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2013, Number 4

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Rev Cubana Plant Med 2013; 18 (4)

In vitro determination of the antioxidant capacity of extracts and phenolic compounds from Ugni molinae Turcz. leaves

Avello LM, Pastene NE, González RM, Bittner BM, Becerra AJ

Language: English
References: 40
Page: 596-608
PDF size: 293.36 Kb.


ABSTRACT

Introduction: Ugni molinae Turcz., (Myrtaceae) is a plant that is distributed in central-southern of Chile, including Juan Fernández Islands. The leaves have been used in folk medicine for diarrhea and oral infections due to their astringent and antiseptic properties.
Objective: antioxidant activity of two extracts (ethyl acetate and methanol) from U. molinae leaves was assessed in several assays.
Methods: HPLC (high performance liquid chromatography) for chemical analysis and in vitro antioxidant methods.
Results: HPLC profiling of both extracts showed the presence of flavan-3-ols (catechin) and gallic acid, flavonoids and caffeic acid derivates as main constituents. These extracts showed significant activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH), cupric ion reducing antioxidant capacity (CUPRAC) and hydroxyl radical assays. It is noteworthy that bleaching of b-carotene-linoleate liposomes and copper-induced oxidation of human LDL were prevented by both extracts.
Conclusions: results suggest that polyphenol-rich extracts of U. molinae could slow down lipid peroxidation and limit free radical damage.


REFERENCES

  1. Ivanova D, Gerova D, Chervenkov T, Yankova T. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. J Ethnopharmacol. 2005;96:145-50.

  2. Hoffmann A. Flora silvestre de Chile, zona Araucana. Chile: Fundación Claudio Gay; 1991. p. 160.

  3. Aguirre MC, Delporte C, Backhouse N, Erazo S, Letelier ME, Cassels BK, et al. Topical anti-inflammatory activity of 2 alpha-hydroxy pentacyclic triterpene acids from the leaves of Ugni molinae. Bioorganic Medicinal Chemistry. 2006;14:5673-7.

  4. Rubilar M, Pinelo M, Ihl M, Scheuermann E, Sineiro J, Nuñez MJ. Murta leaves (Ugni molinae Turcz.) as a source of antioxidant polyphenols. J Agricultural Food Chemistry. 2006;54:59-64.

  5. Devany S, Adams-Huet B, Jialal I. Comparison of rr-alpha-tocopherol and all-racemic alpha-tocopherol on LDL oxidation. Arteriosclerosis, Thrombosis, Vascular Biology. 1997;17:2273-9.

  6. Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organism. Physiological Reviews. 1979;59:527-605.

  7. McCall MR, Frei B. Can antioxidant vitamins materially reduce oxidative damage in humans? Free Radical Research Communications. 1999;26:1034-53.

  8. Sohal RS, Weindruch R. Oxidative stress, caloric restriction and aging. Science. 1996;273:59-63.

  9. Steinbrecher UP. Beyond cholesterol: modification of low density lipoprotein that increases its atherogenicity. J Biological Chemistry. 1987;262:3603-10.

  10. Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature. 1993;362:801-9.

  11. Palinski W, Rosenfeld ME, Yla-Hertuala S, Gurther GC, Socher SA, Butler SW, et al. Proceedings of the National Academy of Sciences of the United States of America. 1989;86:1372-6.

  12. Parthasarathy S, Satinberg D, Witztum JL. The role of oxidized low density lipoproteins in the pathogenesis of atherosclerosis. Annual Review Medicine. 1992;43:219-25.

  13. Avello M, Pastene E. Actividad antioxidante de infusos de Ugni molinae Turcz. (Murtilla). Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 2005;4:33-9.

  14. Arora A, Nair MG, Strasbur M. Structure-activity relationships for antioxidant activities of a series of flavonoids in a liposomal system. Free Radical Research Communications. 1998;24:1355-60.

  15. Ellnain-Wojtaszek M, Kruczynski Z, Kasprzak J. Investigation of the free radical scavenging activity of Ginkgo biloba L. leaves. Fitoterapia. 2003;74:1-6.

  16. Domínguez X. Métodos de investigación fitoquímica. México: Limusa; 1973. p. 81-149.

  17. Shinoda J. A new biologically active flavone glycoside from the roots of Cassia fistula Linn. J Pharmaceutical Society Japan. 1928;48:214.

