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2013, Number 2

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Rev Hematol Mex 2013; 14 (2)

Usefulness of PFA-100 in a Colombian Population, as a Screening Method for Von Willebrand Disease and Function Platelet Disorders

Lombana MA, Ramos-Ramos G, Milena TA

Language: Spanish
References: 44
Page: 71-77
PDF size: 362.85 Kb.


ABSTRACT

Background: The evaluation of inherited disorders of platelet function in clinical practice is hampered by the use of screening methods with poor reproducibility and operational performance. Recently the use of evidence as the PFA-100 seems to be a more appropriate method. In Colombia there is not quantified or published experience in this regard.
Objective: The main objective of this study is to present the first Colombian experience of the diagnostic performance of the PFA- 100 in patients with suspected Von Willebrand disease (VWD) and non-pharmacological functional platelet disorders.
Materials and methods: We performed a cross-sectional study of validation of diagnostic tests, with prospective data collection. We included patients with suspected VWD, functional platelet disorders and a group of healthy controls.
All patients underwent PFA-100 test independently, with simultaneous determination of Von Willebrand antigen, ristocetin cofactor, factor VIII and platelet aggregation. We determined the sensitivity, specificity, LR + and LR-of PFA-100 and bleeding time as an alternative method.
Results: 38 subjects were included from Bogota. The PFA-100 had a sensitivity of 90%, negative LR 0,12, a specificity of 85.7% and positive LR 6,2. The bleeding time had a sensitivity of 40% and negative LR 0,6.
Conclusions: In patients with suspected VWD or platelet function disorders using PFA-100 in our study group had a high diagnostic yield. It is recommended the use of PFA-100 as a screening method in patients with clinical suspicion of VWD or platelet function defects, not with bleeding time due to its poor sensitivity.


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