Rosety-Rodríguez M, Camacho A, Rosety MÁ, Fornieles G, Diaz AJ, Rosety I, García N, Pardo A, Ordonez FJ

Language: Spanish
References: 24
Page: 331-335
PDF size: 143.30 Kb.

ABSTRACT

Introduction. Recent studies concluded long-term training programs have improved antioxidant system in young and adults diabetic rats. However, to our knowledge, little attention has been paid to elderly individuals. Objective. To assess the influence of a shorter training program in reducing oxidative damage in elderly diabetic rats. Material and methods. Twenty-four male homozygous Zucker diabetic fatty rats (Gmi, fa/fa) aged 18-weeks with an average weight of 370- 450 g were purchased. After a 2-week period of environmental adaptation, animals were randomly distributed into exercised group (n = 12) that performed a 6-week swimming training protocol and sedentary group (n = 12). Animals were sacrificed 24-h after the last exercise session under anesthesia. Serum metabolic profile was determined. Lipid oxidation, expressed as malondialdehyde and protein oxidation, expressed as carbonyl groups, were assessed in plasma samples. This protocol was approved by an Institutional Ethics Committee. Results. Exercised rats improved significantly their metabolic profile in comparison to controls. Plasma malondialdehyde (1.58 ± 0.39 vs. 2.06 ± 0.41 nmol/mL; p = 0.016) and carbonyl group levels (1.37 ± 0.33 vs. 1.62 ± 0.58 UA; p = 0.011) were also significantly lowered in exercised rats when compared to sedentary counterparts. Conclusions. A 6-week swimming training program reduced lipid and protein oxidation in elderly fatty diabetic rats. Further studies on this topic are required.

REFERENCES

1. Broedbaek K, Weimann A, Stovgaard ES, Poulsen HE. Urinary 8-oxo-7,8-dihydro-2’-deoxyguanosine as a biomarker in type 2 diabetes. Free Radic Biol Med 2011; 51: 1473-9.

2. Zheng F, Lu W, Jia C, Li H, Wang Z, Jia W. Relationships between glucose excursion and the activation of oxidative stress in patients with newly diagnosed type 2 diabetes or impaired glucose regulation. Endocrine 2010; 37: 201-08.

3. Kaneto H, Katakami N, Matsuhisa M, Matsuoka TA. Role of reactive oxygen species in the progression of type 2 diabetes and atherosclerosis. Mediators Inflamm 2010. Epub 2010 Feb 16.

4. Lee H, Chang H, Park JY, Kim SY, Choi KM, Song W. Exercise training improves basal blood glucose metabolism with no changes of cytosolic inhibitor B kinase or c-Jun N-terminal kinase activation in skeletal muscle of Otsuka Long-Evans Tokushima fatty rats. Exp Physiol 2011; 96: 689-98.

5. Qi Z, He J, Zhang Y, Shao Y, Ding S. Exercise training attenuates oxidative stress and decreases p53 protein content in skeletal muscle of type 2 diabetic Goto-Kakizaki rats. Free Radic Biol Med 2011; 50: 794-800.

6. Mehta R, del-Moral ME, Aguilar-Salinas CA. Epidemiology of diabetes in the elderly. Rev Invest Clin 2010; 62: 305-11.

7. Tessier D, Avila-Funes JA, Aguilar-Navarro S. Specific needs in the care of elderly with diabetes mellitus. Rev Invest Clin 2010; 62: 323-6.

8. Nilsen V, Bakke PS, Gallefoss F. Effects of lifestyle intervention in persons at risk for type 2 diabetes mellitus-results from a randomised, controlled trial. BMC Public Health 2011; 11: 893.

9. Király MA, Bates HE, Kaniuk NA, Yue JT, Brumell JH, Matthews SG, et al. Swim training prevents hyperglycemia in ZDF rats: mechanisms involved in the partial maintenance of beta-cell function. Am J Physiol Endocrinol Metab 2008; 294: 271-83.

