Neri VP, Torres FV, Vielma VAV, Gaona AR

Language: Spanish
References: 24
Page: 97-103
PDF size: 740.99 Kb.

ABSTRACT

Background: Sperm capacitation is a calcium dependentprocess and is related to a cascade of biochemical changes. Capacitated sperm may respond to receptors of zona pellucida and increase even more the intracellular calcium level and with high levels of progesterone in the female genital tract acrosomal reaction is induced.
Objective: To determine the effect of incubation temperature on the spontaneous acrosomal reaction in capacitated seminal samples before assisted reproduction procedures.
Material and method: A prospective study was done with semen samples obtained from 10 normosperm patients with three to six days of sexual abstinence; fura ff-AM fluorescent optical marker was used, which is able to detect the increased intracellular calcium. Each sample was divided into two equal fractions: capacitated samples incubated at environment temperature (25ºC) and capacitated samples incubated at 37ºC.
Results: There was no significant difference in the spontaneous acrosomal reaction rate between capacitated seminal samples maintained at environment temperature and those incubated at 37ºC, even after four hours of capacitation; however, spontaneous acrosomal reaction tended slightly to be higher in samples incubated at 37ºC, because after progesterone addition, calcium influx was lower.
Conclusions: Even though temperature is important for sperm capacitation, it is not for acrosomal reaction; thus sperm maintained at 25ºC have the same probability to do an acrosomal reaction that those incubated at 37ºC.

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