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ISSN 1561-3062 (Electronic)

2013, Number 3

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Revista Cubana de Obstetricia y Ginecología 2013; 39 (3)

Implications of HIV transmission from human sperm during fertilization

Sánchez JA, Cardona-Maya W, Rugeles MT

Language: Spanish
References: 30
Page:
PDF size: 180.31 Kb.


ABSTRACT

Introduction: seminal leukocytes are considered the source of HIV infection during sexual transmission, although the interaction between sperm and virus has been described.
Objective: the aim of this study was to determine the ability of sperm from HIVpositive individuals to transfer the virus to macrophages.
Methods: pure sperm from 14 HIV-1 infected patients treated or not with trypsin were cocultured with macrophages. Cell interaction was evaluated by light microscopy. Nested-PCR for the env gene from sperm and cocultures DNA was performed.
Results: macrophages sperm interaction through head, neck and tail was found. Proviral DNA was not detected within sperm DNA. However, the env gene was detected in DNA from the cocultures at 4 and 24 hours in 50% and 87.5% of those treated with trypsin and in 43% and 28.6% of the non treated cultures, respectively.
Conclusion: the virus enters the sperm, and this cell is capable of transferring the virus to macrophages. Thus, during natural or assisted fertilization, viral particles might enter into the oocyte, establishing a productive infection in the embryo, and transmitting the infection to the mother. Trypsin could enhance viral transmission by facilitating entry of virions into macrophages.


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