10. Rosety-Rodriguez M, Ordonez FJ, Rosety I, Frias L, Rosety MA, Rosety JM, et al. 8-weeks training program attenuates mitochondrial oxidative stress in the liver of emotionally stressed rats. Histol Histopathol 2006; 21: 1167-70.

11. Karatas F, Karatepe M, Baysar A. Determination of free malondialdehyde in human serum by high-performance liquid chromatography. Anal Biochem 2002; 311: 76-9.

12. Lenaers E, De Feyter HM, Hoeks J, Schrauwen P, Schaart G, Nabben M, et al. Adaptations in mitochondrial function parallel, but fail to rescue, the transition to severe hyperglycemia and hyperinsulinemia: a study in Zucker diabetic fatty rats. Obesity (Silver Spring) 2010; 18: 1100-07.

13. Alemzadeh R, Tushaus K. Diazoxide attenuates insulin secretion and hepatic lipogenesis in zucker diabetic fatty rats. Med Sci Monit 2005; 11: BR439-48.

14. Ferreira L, Teixeira-de-Lemos E, Pinto F, Parada B, Mega C, Vala H, et al. Effects of sitagliptin treatment on dysmetabolism, inflammation, and oxidative stress in an animal model of type 2 diabetes (ZDF rat). Mediators Inflamm 2010: 592760 [Epub 2010 Jun 21].

15. Pold R, Jensen LS, Jessen N, Buhl ES, Schmitz O, Flyvbjerg A, et al. Long-term AICAR administration and exercise prevents diabetes in ZDF rats. Diabetes 2005; 54: 928-34.

16. de Lemos ET, Reis F, Baptista S, Pinto R, Sepodes B, Vala H, et al. Exercise training is associated with improved levels of Creactive protein and adiponectin in ZDF (type 2) diabetic rats. Med Sci Monit 2007; 13: 168-74.

17. Christ CY, Hunt D, Hancock J, Garcia-Macedo R, Mandarino LJ, Ivy JL. Exercise training improves muscle insulin resistance but not insulin receptor signaling in obese Zucker rats. J Appl Physiol 2002; 92: 736-44.

18. Ordonez FJ, Rosety I, Diaz-Ordonez AJ, Fornieles G, Camacho- Molina A, Rosety MA, et al. A 6-week training program increased muscle antioxidant system in elderly diabetic fatty rats. Med Sci Monit 2012; 18: BR346-50.

19. Perse M, Injac R, Strukelj B, Cerar A. Effects of high-fat mixed-lipid diet and exercise on the antioxidant system in skeletal and cardiac muscles of rats with colon carcinoma. Pharmacol Rep 2009; 61: 909-16.

20. Huang CC, Tsai SC, Lin WT. Potential ergogenic effects of Larginine against oxidative and inflammatory stress induced by acute exercise in aging rats. Exp Gerontol 2008; 43: 571-7.

21. Henriksen EJ, Diamond-Stanic MK, Marchionne EM. Oxidative stress and the etiology of insulin resistance and type 2 diabetes. Free Radic Biol Med 2011; 51: 993-9.

22. Király MA, Campbell J, Park E, Bates HE, Yue JT, Rao V, et al. Exercise maintains euglycemia in association with decreased activation of c-Jun NH2-terminal kinase and serine phosphorylation of IRS-1 in the liver of ZDF rats. Am J Physiol Endocrinol Metab 2010; 298: 671-82.

23. Nilsen V, Bakke PS, Gallefoss F. Effects of lifestyle intervention in persons at risk for type 2 diabetes mellitus-results from a randomised, controlled trial. BMC Public Health 2011; 11: 893.

24. Ferrer-García JC, Sánchez López P, Pablos-Abella C, Albalat- Galera R, Elvira-Macagno L, Sánchez-Juan C, et al. Benefits of a home-based physical exercise program in elderly subjects with type 2 diabetes mellitus. Endocrinol Nutr 2011; 58: 387-94.