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ISSN 1561-3054 (Electronic)

2013, Number 3

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Rev Cubana Med Trop 2013; 65 (3)

Efficacy of pyriproxyfen in reference strains of Aedes aegypti susceptible and resistant to temephos

Rodríguez CMM, Terán ZMC, Bisset LJA, Ricardo LY, French PL, Fuentes LI

Language: Spanish
References: 22
Page: 339-349
PDF size: 240.24 Kb.


ABSTRACT

Introduction: intensive use of chemical larvicide temephos (abate) in Cuba has resulted in the development of resistance mechanisms in Aedes (Stegomyia) aegypti (Linnaeus, 1762). Therefore, there is an urgent need to consider other control alternatives, such as pyriproxyfen, a growth inhibitor.
Objectives: determine the efficacy of pyriproxyfen as larvicide, pupicide and adulticide in reference strains of Aedes aegypti and establish its effect on reproduction.
Methods: the study was based on a strain of reference Aedes aegypti susceptible to temephos (Rockefeller) and a strain resistant to temephos (SANtemF13). Pyriproxyfen was evaluated with bioassays recommended by the World Health Organization. Determination was made of the effect of pyriproxyfen on reproduction by measuring fecundity and egg viability (fertility) in larvae of the Rockefeller strain surviving sublethal doses of pyriproxyfen.
Results: in the Rockefeller strain, pyriproxyfen was larvicidal in 32 %, pupicidal in 21 % and adulticidal in a mere 1 %. In the SANtem F13 strain, it was larvicidal in 31 %, pupicidal in 12 % and adulticidal in 1 %. Analysis of variance between ranges of lethal doses for larvae, pupas and adults of the reference strains Rockefeller and SANtem F13 did not show any significant differences (p= 0.000001). Pyriproxyfen was found to be an excellent inhibitor of adult emergence in the strains of Aedes aegypti studied when used at low concentrations, below those recommended by the World Health Organization for mosquitoes of the genus Aedes. It also showed a strong sublethal effect and affected the fertility of adults in laboratory conditions.
Conclusion: growth inhibitor pyriproxyfen is a good alternative to be included among integrated control strategies against Aedes aegypti in Cuba without affecting its efficacy, due to the resistance that this species has developed to larvicide temephos.


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