 1998;21:1759-1763.

  11. Hellstrom A, Perruzzi C, Ju M, Engström E, Hård AL, Liu JL, et al. Low IGF-I suppresses VEGF-survival signaling in retinal endothelial cells: Direct correlation with clinical retinopathy of prematurity. Proc Natl Acad Sci USA 2001;98:5804-5808.

  12. Lashkari K, Hirose T, Yazdany J, McMeel JW, Kazlauskas A, Rahimi N. Vascular Endothelial Growth Factor and Hepatocyte Growth Factor Levels are Differentially Elevated in Patients with Advanced Retinopathy of Prematurity. Am J Pathol 2000;156:1337-1344.

  13. Pierce EA, Foley ED, Smith LE. Regulation of Vascular Endothelial Growth Factor by Oxygen in a Model of Retinopathy of Prematurity. Arch Ophthal 1996;114:1219-1228.

  14. Tischer E, Mitchell R, Hartman T, Silva M, Gospodarowicz D, Fiddes JC, et al. The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. J Bio Chem 1991;266:11947- 11954.

  15. Yoo MH, Hyun HJ, Koh JY, Yoon YH. Riluzole Inhibits VEGF-Induced Endothelial Cell Proliferation In Vitro and Hyperoxia-Induced Abnormal Vessel Formation In Vivo. Invest Ophthalmol and Vis Sci 2005;46:4780-4787.

  16. Ferrari G, Pintucci G, Seghezzi G, Hyman K, Galloway AC, Mignatti P. VEGF, a Prosurvival Factor, Acts in Concert with TGF-beta1 to Induce Endothelial Cell Apoptosis. Proc Natl Acad Sci USA 2006;103:17260-17265.

  17. Distler JH, Hirth A, Kurowska-Stolarska M, Gay RE, Gay S, Distler O. Angiogenic and angiostatic factors in the molecular control of angiogenesis. Q J Nucl Med 2003;47:149-161.

  18. Ogata N, Nishikawa M, Nishimura T, Mitsuma Y, Matsumura M. Unbalanced vitreous levels of pigment epitheliumderived factor and vascular endothelial growth factor in diabetic retinopathy. Am J Opht 2002;134:348-353.

  19. Ogata N, Nishikawa M, Nishimura T, Mitsuma Y, Matsumura M. Inverse levels of pigment epithelium-derived factor and vascular endothelial growth factor in the vitreous of eyes with rhegmatogenous retinal detachment and proliferative vitreoretinopathy. Am J Opht 2002;133:851-852.

  20. Notari L, Miller A, Martínez A, Amaral J, Ju M, Robinson G, et al. Pigment Epithelium-Derived Factor is a Substrate for Matrix Metalloproteinase Type 2 and Type 9: Implications for Down regulation in Hypoxia. Invest Ophthalmol Vis Sci 2005;46:2736-2747.

  21. Stellmach V, Crawford SE, Zhou W, Bouck N. Prevention of ischemia-induced retinopathy by the natural ocular antiangiogenic agent pigment epithelium-derived factor. Proc Natl Acad Sci USA 2001;98:2593-2597.

  22. Matsuoka M, Ogata N, Otsuji T, Nishimura T, Takahashi K, Matsumura M. Expression of pigment epithelium derived factor and vascular endothelial growth factor in choroidal neovascular membranes and polypoidal choroidal vasculopathy. Br J Ophthalmol 2004;88:809-815.

  23. Noma H, Funatsu H, Yamashita H, Kitano S, Mishima HK, Hori S. Regulation of Angiogenesis in Diabetic Retinopathy: Possible Balance Between Vascular Endothelial Growth Factor And Endostatin. Arch Ophthalmol 2002;120:1075- 1080.

  24. Gao G, Li Y, Gee S, Dudley A, Fant J, Crosson C, et al. Down-regulation of Vascular Endothelial Growth Factor and Up-Regulation of Pigment Epithelium-derived Factor: A Possible Mechanism for the Anti-Angiogenic Activity of Plasminogen Kringle 5. J Biolog Chemistry 2002;277:9492- 9497.

  25. Cosano Povedano A. Old Methods, New Applications. Arch Bronconeumologia 2010;46:109-110.

  26. Li Y, Wang F, Wang H. Cell death along single microfluidic channel after freeze-thaw treatments. Biomicrofluidics 2010;4:014111. doi: 10.1063/1.3324869.

  27. Gage AA. History of cryosurgery. Semin Surg Oncol 1998;14:99-109.

  28. Whittaker DK. Mechanisms of tissue destruction following cryosurgery. Ann R Coll Surg Engl 1984;66:313-318.

  29. Sabel MS. Cryo-immunology: A review of the literature and proposed mechanisms for stimulatory versus suppressive immune responses. Cryobiology 2009;58:1-11.

  30. Forest V, Peoc’h M, Campos L, Guyotat D, Vergnon JM. Benefit of a combined treatment of cryotherapy and chemotherapy on tumour growth and late cryo-induced angiogenesis in a non-small-cell lung cancer model. Lung Cancer 2006;54:79-86.

  31. Pusztaszeri M, Vlastos G, Kinkel K, Pelte MF. Histopathological study of breast cancer and normal breast tissue after magnetic resonance-guided cryotherapy ablation. Cryobiology 2007;55:44-51.

  32. Pearce IA, Pennie FC, Gannon LM, Weindling AM, Clark DI. Three year visual outcome for treated stage 3 retinopathy of prematurity: cryotherapy versus laser. Br J Ophthalmol 1998;82:1254-1259.

  33. Gage AA, Baust JM, Baust JG. Experimental cryosurgery investigations in vivo. Cryobiology 2009;59:229-243.

  34. Maccini M, Sehrt D, Pompeo A, Chicoli FA, Molina WR, Kim FJ. Biophysiologic considerations in cryoablation: a practical mechanistic molecular review. Int Braz J Urol 2011;37:693- 696.

  35. Robilotto AT, Baust JM, Van Buskirk RG, Gage AA, Baust JG. Temperature-dependent activation of differential apoptotic pathways during cryoablation in a human prostate cancer model. Prostate Cancer Prostatic Dis 2013;16:41-49.

  36. Kimura M, Rabbani Z, Mouraviev V, Tsivian M, Vujaskovic Z, Satoh T, et al. Morphology of hypoxia following cryoablation in a prostate cancer murine model: Its relationship to necrosis, apoptosis and, microvessel density. Cryobiology 2010;61:148-154.

  37. Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nat Med 2003;9:669-676.

  38. Shao H, Schvartz I, Shaltiel S. Secretion of pigment epithelium- derived factor. Mutagenic study. Europ J Biochem/ FEBS 2003;270:822-831.

  39. Zhang SX, Wang JJ, Gao G, Parke K, Ma JX. Pigment epithelium- derived factor down regulates vascular endothelial growth factor (VEGF) expression and inhibits VEGF-VEGF receptor 2 binding in diabetic retinopathy. J Mol Endocrinol 2006;37:1-12.

  40. Ho TC, Chen SL, Shih SC, Chang SJ, Yang SL, Hsieh JW, et al. Pigment Epithelium-derived Factor (PEDF) Promotes Tumor Cell Death by Inducing Macrophage Membrane Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL). J Biolog Chem 2011;286:35943-35954.




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