  18. Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agricultural Food Chemistry. 1998;46:4113-7.

  19. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American J Enology Viticultur. 1965;16:144-58.

  20. Motamed B, Bohm JL, Hennequin D, Texier H, Mosrati R, Barillier D. Development of an HPLC method for the determination of phenolic by-products: optimization of the separation by means of the experimental designs methodology. European J Analytical Chemistry. 2000;28:592-9.

  21. Navarro MP, Montilla A, Marín J, Jiménez MP. Free radical scavenger and antihepatotoxic activity of Rosmarinus tomentosus. Planta Medica. 1993;59:312-3.

  22. Apak R, Güclü K, Demirata BM, Özyürek SE, Celik B, Bektasoglu K, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules. 2007;12:1496-547.

  23. Halliwell B, Gutteridge J, Aruoma OI. The deoxyribose method: a simple «test-tube» assay for determination of rate constants for reactions of hydroxyl radicals. Analytical Biochemistry. 1987;165:215-9.

  24. Winterbourn CC, Sutton H. Iron and xanthine oxidase catalyze formation of an oxidant species distinguishable from OH: comparison with the Haber-Weiss reaction. Biochemical Biophysical Research Communications. 1986;244:227-341.

  25. Schumaker VN, Puppione D. Sequential flotation ultracentrifugation. Methods Enzymology. 1986;128:155-70.

  26. Gugliucci A. Antioxidant effects of Ilex paraguariensis: induction of decreased oxidability of human LDL in vivo. Biochemical Biophysical Research Communications. 1996;224:338-44.

  27. Esterbauer H, Striegl G, Puhl H, Rotheneder M. Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Radical Biology Medicine. 1989;6:67-75.

  28. Steinberg D, Parthasarathy S, Carew TE, Khoo JC, Witztum JL. Beyond cholesterol: modification of low density lipoproteins that increase its atherogenicity. New England J Medicine. 1989;320:915-24.

  29. Noble RP. Electrophoretic separation of plasma lipoproteins in agarose gel. J Lipid Research. 1968;9:693-700.

  30. Delporte C, Backhouse N, Inostroza V, Aguirre MC, Peredo N, Silva X, et al. Analgesic activity of Ugni molinae (murtilla) in mice models of acute pain. J Ethnopharmacol. 2007;112:162-5.

  31. Belinky PA, Aviram M, Fuhrman B, Rosenblat M, Vaya J. The antioxidative effects of the isoflavan glabridin on endogenous constituents of LDL during its oxidation. Atherosclerosis. 1998;137:49-61.

  32. Khan MA, Shahidi F. Oxidative stability of stripped and nonstripped borage and evening primrose oils and their emulsions in water. J American Oil Chemists' Society. 2000;77:963-8.

  33. Frankel E. Food antioxidants and phytochemicals: Present and future perspectives. European J Lipid Science Technology. 1999;101:450-5.

  34. Porter WL. Paradoxical behavior of antioxidants in food and biological systems. Toxicology Industrial Health. 1993;9:93-122.

  35. Cox DA, Cohen ML. Effects of oxidized low-density lipoprotein on vascular contraction and relaxation: clinical and pharmacological implications in atherosclerosis. Pharmacological Reviews. 1996;48:3-19.

  36. Kellner BM, Cadenhead DA. Monolayer studies of hydroxyhexadecanoic acids. J Colloid Interface Science. 1977;63:452-60.

  37. Kellner BM, Cadenhead DA. Monolayer studies of methylhydroydecanoates. Chemistry Physics Lipids. 1978;23:41-8.

  38. Niki E. Assessment of antioxidant capacity in vitro and in vivo. Free Radical Biology Medicine. 2010;49:503-15.

  39. Uchida K, Toyokuni S, Nishikawa K, Kawakishi S, Oda H, Hiai H, et al. Michael addition-type 4-hydroxy-2-nonenal adducts in modified low-density lipoproteins: markers for atherosclerosis. Biochemistry. 1994;33:12487-94.

  40. Saura-Calixto F, Serrano J, Goñi I. Intake and bioaccessibility of total polyphenols in a whole diet. Food Chemistry. 2007;101:492-501.




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